A numerical failure analysis of laser micromachining in various thermoplastics

Moghadasi, Kaveh; Tamrin, Khairul Fikri; Sheikh, Nadeem Ahmed; Jawaid, Mohammad

doi:10.1007/s00170-021-07428-1

Cited by 6 publications

(2 citation statements)

References 55 publications

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“…Laser micromachining and hot embossing are two promising technologies for fabricating microstructures on the Petri dish. Laser micromachining offers distinct advantages, including exceptional precision and precise spatial selectivity.…”

Section: Introductionmentioning

confidence: 99%

“…Currently, no study exists about the irreversibly bonded composite chip featuring a microstructured doublelayer system in the context of microfluidic cell culture. Laser micromachining 31 and hot embossing 32 are two promising technologies for fabricating microstructures on the Petri dish. Laser micromachining offers distinct advantages, including exceptional precision and precise spatial selectivity.…”

Section: ■ Introductionmentioning

confidence: 99%

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Composite Microfluidic Petri Dish-Chip (MPD-Chip) without Protein Coating for 2D Cell Culture

Yin,

Lu,

Liu

et al. 2023

Langmuir

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Hydrophilicity is a requisite attribute for the 2D cell culture substrate's surface, facilitating cell adhesion and spreading. Conventional poly(dimethylsiloxane) (PDMS) microfluidic chips necessitate protein coatings to enhance hydrophilicity; however, this approach is afflicted by issues of transient efficacy, interference with cell analysis, and high costs. This paper presents a protein-free microfluidic chip, termed a "microfluidic Petri dish-chip (MPD-chip)", integrating PDMS as the cover and a tissue culture-treated (TC-treated) Petri dish as the substrate. Microstructures are hot-embossed onto the Petri dish substrate using a silicon mold. This meticulous replication process serves to establish stable flow field dynamics within the chip. A simplified method for irreversible bonding, utilizing plasma activation and silylation, is proposed for affixing the PDMS cover onto the microstructured Petri dish substrate. The prepared composite chip exhibits remarkable tightness, boasting a notable bond strength of 2825 kPa. Furthermore, the composite microfluidic chip demonstrates the capability to withstand flow velocities of at least 200 μL/min, effectively meeting the required injection standards for both cell suspension and culture medium. SH-SY5Y and HeLa cells are cultured dynamically in the MPD-chip and control groups. Outcomes encompassing normalized cell density, cell adhesion area, and cell viability metrics unequivocally highlight the superiority of the MPD-chip in facilitating long-term two-dimensional (2D) cell cultures.

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Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%