A novel treatment strategy targeting Aurora kinases in acute myelogenous leukemia

Abstract

The Aurora kinases play an important role in chromosome alignment, segregation, and cytokinesis during mitosis.

Retracted: Overexpression of Aurora A by loss of CHFR gene expression increases the growth and survival of HTLV‐1‐infected T cells through enhanced NF‐κB activity

“…These results are in agreement with the recent study of Ikezoe et al 43 who showed aberrant expression of Aurora Cells were harvested 48 hr after transfection. Luciferase activity was analyzed as described in ''Material and methods'' section.…”

Copper reverses cardiomyocyte hypertrophy through vascular endothelial growth factor-mediated reduction in the cell size

“…Determination of mitotic activities of cardiomyocytes under different treatments was done as described previously [13], with some modifications. Briefly, the cells were trypsinized, washed with PBS, and fixed in 75% ethanol at 4 °C for at least 18 h. Immunofluorescence staining with an Alexa Fluor 488 conjugated antibody to phosphorylated Histone-3 (Ser10, 1:10, Cell Signaling Technology, Inc., Danvers, MA), which is a mitosis-specific marker, was performed on the fixed cardiomyocytes.…”

Analysis of Aurora B kinase in non-Hodgkin lymphoma

“…14 We have shown recently that hematological malignant cells including those from acute myeloid leukemia (AML), acute lymphoblastic leukemia (ALL), as well as chronic myeloid leukemia aberrantly expressed Aurora A and B kinases. 15 In addition, we have shown that ZM447439, a novel and selective inhibitor of Aurora A and B kinases, effectively induced growth arrest and apoptosis of a variety of types of leukemia cells. 15 Studies conducted by other groups noted that the phenotype of HeLa cells after their exposure to ZM447439 was similar to that induced by knock down of the Aurora B kinase gene.…”

“…15 In addition, we have shown that ZM447439, a novel and selective inhibitor of Aurora A and B kinases, effectively induced growth arrest and apoptosis of a variety of types of leukemia cells. 15 Studies conducted by other groups noted that the phenotype of HeLa cells after their exposure to ZM447439 was similar to that induced by knock down of the Aurora B kinase gene. 16 We, therefore, hypothesized that Aurora B might be a more favorable molecular target for cancer therapy than Aurora A.…”