A paper-based electrochemical immunosensor with reduced graphene oxide/thionine/gold nanoparticles nanocomposites modification for the detection of cancer antigen 125

Fan, Yingli; Shi, Shengyu; Ma, Jan; Guo, Yu

doi:10.1016/j.bios.2019.03.063

Cited by 149 publications

(60 citation statements)

References 37 publications

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“…Recent study, Fan et al [24] developed paper-based electrochemical immunosensor to detect cancer antigen 125 (CA125) by screen-printing method. The reduced nanocomposites of graphene oxide/thionine/gold (rGO/Thi/AuNPs) were compounded and coated for CA125 antibody (anti-CA125) immobilization and identification signal amplification on the paper electrode of the immunosensor.…”

Section: Cancer Antigen 125 (Ca125)mentioning

confidence: 99%

Current and Prospective of Breast Cancer Biomarkers

Benjamin¹,

Lima²

2021

Molecular Biotechnology

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Biomarkers have shown great promise over the past decade the process of drug development more effective and have become an integral part of diagnosis of diseases. Biosensors were integrated with biomarker detection and point-of-care detection for signal amplification, high specificity and sensitivity, rapid response time, low cost, simplicity and multi-analytical testing. In order to detect more sensitively, these particular biomarkers have been explored with the possibility of real-time measurements in order to develop simple and compact systems which can analyze complex specimens. Various biosensors including electrochemical biosensors have recently been developed based on disease-specific biomarkers in the diagnosis of cancer disease. The main objective of the book chapter is to review research with new materials/methods in electrochemical biosensing techniques to detection of breast cancer biomarkers and evaluating latest techniques for detection of important analytes in real samples. In this book chapter, the recent development of electrochemical biosensors of breast cancer biomarkers will be reviewed. Furthermore, recent and future trend application of breast cancer biomarkers will be discussed.

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Section: Cancer Antigen 125 (Ca125)mentioning

confidence: 99%

Current and Prospective of Breast Cancer Biomarkers

Benjamin¹,

Lima²

2021

Molecular Biotechnology

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“…Immunosensors combining immunochemical reactions based on the antibody (Ab)-antigen (Ag) recognition with an appropriate electrochemical transducer provide significant advantages for bioanalysis, including cost-effectiveness, low reagent and sample volume, portability, high-specificity and sensitivity, and high-throughput analysis [ 76 ]. Immunosensors have applications in various fields of analysis, such as clinical medicine [ 77 , 78 ], environmental pollutant evaluation [ 79 , 80 , 81 ], food inspection [ 82 , 83 ], and pathogenic microorganism detection [ 84 , 85 ]. The incorporation of MXenes in the fabrication of immunosensors provide opportunities for increasing bioactivity of immobilized Abs on electrode surfaces, increasing surface area, and improving detection sensitivity.…”

Section: Classes Of Bioanalytical Sensors Based On Mxenesmentioning

confidence: 99%

MXenes-Based Bioanalytical Sensors: Design, Characterization, and Applications

Khan

Andreescu

2020

Sensors

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MXenes are recently developed 2D layered nanomaterials that provide unique capabilities for bioanalytical applications. These include high metallic conductivity, large surface area, hydrophilicity, high ion transport properties, low diffusion barrier, biocompatibility, and ease of surface functionalization. MXenes are composed of transition metal carbides, nitrides, or carbonitrides and have a general formula Mn+1Xn, where M is an early transition metal while X is carbon and/or nitrogen. Due to their unique features, MXenes have attracted significant attention in fields such as clean energy production, electronics, fuel cells, supercapacitors, and catalysis. Their composition and layered structure make MXenes attractive for biosensing applications. The high conductivity allows these materials to be used in the design of electrochemical biosensors and the multilayered configuration makes them an efficient immobilization matrix for the retention of activity of the immobilized biomolecules. These properties are applicable to many biosensing systems and applications. This review describes the progress made on the use and application of MXenes in the development of electrochemical and optical biosensors and highlights future needs and opportunities in this field. In particular, opportunities for developing wearable sensors and systems with integrated biomolecule recognition are highlighted.

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“…SPEs on the surface of the paper were explored by several groups [ 151 ]. Fan et al fabricated a SPE on the surface of paper modified with graphene oxide/thionine/gold nanoparticles (rGo/Thi/AuNPs) for detection of CA 125 [ 152 ]. Using custom-designed screens, electrodes were patterned onto wax-printed paper.…”

Section: Screen Printingmentioning

confidence: 99%

“…The (rGo/Thi/AuNPs) were used for not only antibody immobilization but also signal amplification. The detection principle followed that the CA 125 antigen could decrease the current response of thionine, which corresponded to the concentration of CA 125 [ 152 ]. Using differential pulse voltammetry, the immunosensor displayed a linear range from 0.1 to 200 U mL −1 with LOD of 0.01 U mL −1 [ 152 ] ( Figure 12 ).…”

Section: Screen Printingmentioning

confidence: 99%

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Printed Electrodes in Microfluidic Arrays for Cancer Biomarker Protein Detection

et al. 2020

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Medical diagnostics is trending towards a more personalized future approach in which multiple tests can be digitized into patient records. In cancer diagnostics, patients can be tested for individual protein and genomic biomarkers that detect cancers at very early stages and also be used to monitor cancer progression or remission during therapy. These data can then be incorporated into patient records that could be easily accessed on a cell phone by a health care professional or the patients themselves on demand. Data on protein biomarkers have a large potential to be measured in point-of-care devices, particularly diagnostic panels that could provide a continually updated, personalized record of a disease like cancer. Electrochemical immunoassays have been popular among protein detection methods due to their inherent high sensitivity and ease of coupling with screen-printed and inkjet-printed electrodes. Integrated chips featuring these kinds of electrodes can be built at low cost and designed for ease of automation. Enzyme-linked immunosorbent assay (ELISA) features are adopted in most of these ultrasensitive detection systems, with microfluidics allowing easy manipulation and good fluid dynamics to deliver reagents and detect the desired proteins. Several of these ultrasensitive systems have detected biomarker panels ranging from four to eight proteins, which in many cases when a specific cancer is suspected may be sufficient. However, a grand challenge lies in engineering microfluidic-printed electrode devices for the simultaneous detection of larger protein panels (e.g., 50–100) that could be used to test for many types of cancers, as well as other diseases for truly personalized care.

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A paper-based electrochemical immunosensor with reduced graphene oxide/thionine/gold nanoparticles nanocomposites modification for the detection of cancer antigen 125

Cited by 149 publications

References 37 publications

Current and Prospective of Breast Cancer Biomarkers

Current and Prospective of Breast Cancer Biomarkers

MXenes-Based Bioanalytical Sensors: Design, Characterization, and Applications

Printed Electrodes in Microfluidic Arrays for Cancer Biomarker Protein Detection

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