A passenger strand variant in miR-196a2 contributes to asthma severity in children and adolescents: A preliminary study

Hussein, Mohammad; Toraih, Eman A.; Aly, Nagwa M.; Riad, Eman; Fawzy, Manal S.

doi:10.1139/bcb-2016-0010

Cited by 15 publications

(12 citation statements)

References 45 publications

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“…30 Some study in Egyptian population proposed that mir196a2 rs11614913 polymorphism could be associated with asthma severity. 31 Some of reports showed that polymorphism of mir196a2 rs11614913, as a biomarker associated with some types of cancer such as gallbladder, liver, breast, gastric, prostate, as well as autoimmune disease. 25 Based on the fact that genetic variants can alter the expression and function of miRNAs, we carried out an association study between miRNA polymorphism and MS susceptibility.…”

Section: Introductionmentioning

confidence: 99%

Genetic Variation in Intergenic and Exonic miRNA Sequence and Risk of Multiple Sclerosis in the Isfahan Patients

Golshani

Hojati

Sharifzadeh

et al. 2018

IJAAI

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MicroRNAs (miRNAs), have been documented to perform a key role in the pathogenesis of multiple sclerosis (MS), a chronic inflammatory and autoimmune disease. Recent studies have shown that single nucleotide polymorphism in the sequence of the miRNA may change their production and expression which can lead to miRNA dysfunction and pathogenicity. Some studies have reported the relationship between miRNA polymorphism and the increased risk of autoimmune disease. This study was conducted to investigate the association between mir155 rs767649, mir196a2 rs11614913 and mir23a rs3745453 polymorphism and the risk of multiple sclerosis in the Iranian MS patients in Isfahan. A population of 80 patients and the same number control were selected. After DNA extraction, genotyping was performed through tetra amplification refractory mutation system-PCR method (T ARMS PCR). The frequencies of TT, TC and CC genotypes of mir23a were 46, 35 and 20% in MS patients and 42, 14 and 24 in healthy subjects respectively. These results showed that individuals carrying the genotypes of rs3745453 TC had a 2.3-fold increased risk of MS (OR=2.3, p=0.048). There was no significant difference between genotypes and allele frequency of mir155 and mir196a2 in patients and healthy controls (p>0.05). Our findings specified that CT heterozygosity in mir23a gene significantly related with risk of MS. Unlike mir155 and mir196a2, mir23a rs3745453 may have contributed to the etiology of MS in Isfahan patients. However, extensive studies are required to gain more reliable and authentic results.

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Section: Introductionmentioning

confidence: 99%

Genetic Variation in Intergenic and Exonic miRNA Sequence and Risk of Multiple Sclerosis in the Isfahan Patients

Golshani

Hojati

Sharifzadeh

et al. 2018

IJAAI

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“…Patients were diagnosed according to the Global Initiative for Asthma (GINA) guidelines . Clinical examination was done for determining severity of the disease, therapeutic history and comorbidities . Asthmatic patients with chronic comorbidities, with manifestations of acute respiratory infection within 3 weeks, on systemic steroids within 2 weeks, or non‐compliant to treatment were excluded from the study.…”

Section: Methodsmentioning

confidence: 99%

“…PCRs were carried out in a 25‐μL reaction volume containing 12.5 μL 2 × Taqman ® genotyping Master Mix and 1.25 μL TaqMan ® SNP Genotyping Assay Mix (Thermo Fisher Scientific) with 40 ng genomic DNA. Real‐time PCR amplification was run on StepOne ™ Real‐Time PCR (Applied Biosystems) as previously described in details in our prior publication . Quality control measures included blinded analyses and replicates of 10% of samples with 100% concordance.…”

Section: Methodsmentioning

confidence: 99%

Association of group‐specific component exon 11 polymorphisms with bronchial asthma in children and adolescents

