A one-step sample pretreatment and loading method for complex sample separation with supercritical fluid chromatography

Dai, Yingping; Jiang, Dasen; Fu, Qing; Dai, Zhuoshun; Bai, Guoyu; He, Yubei; Han, Ruizhi; Liang, Xinmiao

doi:10.1016/j.supflu.2022.105516

Cited by 3 publications

(1 citation statement)

References 29 publications

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“…Although the separation could meet the requirements (less than 8 min), the peak shape was broadened or collapsed, especially the first peak (Figure 4A). The proportion of Sc-CO 2 in the starting mobile phase is relatively high, due to the great difference in elution strength and viscosity between alcohol and Sc-CO 2 , the sample is easy to precipitate in the column or pipeline during the injection process, especially in the case of large injection volume, causing the peak tailing, severe distortion of the peak shape and the column pressure to rise [27,28]. To solve this problem, n-hexane, which has similar polarity to Sc-CO 2 , was used as a dilution solvent.…”

Section: 21mentioning

confidence: 99%

Extraction and isolation of diphenylheptanes and flavonoids fromAlpinia officinarumHance using supercritical fluid extraction followed by supercritical fluid chromatography

Dai

et al. 2023

J of Separation Science

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In this paper, an off-line combination method of supercritical fluid extraction and supercritical fluid chromatography was developed for the selective extraction and isolation of diphenylheptanes and flavonoids from Alpinia officinarum Hance.The enrichment of target components was successfully achieved using supercritical fluid extraction with the following conditions (8% ethanol as co-solvent at 45 • C and 30 MPa for 30 min). Taking full advantage of the complementarity of supercritical fluid chromatography stationary phases, a two-step preparative supercritical fluid chromatography strategy was constructed. The extract was firstly divided into seven fractions on a Diol column (250 × 20 mm internal diameter, 10 μm) within 8 min by gradient elution increasing from 5% to 20% modifier (methanol) at 55 ml/min and 15 MPa. Then the seven fractions were separated by using a 1-AA or a DEA column (250 × 19 mm internal diameter, 5 μm) at 50 ml/min and 13.5 MPa. This two-step strategy showed superior separation ability for structural analogs. As a result, seven compounds, including four diphenylheptanes and three flavonoids with high purity, were successfully obtained. The developed method is also helpful for the extraction and isolation of other structural analogs of traditional Chinese medicines.

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Section: 21mentioning

confidence: 99%

Extraction and isolation of diphenylheptanes and flavonoids fromAlpinia officinarumHance using supercritical fluid extraction followed by supercritical fluid chromatography

Dai

et al. 2023

J of Separation Science

Self Cite

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Vacuum differential mobility spectrometry combined with column-switching liquid chromatography- mass spectrometry for the analysis of pyrrolizidine alkaloids in tea samples

Girard

Knight

Hopfgartner

2023

Journal of Chromatography A

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Insights on nano-scale flow and interactive transportation of hazardous pollutants under supercritical fluid environment

Ragui,

Chen

2024

Physics of Fluids

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This perspective paper aims to spark a fundamental debate on the key laws that would combine the nano- to micro-scale flow dynamics of resident contaminants in soil systems under supercritical phase-assisted transportation. The rank of supercritical solvents (SCFs) for hazardous polycyclic aromatic hydrocarbons, benzene, toluene, ethylbenzene, and xylenes is deeply discussed beside its confines to meet the current requests of in situ/ex situ fields. The scaling behaviors during soil–pollutants–SCFs interactions are highlighted to underline the hidden laws behind the basic transportation under flooding remediation. A new strategy based on artificial intelligence learning is emphasized with a demonstrative sample to predict the solubility behavior of this kind of contaminants during a spatiotemporal mechanism. This debate illustrates the conjugate experimental and numerical pathways to deal with the complex nano-remediation dynamics and sheds the light on the current trends and future outlook with techno-economic concerns to promote the faith of a clean and sustainable environment.

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A one-step sample pretreatment and loading method for complex sample separation with supercritical fluid chromatography

Cited by 3 publications

References 29 publications

Extraction and isolation of diphenylheptanes and flavonoids fromAlpinia officinarumHance using supercritical fluid extraction followed by supercritical fluid chromatography

Extraction and isolation of diphenylheptanes and flavonoids fromAlpinia officinarumHance using supercritical fluid extraction followed by supercritical fluid chromatography

Vacuum differential mobility spectrometry combined with column-switching liquid chromatography- mass spectrometry for the analysis of pyrrolizidine alkaloids in tea samples

Insights on nano-scale flow and interactive transportation of hazardous pollutants under supercritical fluid environment

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