A Perspective on the Use of Fluorescent Imaging to Reveal Mechanisms of Breakup

King‐Smith, P. Ewen; Begley, Carolyn G.; Braun, Richard

doi:10.1080/02713683.2022.2098981

Current Eye Research

2022

DOI: 10.1080/02713683.2022.2098981

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A Perspective on the Use of Fluorescent Imaging to Reveal Mechanisms of Breakup

P. Ewen King‐Smith

Carolyn G. Begley

Richard Braun

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2024

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Cited by 2 publications

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“…An alternative “evaporation” model was proposed based on evidence that the TFLL is a barrier to evaporation, 6 like the skin. The skin lipid layer contains tightly stacked lamellae.…”

mentioning

confidence: 99%

High Resolution Images of Human Meibum Spread on Saline

King-Smith,

Begley,

Braun

2024

Invest. Ophthalmol. Vis. Sci.

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Purpose Understanding of the role of the tear film lipid layer (TFLL) in evaporative dry eye requires knowledge of its structure. X-ray studies show 11.1-nm thick lamellae in meibum at tear film temperature (approximately 35°C), whereas below 30°C, 4.88-nm thick lamellae predominate. Here, high resolution microscopy of meibum spread on saline is studied as a function of temperature, to compare with x-ray results. Methods A purpose-built high resolution color microscope, previously used to study the TFLL, was used to study meibum from 10 subjects. It was spread on buffered saline at near 40°C, and allowed to cool to room temperature. Analytical methods from previous studies were applied to measure meibum and lamellar thickness. Results Initially, an irregular “island” was formed, surrounded by a “background layer” of 7.8 ± 0.3 nm thickness. Dewetting of the meibum layer always occurred, leading to the formation of lens-shaped droplets. Below 30°C, the lenses start to emit “tails” having a multilamellar structure containing up to about 49 lamellae superimposed on the background layer, each lamella being 4.82 ± 0.13 nm thick. Conclusions Below 30°C, meibum spread on saline shows a multilamellar structure like the 4.88 nm thickness in x-ray studies, demonstrating the ability to observe and measure tightly stacked lamellae. In contrast, above 30°C, the 11.1 nm lamellae were not observed as in x-ray studies, indicating that these lamellae were not tightly stacked but may be separated by disordered lipid. The role of these findings in evaporative dry eye is discussed.

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“…An alternative “evaporation” model was proposed based on evidence that the TFLL is a barrier to evaporation, 6 like the skin. The skin lipid layer contains tightly stacked lamellae.…”

mentioning

confidence: 99%

High Resolution Images of Human Meibum Spread on Saline

King-Smith,

Begley,

Braun

2024

Invest. Ophthalmol. Vis. Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

A machine learning approach to predicting dry eye-related signs, symptoms and diagnoses from meibography images

Graham,

Kothapalli,

Wang

et al. 2024

Heliyon

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A Perspective on the Use of Fluorescent Imaging to Reveal Mechanisms of Breakup

Cited by 2 publications

References 41 publications

High Resolution Images of Human Meibum Spread on Saline

High Resolution Images of Human Meibum Spread on Saline

A machine learning approach to predicting dry eye-related signs, symptoms and diagnoses from meibography images

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