A numerical investigation of drug extravasation using a tumour–vasculature microfluidic device

Li, Wei; Wang, Haofei; Kuruneru, Sahan Trushad Wickramasooriya; Wang, Tong; Li, Zhiyong; Zhao, Chun‐Xia; Gu, Yuantong

doi:10.1007/s10404-018-2165-y

Cited by 9 publications

(9 citation statements)

References 19 publications

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“…A finite-volume method (FVM) was used to solve the steady three-dimensional Navier–Stokes (N–S) governing flow equations. 52,53 The fluid was water with a density of 998.2 kg m −3 and a viscosity of 0.001003 kg m −1 s −1 . The pressure field and the velocity field were coupled with the SIMPLE algorithm.…”

Section: Methodsmentioning

confidence: 99%

Axons-on-a-chip for mimicking non-disruptive diffuse axonal injury underlying traumatic brain injury

Pan

et al. 2022

Lab Chip

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Section: Methodsmentioning

confidence: 99%

Axons-on-a-chip for mimicking non-disruptive diffuse axonal injury underlying traumatic brain injury

Pan

et al. 2022

Lab Chip

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“…The FLUENT code in ANSYS software 18.0 was adapted to obtain the flow structure in this microfluidic device and force distribution on axon surfaces. A finite-volume method (FVM) was used to solve the steady three-dimensional Navier–Stokes (N–S) governing flow equations 46,47 . The fluid was water with density of 998.2 kg/m 3 and the viscosity of 0.001003 kg/m·s.…”

Section: Methodsmentioning

confidence: 99%

Axons-on-a-Chip for Mimicking Non-Disruptive Diffuse Axonal Injury underlying Traumatic Brain Injury

Wang

et al. 2021

Preprint

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Diffuse axonal injury (DAI) is the most severe pathological feature of traumatic brain injury. However, how primary axonal injury is induced by mechanical stress and whether it could be mitigated remain unknown, largely due to the resolution limits of medical imaging approaches. Here we established an Axon-on-a-Chip (AoC) model for mimicking DAI and investigating its early cellular responses. By integrating computational fluid dynamics and microfluidic techniques, DAI was observed for the first time during mechanical stress, and a clear correlation between stress intensity and severity of DAI was elucidated. This AoC was further used to investigate the dynamic intracellular changes occurring simultaneously with stress, and identified delayed local Ca2+ surges escorted rapid disruption of periodic axonal cytoskeleton during the early stage of DAI. Compatible with high-resolution live-microscopy, this model hereby provides a versatile system to identify early mechanisms underlying DAI, offering a platform for screening effective treatments to alleviate brain injuries.

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“…24−26 Microfluidics allows precise manipulation of fluidics in microchannels to recreate the tumor microenvironment with better-mimicked key parameters such as blood flow and gradient of biochemical cues, thereby allowing cell culture and drug screening in more physiological relevant conditions. 27,28 The incorporation of hydrogel scaffolds enables coculture of multiple cell types in a three-dimensional (3D) matrix. 29 Moreover, the transparent microfluidic devices allow real-time assessment of cell−cell interaction and on-chip functional assays to characterize drug responses, which is particularly favorable for evaluating macrophage-based drug carriers.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Compared to conventional models, microfluidic-based models have attracted broad research interests for applications in tumor modeling and drug screening. − Microfluidics allows precise manipulation of fluidics in microchannels to recreate the tumor microenvironment with better-mimicked key parameters such as blood flow and gradient of biochemical cues, thereby allowing cell culture and drug screening in more physiological relevant conditions. , The incorporation of hydrogel scaffolds enables coculture of multiple cell types in a three-dimensional (3D) matrix . Moreover, the transparent microfluidic devices allow real-time assessment of cell–cell interaction and on-chip functional assays to characterize drug responses, which is particularly favorable for evaluating macrophage-based drug carriers. , Taken together, microfluidics is promising to provide reliable evaluation of macrophage-based carriers, including real-time assessment of macrophage migration toward tumors, intercellular crosstalk between macrophages and the tumor microenvironment, and subsequent drug responses.…”

Section: Introductionmentioning

confidence: 99%

Tumor-Microenvironment-on-a-Chip for Evaluating Nanoparticle-Loaded Macrophages for Drug Delivery

Wang

Liu

Wang

et al. 2020

ACS Biomater. Sci. Eng.

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Targeted drug delivery remains attractive but challenging for cancer therapy. Cell-mediated drug delivery has emerged as a promising strategy to improve targeted drug delivery to tumors due to the intrinsic ability of certain types of cells (e.g., macrophage) to pass through physiological barriers and specifically home to tumors. To fundamentally understand how macrophage-based drug carriers transport and interact with the tumor microenvironment, we developed a tumor-microenvironment-on-a-chip (TMOC) model that enables the coculture of tumor spheroids and macrophages in a three-dimensional (3D) gel matrix. By introducing drug-loaded macrophages, the TMOC model allows real-time observation of macrophage migration toward the tumor, infiltration into tumor spheroids, and subsequent response of tumor to drugs. Our results demonstrated the superior capability of macrophages migrating toward the tumor and infiltrate tumor spheroids. Drug loading in macrophages had minimum effect on their cell viability, and drug-carrying macrophages exhibited greater tumor cell cytotoxicity compared to their nanoparticle counterparts. Our work highlighted the great potential of macrophages as novel drug carriers for targeted drug delivery, and the TMOC model serves as a versatile platform to enable quick evaluation of such cell-mediated drug delivery systems.

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A numerical investigation of drug extravasation using a tumour–vasculature microfluidic device

Cited by 9 publications

References 19 publications

Axons-on-a-chip for mimicking non-disruptive diffuse axonal injury underlying traumatic brain injury

Axons-on-a-chip for mimicking non-disruptive diffuse axonal injury underlying traumatic brain injury

Axons-on-a-Chip for Mimicking Non-Disruptive Diffuse Axonal Injury underlying Traumatic Brain Injury

Tumor-Microenvironment-on-a-Chip for Evaluating Nanoparticle-Loaded Macrophages for Drug Delivery

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