A novel vector for a rapid generation of fiber-mutant adenovirus based on one step ligation and quick screening of positive clones

Wang, Dongyang; Liu, Shihai; Mao, Qinwen; Zhao, Junli; Xia, Haibin

doi:10.1016/j.jbiotec.2011.02.001

Cited by 5 publications

(1 citation statement)

References 39 publications

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“…The sites of choice for targeting ligand incorporation are the fiber knob’s HI loop, which connects the ß-strands H and I, and the C-terminus of the protein [86,90,91,92,93,94,95,96]. These findings indicated that ligands whose sizes exceed 25 to 30 amino acid residues cannot be configured into the carboxy-terminus of the fiber, as they destabilize the fiber structure [85] and, thus, limit the range of potential ligand candidates to short peptides.…”

Section: Methods To Alter the Natural Ad Vector Tropismmentioning

confidence: 99%

Peptide-Based Technologies to Alter Adenoviral Vector Tropism: Ways and Means for Systemic Treatment of Cancer

Reetz

Herchenröder

Pützer

2014

Viruses

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Due to the fundamental progress in elucidating the molecular mechanisms of human diseases and the arrival of the post-genomic era, increasing numbers of therapeutic genes and cellular targets are available for gene therapy. Meanwhile, the most important challenge is to develop gene delivery vectors with high efficiency through target cell selectivity, in particular under in situ conditions. The most widely used vector system to transduce cells is based on adenovirus (Ad). Recent endeavors in the development of selective Ad vectors that target cells or tissues of interest and spare the alteration of all others have focused on the modification of the virus broad natural tropism. A popular way of Ad targeting is achieved by directing the vector towards distinct cellular receptors. Redirecting can be accomplished by linking custom-made peptides with specific affinity to cellular surface proteins via genetic integration, chemical coupling or bridging with dual-specific adapter molecules. Ideally, targeted vectors are incapable of entering cells via their native receptors. Such altered vectors offer new opportunities to delineate functional genomics in a natural environment and may enable efficient systemic therapeutic approaches. This review provides a summary of current state-of-the-art techniques to specifically target adenovirus-based gene delivery vectors.

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Section: Methods To Alter the Natural Ad Vector Tropismmentioning

confidence: 99%

Peptide-Based Technologies to Alter Adenoviral Vector Tropism: Ways and Means for Systemic Treatment of Cancer

Reetz

Herchenröder

Pützer

2014

Viruses

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Recent Progress in Strategies for Adenovirus Mediated Therapeutic Cell Targeting

Herchenröder

Reetz

Pützer

2016

Regenerative Medicine - From Protocol to Patient

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A one-step ligation system for rapid generation of armed, conditionally-replicating adenoviruses

Xia

Zhao

et al. 2013

Biotechnol Lett

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Conditionally-replicating adenoviruses (CRAd) are becoming a promising strategy for cancer therapy. However, the generation of CRAd is complicated and time-consuming, especially for armed CRAd Ad5-D24. A one-step ligation system is now reported for rapid construction of an armed CRAd Ad5-D24 carrying two foreign transgenes. The system has the following features: (1) the CRAd backbone pCRAd5-D24-BS-Lacza contains two unique restriction sites between the fiber and E4 for easy introduction of a transgene expression cassette; and (2) a bi-directional promoter in pshuttle-BS-Dual allows for the expression of two independent transgenes. As proof of principle, Ad5-D24 CRAd carrying eGFP and mCherry was generated and examined. This novel ligation system will be a versatile and efficient tool in cancer viral gene therapy.

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A novel vector for a rapid generation of fiber-mutant adenovirus based on one step ligation and quick screening of positive clones

Cited by 5 publications

References 39 publications

Peptide-Based Technologies to Alter Adenoviral Vector Tropism: Ways and Means for Systemic Treatment of Cancer

Peptide-Based Technologies to Alter Adenoviral Vector Tropism: Ways and Means for Systemic Treatment of Cancer

Recent Progress in Strategies for Adenovirus Mediated Therapeutic Cell Targeting

A one-step ligation system for rapid generation of armed, conditionally-replicating adenoviruses

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