A novel thermostable, alkaline pectate lyase from Bacillus tequilensis SV11 with potential in textile industry

Chiliveri, Swarupa Rani; Linga, Venkateswar Rao

doi:10.1016/j.carbpol.2014.04.065

Cited by 40 publications

(16 citation statements)

References 28 publications

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“…At 60°C, it maintained 47 % of residual activity for 45 min. The characteristics of alkaline pectinase of B. mojavensis I4 are comparable to those of B. halodurans M29 that was optimally active at pH 10.0 and 80°C (Mei et al 2013), the pectinase from B. pumilus dcsr1 reported to exhibits maximal activity at pH 10.5 and 50°C (Sharma and Satyanarayana 2006) and also pectate lyase from B. tequilensis SV11 which kept maximum activity at pH 9.0 and 60°C (Chiliveri and Linga 2014).…”

Section: Biochemical Propertiesmentioning

confidence: 69%

Assessment of pectinase production by Bacillus mojavensis I4 using an economical substrate and its potential application in oil sesame extraction

et al. 2015

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Carrot (Daucus carota) peels, local agricultural waste product, is rich in lignocellulolytic material, including pectin which can act as an inducer of pectinase production. Pectinolytic enzymes production by Bacillus mojavensis I4 was studied in liquid state fermentation using carrot peel as a substrate. Medium composition and culture conditions for the pectinase production by I4 were optimized using two statistical methods: Taguchi design was applied to find the key ingredients and conditions for the best yield of enzyme production and The Box-Behnken design was used to optimize the value of the four significant variables: carrot peels powder, NH4Cl, inoculum size and incubation time. The optimal conditions for higher production of pectinase were carrot peels powder 6.5 %, NH4Cl 0.3 %, inoculum level 3 % and cultivation time 32 h. Under these conditions, the pectinase experimental yield (64.8 U/ml) closely matched the yield predicted by the statistical model (63.55 U/ml) with R (2) = 0.963. The best pectinase activity was observed at the temperature of 60 °C and at pH 8.0. The enzyme retained more than 90 % of its activity after 24 h at pH ranging from 6.0 to 10.0. The enzyme preserved more than 85 % of its initial activity after 60 min of pre-incubation at 30-40 °C and more than 67 % at 50 °C. The extracellular juice of I4 was applied in the process of sesame seeds oil extraction. An improvement of 3 % on the oil yield was obtained. The findings demonstrated that the B. mojavensis I4 has a promising potential for future use in a wide range of industrial and biotechnological applications.

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Section: Biochemical Propertiesmentioning

confidence: 69%

Assessment of pectinase production by Bacillus mojavensis I4 using an economical substrate and its potential application in oil sesame extraction

et al. 2015

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“…strain KSM-P15; and Pel SWU from Bacillus spp. RN1 [ 13 , 14 , 15 , 16 , 17 , 18 ]. Here, P. polymyxa exhibited pectin-degradation activity.…”

Section: Resultsmentioning

confidence: 99%

Screening of a Novel Polysaccharide Lyase Family 10 Pectate Lyase from Paenibacillus polymyxa KF-1: Cloning, Expression and Characterization

et al. 2018

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Pectate lyase (EC 4.2.2.2) catalyzes the cleavage of α-1,4-glycosidic bonds of pectin polymers, and it has potential uses in the textile industry. In this study, a novel pectate lyase belonging to polysaccharide lyase family 10 was screened from the secreted enzyme extract of Paenibacillus polymyxa KF-1 and identified by liquid chromatography-MS/MS. The gene was cloned from P. polymyxa KF-1 genomic DNA and expressed in Escherichia coli. The recombinant enzyme PpPel10a had a predicted Mr of 45.2 kDa and pI of 9.41. Using polygalacturonic acid (PGA) as substrate, the optimal conditions for PpPel10a reaction were determined to be 50 °C and pH 9.0, respectively. The Km, vmax and kcat values of PpPel10a with PGA as substrate were 0.12 g/L, 289 μmol/min/mg, and 202.3 s−1, respectively. Recombinant PpPel10a degraded citrus pectin, producing unsaturated mono- and oligogalacturonic acids. PpPel10a reduced the viscosity of PGA, and weight loss of ramie (Boehmeria nivea) fibers was observed after treatment with the enzyme alone (22.5%) or the enzyme in combination with alkali (26.3%). This enzyme has potential for use in plant fiber processing.

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“…In comparison to harsh alkaline conditions employed traditionally, treatment of textile fibers by xylan-and pectindegrading enzymes might not affect the cellulosic part of the fabric and avoid fiber damage and wastewater pollution (Rouette 2001). Similarly, Chiliveri and Linga (2014) have reported that scouring using specific enzymes is the best option compared to the traditional scouring method. Singh et al (2016) also observed that bioscouring is more efficient than alkaline scouring.…”

Section: Optimization Of Bioscouring Parametersmentioning

confidence: 99%

“…The use of microbial enzymes for textile fiber processing has increased significantly, as they cause less damage to fibers, improve fabric quality, require less energy and use less environment-damaging chemicals. (Chiliveri and Linga 2014;Dhiman et al 2008;Joshi et al 2013;Mojsov 2016;Shanthi and Krishnabai 2013). Bioscouring produces good quality fibers with more softness and better strength.…”

Section: Introductionmentioning

confidence: 99%

A sustainable and green process for scouring of cotton fabrics using xylano-pectinolytic synergism: switching from noxious chemicals to eco-friendly catalysts

et al. 2018

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The objective of this research was to develop an appropriate, eco-friendly, cost-effective bioscouring methodology for removing natural impurities from cotton fabric. Maximum bioscouring was achieved using 5.0 IU xylanase and 4.0 IU pectinase with material to liquid ratio of 1:15 in a 50 mM buffer (glycine-NaOH buffer, 1.0 mM EDTA and 1% Tween-80, pH 8.5) with a treatment time of 60 min at 50 °C and an agitation speed of 60 rpm. The bioscoured cotton fabrics showed a gain of 1.17% in whiteness, 3.23% in brightness and a reduction of 4.18% in yellowness in comparison to fabric scoured with an alkaline scouring method. Further, after bleaching, the whiteness, brightness and tensile strength of the bioscoured fabrics were increased by 2.18, 2.33 and 11.74% along with a decrease of 4.61% in yellowness of bioscoured plus bleached fabrics in comparison to chemically scoured plus bleached fabrics. From the results, it is clear that bioscouring is more efficient, energy saving and an eco-friendly process and has the potential to replace the environment-damaging scouring process with the xylano-pectinolytic bioscouring process.

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A novel thermostable, alkaline pectate lyase from Bacillus tequilensis SV11 with potential in textile industry

Cited by 40 publications

References 28 publications

Assessment of pectinase production by Bacillus mojavensis I4 using an economical substrate and its potential application in oil sesame extraction

Assessment of pectinase production by Bacillus mojavensis I4 using an economical substrate and its potential application in oil sesame extraction

Screening of a Novel Polysaccharide Lyase Family 10 Pectate Lyase from Paenibacillus polymyxa KF-1: Cloning, Expression and Characterization

A sustainable and green process for scouring of cotton fabrics using xylano-pectinolytic synergism: switching from noxious chemicals to eco-friendly catalysts

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