A PET-based fluorescent probe for monitoring labile Fe(ii) pools in macrophage activations and ferroptosis

Abstract

A fluorescent probe (COU-LIP-1) for monitoring labile Fe(II) pools (LIP) with high selectivity and sensitivity was developed utilizing coumarin 343 as the fluorophore and 3-nitrophenylazanyl ester as both the reactive...

Recent Advances of Fluorescence Probes for Imaging of Ferroptosis Process

“…Finally, imaging with Ac−H−FluNox demonstrated that the labile heme level was upregulated upon the induction of ferroptosis by erastin in HT−1080 cells. Similarly, Xing et al [45] reported another selective reaction−based probe COU−LIP−1 by the Fe 2+ −triggered N−O bond cleavage and applied it for monitoring intracellular labile Fe 2+ pools (Figure 5). COU−LIP−1 was designed by utilizing coumarin 343 as the fluorophore and 3−nitrophenylazanyl ester as both the recognition group and the fluorescence quenching group.…”

Recent advances in small-molecule fluorescent probes for studying ferroptosis

“…16). 140 In this probe, coumarin 343 was used as skeleton, and the 3-nitrophenylazanyl ester simultaneously served as Fe 2+ reactive site and quenching moiety. Based on the PET mechanism, probe 20 emitted faint fluorescence emission due to the quenching effect of 3-nitrophenylazanyl ester.…”

Versatile Switchable Targeted Polysiloxanes for High-Resolution Visualization of Mitochondrial and Lysosomal Interactions during Ferroptosis

“…The appearance of the peak at 7.85 ppm, 7.78 ppm (−CH 2 −NH−), and 3.60 ppm (−CH 2 −NH−) in 1 H NMR and the peaks at 132 and 38.2 ppm suggested the successful reaction of the carboxyl-containing triphenylphosphine with the amino group. The appearance of the peak at 3.80 ppm (−CH 2 −N) in 1 H NMR and 50.52 ppm (−CH 2 −N) in 13 C NMR suggested the successful reaction of the naphthalimide with the amino group. The above NMR information proved that Si-Mito-ONOO was successfully synthesized.…”

“…We characterized by using 1 H NMR and 13 C NMR. The appearance of the peak at 3.72 ppm (−CH 2 −N) in 1 H NMR and 48.15 ppm in 13 C NMR implied that Si-Lyso-ONOO was successfully synthesized. The appearance of the peak at 7.85 ppm, 7.78 ppm (−CH 2 −NH−), and 3.60 ppm (−CH 2 −NH−) in 1 H NMR and the peaks at 132 and 38.2 ppm suggested the successful reaction of the carboxyl-containing triphenylphosphine with the amino group.…”

“…NMR (400 MHz, CDCl 3 ) δ 8.63 (dd, J = 12.9, 6.5 Hz, 1H), 8.35 (d, J = 7.2 Hz, 1H), 8.29 (d, J = 8.3 Hz, 1H), 7.85 (dd, J = 12.2, 7.2 Hz, 1H), 7.78 (dd, J = 9.5, 5.1 Hz, 1H), 3.80 (d, J = 14.0 Hz, 1H), 3.60 (t, J = 14.4 Hz, 1H), 2.82 (dd, J = 19.9, 12.1 Hz, 1H), 2.50 (t, J = 4.9 Hz, 1H), 1.51 (s, 1H), 0.80−0.45 (m, 1H), 0.36−0.12 (m, 1H) 13. C NMR (101 MHz, CDCl 3 ) δ 179.41 (s), 159.31 (s), 142.36−124.24 (m), 119.95 (d, J = 96.2 Hz), 86.18 (s), 56.03−46.40 (m), 22.96 (dd, J = 583.0, 455.3 Hz), 2.13 (d, J = 187.8 Hz).Cell Imaging Experiments.…”