A novel WSSV responsive plasma protein from Litopenaeus vannamei contributes to hemocytes anti-apoptosis

Tao, Mengyuan; Liang, Yu-Qin; Zhang, Yueling; Wang, Fan

doi:10.1016/j.molimm.2020.02.007

Cited by 4 publications

(3 citation statements)

References 22 publications

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“…The supernatant samples were separated on SDS-PAGE and transferred to a polyvinylidene fluoride (PVDF) membrane as described before (10). In brief, the membranes were sequentially incubated with rabbit anti-CREG antibody, which is commercially prepared by GenScript, HRP-linked goat antirabbit antibody (Sangon Biotech, China), and Western Chemiluminescent HRP substrate (Millipore, Burlington, MA, USA).…”

Section: Sds-page and Western Blot Analysesmentioning

confidence: 99%

“…The RNA interference (RNAi) was performed as previously described with some modifications (10). Briefly, dsCREG and dsEGFP were in vitro transcribed using the ScriptMAX ® Thermo T7 transcription Kit (Toyobo, Japan) with specific primers (Table 1).…”

Section: Creg Knockdown Experimentsmentioning

confidence: 99%

“…Here, we have applied this species as an invertebrate model to explore conserved potential novel cytokines in metazoans. To achieve this, we have previously purified Penaeus vannamei low abundant proteins, identified them with mass spectrometry (9), and screened potential functional cytokines with bioinformatics and biochemical methods (10). In this study, a conserved CREGlike protein was identified in shrimp plasma.…”

Section: Introductionmentioning

confidence: 99%

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Shrimp Plasma CREG Is a Hemocyte Activation Factor

2021

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Cytokines are a class of immunoregulatory proteins that are secreted by cells. Although vertebrate cytokine, especially mammalian cytokine has been well studied for the past decades. Much less attention has been paid to invertebrate so that only some cytokines have been identified in invertebrates. We have chosen Peaneus vannamei as a model to explore novel invertebrate cytokines. To achieve this, we previously purified shrimp plasma low abundance proteins and identified more than 400 proteins with proteomics analyses. In this study, a cellular repressor of E1A-stimulated gene (CREG)-like protein, which is highly conserved from Drosophila melanogaster to Homo sapiens, was further characterized in shrimp plasma. We found that shrimp plasma CREG was a glycoprotein which was strongly induced in hemolymph at 8 h post-LPS injection. Further function experiment unveiled that recombinant shrimp CREG protein injection significantly increased phagocytic hemocyte and lysosome-high hemocyte proportion in hemolymph. After that, hemocytes from rEGFP- and rCREG-protein injected shrimps were subjected to transcriptome analyses, which revealed that shrimp CREG protein could comprehensively promote hemocyte maturation and activation. Taken together, our data clearly indicated that shrimp plasma CREG protein is a novel hemocyte activation factor, which is probably a conserved myeloid cell lineage activation factor from invertebrate to vertebrate.

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Section: Sds-page and Western Blot Analysesmentioning

confidence: 99%

Section: Creg Knockdown Experimentsmentioning

confidence: 99%