A PCR-based lateral flow assay for the detection of Turkey ingredient in food products

Rapid point‐of‐care testing methods/devices for meat species identification: A review

“…These types of systems are called micro total analysis systems (μTAS). However, in some applications, such as in meat species identification, sample preprocessing is carried out before the detection process (Qin et al., 2019; Zhao, Wang, et al., 2020). Most of the POC devices that have been developed for meat species identification are based on detection of analytes of lateral flow‐based platforms.…”

“…Mostly, biotin and fluorescence isothiocyanate (FITC) have been used as labels, and gold nanoparticles (AuNPs) have been used as detection tag (Figure 1). Streptavidin (SA) protein forms strong noncovalent bonds with biotin; hence, it is conjugated with AuNPs (for conjugation) or immobilized at T‐line (for recognition) (Zhao, Wang, et al., 2020). Some antibody‐independent NA‐LFA methods have also been developed in which biotinylated probes or amplicons have been used with SA or poly(dT) sequence as a binding molecule (Kortli et al., 2020; Magiati, Myridaki, Christopoulos, & Kalogianni, 2019; Ngom, Guo, Wang, & Bi, 2010).…”

“…Some antibody‐independent NA‐LFA methods have also been developed in which biotinylated probes or amplicons have been used with SA or poly(dT) sequence as a binding molecule (Kortli et al., 2020; Magiati, Myridaki, Christopoulos, & Kalogianni, 2019; Ngom, Guo, Wang, & Bi, 2010). A number of NA‐LFAs or NA‐LFS or NA‐LFD devices have been developed for POC detection of animal species in different meat products (Figure 1) (Li et al., 2019; Yan et al., 2020; Zhang, Zhang, et al., 2019; Zhao, Wang, et al., 2020; Zheng et al., 2020).…”

“…A simple PCR‐based LFA was developed by Zhao, Wang, et al. (2020) for the identification of turkey ingredients in foods. During PCR reaction, the amplicons were labeled with FITC and biotin using labeled species‐specific primers.…”

“…These methods have coupled two or more techniques for detection purpose. The methods based on PCR, CPA, SEA, LAMP, and RPA reactions have been coupled with lateral flow or microfluidic devices and strips (Li et al., 2019; Wang, Yan, et al., 2019; Wang, Wang, Shi, Ma, & Chen, 2020; Zhang, Zhang, et al., 2019; Zhao, Wang, et al., 2020; Zheng et al., 2020). Other simpler procedures such as fast nucleic acid extraction and its direct application onto the detection platform have also been proposed to complete the whole detection in a lesser time duration (Kuswandi, Gani, & Ahmad, 2017; Liu et al., 2019; Shi et al., 2017).…”