A p37-based ELISA used to monitor anti–Mycoplasma hyorhinis IgG in serum from pigs immunized with inactivated M. hyorhinis vaccines

Abstract

Mycoplasma hyorhinis is an important pathogen of swine that can often occur as a respiratory coinfection with viral pathogens, but can also cause arthritis and polyserositis in infected animals. To date, no assay is available to assess the serologic response to M. hyorhinis vaccines, to our knowledge. We used recombinantly expressed M. hyorhinis p37 protein to monitor the magnitude of the IgG response in vaccinated animals. The assay was able to distinguish animals vaccinated with M. hyorhinis from those vaccinated with the other important Mycoplasma species: M. hyopneumoniae and M. hyosynoviae. When formulated with an ideal adjuvant, inactivated vaccines designed to protect animals against M. hyorhinis induced a measurable and dose-dependent antibody response against the p37 protein. Additionally, the protein appears to be highly conserved between strains of M. hyorhinis isolated in the United States. The specificity of the assay as well as the conservation and immunogenicity of the p37 protein make it an ideal candidate antigen for use in measuring the immune response against M. hyorhinis after vaccination in weaned pigs.

Co-infections by Mycoplasma hyopneumoniae, Mycoplasma hyorhinis and Mycoplasma flocculare in macroscopic lesions of lung consolidation of pigs at slaughter

“…Mhr infections are generally asymptomatic (Gomes Neto et al, 2012), with cases of pneumonia, arthritis, polyserosite and conjunctivitis having already been collected together with infection by this microorganism (Tacker and Minion, 2012), its role in lung lesions is not clear, however, the current study may clarify this role. According to Bumgardner et al (2018), Mhr, can often be present in respiratory co-infections with viral pathogens.…”

Identification of specific B cell linear epitopes of Mycoplasma hyorhinis P37 protein using monoclonal antibodies against baculovirus-expressed P37 protein

“…P37 is thought to function in adherence to host cells and has been used as an antigen to monitor the production of antibodies in animals immunized with inactivated Mhr vaccine [15,17,22]. In this study, we focused on the conserved P37 protein of Mhr as a potential target for diagnostic development.…”

Identification of specific B cell linear epitopes of mycoplasma hyorhinis P37 protein using monoclonal antibodies against baculovirus-expressed P37 protein

“…P37 is thought to function in adherence to host cells and has been used as an antigen to monitor the production of antibodies in animals immunized with inactivated Mhr vaccine [17, 19, 24]. In this study, we focused on the conserved P37 protein of Mhr as a potential target for diagnostic development.…”

“…Previously, the P37 protein was used as a coating antigen to measure the immunoglobulin G (IgG) responses in swine vaccinated with an inactivated Mhr vaccine [19]. However, it was unclear whether P37 protein could be used as an accurate indicator to identify naturally infected pigs in lung tissues, the role of P37 in the process of infection, or the precise epitope of P37.…”