A Perspective on the Molecular Identification, Classification, and Epidemiology of Enterocytozoon bieneusi of Animals

Koehler, Anson V.; Zhang, Yan; Gasser, Robin B.

doi:10.1007/978-3-030-93306-7_14

Cited by 11 publications

(23 citation statements)

References 181 publications

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“…CEbC, NCF7, Peru 9, PigEBITS9, PtEb VI, SHW1, WL8) and EbpC (syn. CHG23, E, Peru 4, SC03, WL13, WL17) were the most common genotypes either in humans or in animals worldwide [15]. To date, genotype D has been identified in 91 host species distributed in 40 countries, while genotype EbpC has been identified in 43 host species in 15 countries.…”

Section: T C T G a T G T A C T G T T G T C G C T Gmentioning

confidence: 99%

“…With accumulation of genotyping data of E. bieneusi, there has been a huge number of genotypes identified. By June 2021, 819 genotypes had been documented, with 465 in China [15], revealing the vast genetic variation of E. bieneusi in the ITS region of the rRNA gene. In the present study, 11 novel genotypes of E. bieneusi were obtained, and all of them fell into zoonotic group 1.…”

Section: T C T G a T G T A C T G T T G T C G C T Gmentioning

confidence: 99%

“…Enterocytozoon bieneusi is also infrequently identified in other epithelial cells of patients with AIDS, i.e., in the biliary tree, gallbladder, nonparenchymal liver cells, pancreatic duct, and tracheal, bronchial, and nasal epithelia [37]. In addition to humans, E. bieneusi has also been detected in at least 210 animal species, suggesting its zoonotic potential [15]. The findings of the same E. bieneusi genotypes in both humans and animals support the presumption of zoonotic potential.…”

Section: Introductionmentioning

confidence: 99%

“…Enterocytozoon bieneusi is now known as a species complex. By analyzing sequences of the internal transcribed spacer (ITS) region of the ribosomal RNA (rRNA) gene with a high degree of genetic polymorphism, 819 genotypes had been identified by June 2021: 126 exclusively in humans, 614 exclusively in animals, 58 in both humans and animals, and 21 only in environmental samples [15]. These genotypes have been divided into 11 different phylogenetic groups and an outlier with a varying degree of host specificity and zoonotic potential [17].…”

Section: Introductionmentioning

confidence: 99%

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Occurrence and genetic characterization of Enterocytozoon bieneusi in pet dogs in Yunnan Province, China

Jian,

Zi,

Wang

et al. 2024

Parasite

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Enterocytozoon bieneusi is the most common microsporidian species in humans and can affect over 200 animal species. Considering possible increasing risk of human E. bieneusi infection due to close contact with pet dogs and identification of zoonotic E. bieneusi genotypes, 589 fresh fecal specimens of pet dogs were collected from Yunnan Province, China to determine the occurrence of E. bieneusi, characterize dog-derived E. bieneusi isolates, and assess their zoonotic potential at the genotype level. Enterocytozoon bieneusi was identified and genotyped by PCR and sequencing of the internal transcribed spacer (ITS) region of the ribosomal RNA (rRNA) gene. Twenty-nine specimens (4.9%) were positive. A statistical difference was observed in occurrence rates of E. bieneusi in pet dogs among 11 sampling sites by Fisher’s exact test. Fifteen genotypes were identified and all of them phylogenetically belonged to zoonotic group 1, including four known genotypes (EbpC, D, Peru 8, and Henan-III) and 11 novel genotypes. Genotype Henan-III was reported in dogs for the first time. The finding of known genotypes found previously in humans and novel genotypes falling into zoonotic group 1 indicates that dogs may play a role in the transmission of E. bieneusi to humans in the investigated areas.

