A Novel Turn‐on Fluorescent Probe for Highly Selective Detection of Phosphate Ion in Living Cell

Abstract

A novel fluorescent probe was synthesized and studied. Fluorescent probe 1 undergoes a unique Pi induced hydrolytic reaction in DMSO‐ HEPES at physiological pH that produces a colorimetric change associated with a 76 nm red‐shift in the UV‐vis absorption maximum and up to a 210‐fold enhancement in the fluorescence intensity. It has successfully been used to stain and record microscopy images of HeLa cells. Moreover, the mechanistic proposal that these spectroscopic changes are associated with the reaction of Pi and probe 1 to form 2‐(2‐hydroxyphenyl)benzothiazole gains support from the results of mass spectrometry studied.

A Novel Ratiometric Fluorescent Probe for Hypobromous Acid Detection in Living Cells

“…In comparison with other biological detection technologies, fluorescent probe has attracted general attention because of the high selectivity and sensitivity with minimal invasiveness [21–23] . Furthermore, benzothiazole is widely used as a fluorophore in fluorescent probes because of its large delocalized π bond and rigid planar structure [24] . Herein, we reported a new fluorescent probe HB‐2 based on benzothiazole.…”

“…[21][22][23] Furthermore, benzothiazole is widely used as a fluorophore in fluorescent probes because of its large delocalized π bond and rigid planar structure. [24] Herein, we reported a new fluorescent probe HB-2 based on benzothiazole. The probe HB-2 exploits increase of the intensity of fluorescence after the addition of HBrO.…”

Phosphate Assay Kit in One Cell for Electrochemical Detection of Intracellular Phosphate Ions at Single Cells

“…To measure phosphate ions in the biological samples, a series of chemosensors were synthesized that are capable of detecting inorganic phosphates at a physiological pH (Hatai et al, 2012; Liu et al, 2016). However, very few fluorescent probes have been synthesized to recognize phosphate ions inside the cells, which are partially due to the poor selectivity for phosphate in presence of structurally similar anions in cells (Guo et al, 2015; Zhang et al, 2018).…”

A Dual-Control Strategy by Phosphate Ions and Local Microviscosity for Tracking Adenosine Triphosphate Metabolism in Mitochondria and Cellular Activity Dynamically

“…Instrumentation. 1 H NMR and 13 C NMR spectra were taken on a 500 MHz NMR spectrometer (Bruker, Karlsruhe, Germany) using DMSO-d 6 as solvent, and chemical shifts were reported in ppm downfield relative to tetramethylsilane (TMS, δ scale with solvent resonances as internal standards). HR-MS were measured by Xevo G2-XS Q Tof mass spectrometer.…”