et al. 2019

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Several studies have investigated the association of Group-specific Component (GC) gene, also known as vitamin D-binding protein (VDBP), and various respiratory disorder susceptibility with conflicting results. In this sense, we aimed to investigate whether rs7041 and rs4588 variants confer susceptibility to bronchial asthma in a sample of an Egyptian population and to elucidate by in silico analysis the structural and functional impact of these variants. Group-specific Component polymorphisms rs7041 and rs4588 were genotyped in 192 Egyptian children and adolescents (96 with asthma and 96 healthy controls) by TaqMan single nucleotide polymorphism genotyping assay. The rs7041 GG genotype showed a significantly elevated frequency among patients under codominant, dominant, recessive and allelic models where the patient group had greater carriage rate of G allele [OR 2.15, CI 95% (1.32-3.50; P = 0.002)], while rs4588 CA and AA genotypes were found to be protective genotypes with controls showing a greater carriage rate of A allele [OR 0.52, CI 95% (0.30 -0.90; P = 0.02)]. Three haplotype allele combinations were identified with frequencies of GC (44.3%), TC (31.3%) and TA (24.5%) in the total study population. GC haplotype was shown to be more frequent in controls, while TC and TA haplotypes were more predominant in the patient group. Only rs7041 variant showed a significant association with family history and pubertal status. In conclusion, both study GC variants could be implicated in childhood bronchial asthma pathogenesis; rs7041 GG genotype and G allele increased asthma risk while rs4588 AA genotype and A allele conferred protection in the study population.

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“…Bronchial asthma is a chronic heterogeneous respiratory disease that is characterized by airway inflammation, recurring bronchial obstruction, and airway hyper-responsiveness [ 1 , 2 ]. Most common histopathological features are inflammatory cell infiltration, sub-basement fibrosis, smooth muscle hypertrophy, mucus hypersecretion, injury to epithelial cells, and angiogenesis [ 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

“…Treatment with anti-inflammatory drugs or bronchodilators usually improves some of these features. Nevertheless, therapeutic response relies on the interplay between environmental exposure and genetic background [ 2 ]. Despite several advances over the past decades in understanding the underlying mechanisms involved in the disease, there are no current satisfactory strategies for the cure or prevention of long-term decline in pulmonary function [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Structure and functional impact of seed region variant in MIR-499 gene family in bronchial asthma

Toraih¹,

Hussein²,

Ageeli³

et al. 2017

Respir Res

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BackgroundSmall non-coding RNAs (microRNAs) have been evolved to master numerous cellular processes. Genetic variants within microRNA seed region might influence microRNA biogenesis and function. The study aimed at determining the role of microRNA-499 (MIR-499) gene family polymorphism as a marker for susceptibility and progression of bronchial asthma and to analyze the structural and functional impact of rs3746444 within the seed region.MethodsGenotyping for 192 participants (96 patients and 96 controls) in the discovery phase and 319 subjects (115 patients and 204 controls) in the replication phase was performed via Real Time-Polymerase Chain Reaction technology. Patients underwent the methacholine challenge test and biochemical analysis. Gene structural and functional analysis, target prediction, annotation clustering, and pathway enrichment analysis were executed. Predicted functional effect of rs37464443 SNP was analyzed.ResultsmiR-499 gene family is highly implicated in inflammation-related signaling pathways. Rs374644 (A > G) in MIR499A and MIR499B within the seed region could disrupt target genes and create new genes. The G variant was associated with high risk of developing asthma under all genetic association models (G versus A: OR = 3.27, 95% CI = 2.53–4.22; GG versus AA: OR = 9.52, 95% CI = 5.61–16.5; AG versus AA: OR = 2.13, 95% CI = 1.24–3.46; GG + AG versus AA: OR = 4.43, 95% CI = 2.88–6.82). GG genotype was associated with poor pre-bronchodilator FEV1 (p = 0.047) and the worst bronchodilator response after Salbutamol inhalation, represented in low peaked expiratory flow rate (p = 0.035).ConclusionsmiR-499 rs3746444 (A > G) polymorphism was associated with asthma susceptibility and bronchodilator response in Egyptian children and adolescents. Further functional analysis is warranted to develop more specific theranostic agents for selecting targeted therapy.Electronic supplementary materialThe online version of this article (10.1186/s12931-017-0648-0) contains supplementary material, which is available to authorized users.

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A passenger strand variant in miR-196a2 contributes to asthma severity in children and adolescents: A preliminary study

Cited by 15 publications

References 45 publications

Genetic Variation in Intergenic and Exonic miRNA Sequence and Risk of Multiple Sclerosis in the Isfahan Patients

Genetic Variation in Intergenic and Exonic miRNA Sequence and Risk of Multiple Sclerosis in the Isfahan Patients

Association of group‐specific component exon 11 polymorphisms with bronchial asthma in children and adolescents

Structure and functional impact of seed region variant in MIR-499 gene family in bronchial asthma

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