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Section: T C T G a T G T A C T G T T G T C G C T Gmentioning

confidence: 99%

Section: T C T G a T G T A C T G T T G T C G C T Gmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Occurrence and genetic characterization of Enterocytozoon bieneusi in pet dogs in Yunnan Province, China

Jian,

Zi,

Wang

et al. 2024

Parasite

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show abstract

“…This tool has enhanced our understanding of the distribution and zoonotic potential of E. bieneusi genotypes in humans and various animals. Over 850 distinct genotypes of E. bieneusi have been identified in humans and animals, and this number is still increasing [12]. At least 126 of these genotypes have been recorded exclusively in humans, while 58 of them were zoonotic (present in both humans and animals) [12].…”

Section: Introductionmentioning

confidence: 99%

Enterocytozoon bieneusi in Wild Rats and Shrews from Zhejiang Province, China: Occurrence, Genetic Characterization, and Potential for Zoonotic Transmission

Zhang,

Yu,

et al. 2024

Microorganisms

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Globally, Enterocytozoon bieneusi has been detected in humans and various animal hosts. Wild rats and shrews have the potential to act as carriers of E. bieneusi, facilitating the parasite’s transmission to humans and domestic animals. We aimed to investigate the prevalence of E. bieneusi in 652 wild rats and shrews from Zhejiang Province, China, by amplifying the internal transcribed spacer (ITS) region of rDNA through polymerase chain reaction (PCR). To determine animal species, we amplified the Cytochrome b (Cyt-b) gene in their fecal DNA using PCR. Furthermore, we determined the genotype of E. bieneusi by amplifying the ITS region of rDNA through PCR. Genetic traits and zoonotic potential were evaluated using similarity and phylogenetic analyses. Suncus murinus (n = 282) and five rat species, Rattus losea (n = 18), Apodemus agrarius (n = 36), Rattus tanezumi (n = 86), Rattus norvegicus (n = 155), and Niviventer niviventer (n = 75), were identified. The average infection rate of E. bieneusi was 14.1% (92/652) with 18.1% (51/282) in S. murinus and 11.1% (41/370) in rats (27.8% in R. losea, 22.2% in A. agrarius, 10.5% in R. tanezumi, 8.4% in R. norvegicus, and 8.0% in N. niviventer). Thirty-three genotypes were identified, including 16 known genotypes. The most commonly known genotypes were HNR-VI (n = 47) and Peru11 (n = 6). Type IV, KIN-1, SHW7, and HNPL-II were each found in two samples, while Macaque4, CH5, K, Henan-III, Henan-V, HNP-II, HNPL-I, HNPL-III, HNHZ-II, and HNHZ-III were each found in one sample. Additionally, 17 novel genotypes were discovered: WZR-VIII (n = 5), WZR-I to WZR-VII, WZR-IX to WZR-XII, and WZSH-I to WZSH-V (n = 1 each). Those 33 genotypes were divided into three groups: Group 1 (n = 25), Group 2 (n = 3), and Group 13 (n = 5). The initial report underscores the extensive occurrence and notable genetic diversity of E. bieneusi in wild rats and shrews from Zhejiang province, China. These results suggest that these animals play a pivotal role in the transmission of E. bieneusi. Furthermore, animals carrying the zoonotic genotypes of E. bieneusi pose a serious threat to residents.

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Molecular characterization of Enterocytozoon bieneusi genotypes in wild Altai marmot (Marmota baibacina) in Xinjiang, China: host specificity and adaptation

Tuo,

Zhang,

et al. 2023

Parasitol Res

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A Perspective on the Molecular Identification, Classification, and Epidemiology of Enterocytozoon bieneusi of Animals

Cited by 11 publications

References 181 publications

Occurrence and genetic characterization of Enterocytozoon bieneusi in pet dogs in Yunnan Province, China

Occurrence and genetic characterization of Enterocytozoon bieneusi in pet dogs in Yunnan Province, China

Enterocytozoon bieneusi in Wild Rats and Shrews from Zhejiang Province, China: Occurrence, Genetic Characterization, and Potential for Zoonotic Transmission

Molecular characterization of Enterocytozoon bieneusi genotypes in wild Altai marmot (Marmota baibacina) in Xinjiang, China: host specificity and adaptation

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