A partial hepatectomy results in altered expression of clock-related and cyclic glyceraldehyde 3-phosphate dehydrogenase (GAPDH) genes

Iwasaki, Takuya; Nakahama, Ken-ichi; Nagano, Mamoru; Fujioka, Atsuko; Ohyanagi, Harumasa; Shigeyoshi, Yasufumi

doi:10.1016/j.lfs.2003.11.010

Cited by 8 publications

(6 citation statements)

References 23 publications

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“…2.2.1 (Applied Biosystems), and values for target genes were corrected for endogenous control (GAPDH). A previous report suggested that GAPDH is significantly regulated by hepatectomy (Iwasaki et al, 2004); however, when GAPDH levels were presented in graphical format, no significant alterations in response to PB or hepatectomy were observed (data not shown).…”

Section: Methodsmentioning

confidence: 71%

The Nuclear Receptor Constitutively Active/Androstane Receptor Regulates Type 1 Deiodinase and Thyroid Hormone Activity in the Regenerating Mouse Liver

Tien¹,

Matsui²,

Moore³

et al. 2007

The Journal of Pharmacology and Experimental Therapeutics

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We observed that the level of reverse triiodothyronine (rT3) was significantly increased after partial hepatectomy (PH) in both wild-type and constitutively active/androstane receptor (CAR) knockout (KO) mice, and treatment with phenobarbital (PB), a CAR activator, after PH decreased rT3 to restore its original level only in wild-type mice. On the other hand, no significant changes in the level of total T3 or free T3 in the serum were observed in either wild-type or CAR KO mice after PH or treatment with PB. Type 1 deiodinase (D1) activity and expression were significantly reduced by PH and up-regulated by PB in a CAR-dependent manner. In addition, known T3-regulated genes [tyrosine aminotransferase (TAT) and basic transcription element binding protein (BTEB)] were also significantly decreased by PH and induced by PB. Injection of rT3 into normal mice revealed that rT3 is capable of repressing the known thyroid hormone-regulated genes Tat, Bteb, and Cpt-1 in the liver. Our results suggest that PH decreases D1 activity leading to increased rT3 level, resulting in the repression of T3 target genes. Subsequent treatment with PB decreases rT3 in a CARdependent manner through the up-regulation of the D1 gene.

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Section: Methodsmentioning

confidence: 71%

The Nuclear Receptor Constitutively Active/Androstane Receptor Regulates Type 1 Deiodinase and Thyroid Hormone Activity in the Regenerating Mouse Liver

Tien¹,

Matsui²,

Moore³

et al. 2007

The Journal of Pharmacology and Experimental Therapeutics

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“…These experiments have revealed that NK cells show clock gene daily oscillations in vivo in both light-entrained rats [73] and in rats maintained in constant darkness [74]. The characteristics of clock transcript oscillations in enriched NK cells are similar to those seen in other peripheral tissues: Per1 transcript peaks at the time of the light-dark transition [90][91][92]. So does Per2, with a longer acrophase than Per1 [90,93].…”

Section: The Circadian Molecular Clock In Nk Cells and Other Leukocytesmentioning

confidence: 78%

Are Circadian Rhythms the Code of Hypothalamic-Immune Communication? Insights from Natural Killer Cells

Arjona

Sarkar

2007

Neurochem Res

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Circadian rhythms in physiology and behavior are ultimately regulated at the hypothalamic level by the suprachiasmatic nuclei (SCN). This central oscillator transduces photic information to the cellular clocks in the periphery through the autonomic nervous system and the neuroendocrine system. The fact that these two systems have been shown to modulate leukocyte physiology supports the concept that the circadian component is an important aspect of hypothalamic-immune communication. Circadian disruption has been linked to immune dysregulation, and recent reports suggest that several circadian clock genes, in addition to their time-keeping role, are involved in the immune response. In this overview, we summarize the findings demonstrating that Natural Killer (NK) cell function is under circadian control.

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“…In this study we demonstrate for the first time the expression of canonical components of the cellular clock in NK cells as well as their circadian oscillations in NK cells enriched from the rat spleen. Moreover, clock gene transcripts oscillate in enriched NK cells with many properties similar to those seen in other peripheral tissues: Per1 transcript peaks at the time of the light-dark transition (5,35,36). So does Per2, with a longer acrophase than Per1 (5, 10).…”

Section: Discussionmentioning

confidence: 97%

Circadian Oscillations of Clock Genes, Cytolytic Factors, and Cytokines in Rat NK Cells

Arjona

Sarkar

2005

The Journal of Immunology

192

141

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A growing body of knowledge is revealing the critical role of circadian physiology in the development of specific pathological entities such as cancer. NK cell function participates in the immune response against infection and malignancy. We have reported previously the existence of a physiological circadian rhythm of NK cell cytolytic activity in rats, suggesting the existence of circadian mechanisms subjacent to NK cell function. At the cellular level, circadian rhythms are originated by the sustained transcriptional-translational oscillation of clock genes that form the cellular clock apparatus. Our aim in this study was to investigate the presence of molecular clock mechanisms in NK cells as well as the circadian expression of critical factors involved in NK cell function. For that purpose, we measured the circadian changes in the expression of clock genes (Per1, Per2, Bmal1, Clock), Dbp (a clock-controlled output gene), CREB (involved in clock signaling), cytolytic factors (granzyme B and perforin), and cytokines (IFN-γ and TNF-α) in NK cells enriched from the rat spleen. The results obtained from this study demonstrate for the first time the existence of functional molecular clock mechanisms in NK cells. Moreover, the circadian expression of cytolytic factors and cytokines in NK cells reported in this study emphasizes the circadian nature of NK cell function.

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A partial hepatectomy results in altered expression of clock-related and cyclic glyceraldehyde 3-phosphate dehydrogenase (GAPDH) genes

Cited by 8 publications

References 23 publications

The Nuclear Receptor Constitutively Active/Androstane Receptor Regulates Type 1 Deiodinase and Thyroid Hormone Activity in the Regenerating Mouse Liver

The Nuclear Receptor Constitutively Active/Androstane Receptor Regulates Type 1 Deiodinase and Thyroid Hormone Activity in the Regenerating Mouse Liver

Are Circadian Rhythms the Code of Hypothalamic-Immune Communication? Insights from Natural Killer Cells

Circadian Oscillations of Clock Genes, Cytolytic Factors, and Cytokines in Rat NK Cells

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