A pH-Modulated, Self-Replicating Peptide

Yao, Shao Q.; Ghosh, Indraneel; Zutshi, Reena; Chmielewski, Jean

doi:10.1021/ja9710619

Cited by 119 publications

(92 citation statements)

References 30 publications

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“…The ''minimal systems'' for self-replication developed by von Kiedrowski (37,38) and others for nucleic acids (39), peptides (40,41), small organic molecules (42), and ribozymes (43) all represent autocatalytic systems based on core templates larger than those in the Soai reactions. However, none of those systems involve prochiral molecules, meaning that the origin of the first imbalance in asymmetric centers is not at issue in these cases; when we examine von Kiedrowski's (37,38) autocatalytic system based on DNA hexamers, for example, we know we are already firmly committed and residing in the camp of the L-amino acids and the D-sugars.…”

Section: Discussionmentioning

confidence: 99%

Asymmetric Autocatalysis and Its Implications for the Origin of Homochirality

Blackmond

2004

ChemInform

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An autocatalytic reaction in which the reaction product serves as a catalyst to produce more of itself and to suppress production of its enantiomer serves as a mechanistic model for the evolution of homochirality. The Soai reaction provided experimental confirmation of this concept, nearly 50 years after it was first proposed. This Perspective offers a rationalization of the Soai autocatalytic reaction; accounting for enantiomeric excess and rate observations, that is both simple as well as gratifying in its implications for the chemical origin of life.

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Section: Discussionmentioning

confidence: 99%

Asymmetric Autocatalysis and Its Implications for the Origin of Homochirality

Blackmond

2004

ChemInform

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“…These reactions proceed in an autocatalytic manner if the new copies of T can direct the joining of A and B. The autocatalytic replication of the template molecule has been demonstrated for activated oligonucleotides [2][3][4], peptides [5,6], and small organic compounds [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

RETRACTED: Replication of an RNA ligase ribozyme under alternating temperature condition

Kim

Choi

et al. 2007

FEBS Letters

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Self-replication process of the RNA ligase ribozyme molecules was investigated by using the modified RNA ligase ribozyme under alternating temperature condition that enhances turnover rate of the RNA ligation reaction. In our experiment, the RNA ligase ribozyme system mainly undergoes a cross-catalytic replication process, in which two ribozymes catalyze each other's synthesis from a total of four RNA substrates under alternating temperature condition, resulting in time-dependent accumulation of additional copies of the starting ribozymes in a reaction mixture. The present study demonstrates that crosscatalytic replication in nucleic acids system can be efficiently devised under the alternating temperature condition.

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“…The self-replicating systems that have been studied to date use template molecules composed of nucleic acids (6,7,10), peptides (11)(12)(13)(14), or small organic compounds (15)(16)(17). The nucleic acid-based systems are the most straightforward and rely on simple Watson-Crick pairing interactions between a short oligonucleotide template and two complementary oligonucleotide substrates (6,7,10).…”

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confidence: 99%

“…Peptide-based self-replicating systems are similar, except that the components are oligopeptides that have the capacity to form ␣-helices. The template is the hydrophobic face of an ␣-helix that interacts with the corresponding face of two peptide substrates (11)(12)(13)(14). Unlike nucleic acid systems, where the templating interactions involve all of the nucleotide subunits, peptide replication systems involve only a few amino acid residues in the helix-helix interactions.…”

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confidence: 99%

A self-replicating ligase ribozyme

Paul

Joyce²

2002

Proc. Natl. Acad. Sci. U.S.A.

234

161

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A self-replicating molecule directs the covalent assembly of component molecules to form a product that is of identical composition to the parent. When the newly formed product also is able to direct the assembly of product molecules, the self-replicating system can be termed autocatalytic. A self-replicating system was developed based on a ribozyme that catalyzes the assembly of additional copies of itself through an RNA-catalyzed RNA ligation reaction. The R3C ligase ribozyme was redesigned so that it would ligate two substrates to generate an exact copy of itself, which then would behave in a similar manner. This self-replicating system depends on the catalytic nature of the RNA for the generation of copies. A linear dependence was observed between the initial rate of formation of new copies and the starting concentration of ribozyme, consistent with exponential growth. The autocatalytic rate constant was 0.011 min ؊1 , whereas the initial rate of reaction in the absence of pre-existing ribozyme was only 3.3 ؋ 10 ؊11 M⅐min ؊1 . Exponential growth was limited, however, because newly formed ribozyme molecules had greater difficulty forming a productive complex with the two substrates. Further optimization of the system may lead to the sustained exponential growth of ribozymes that undergo self-replication. In living systems, replicative processes transfer genetic information from template nucleic acid molecules to newly synthesized, complementary products. Several nonenzymatic templatedependent ligation systems have been devised to study the role of a template in binding and positioning complementary substrates for covalent bond formation (1-5). These have included simple self-replicating systems of the form A ϩ B 3 T, where A and B are substrates that bind to a complementary template, T, and become joined to form a product molecule that is identical to the template (6-9). The unique aspect of self-replicating systems is that the reaction product has the potential to direct additional reactions. The system is termed autocatalytic when the product is an efficient template, and each covalent bond that is formed generates additional template molecules that can direct further joining reactions. The realization of autocatalytic behavior in a self-replicating system implies that sustained exponential growth may be possible.The self-replicating systems that have been studied to date use template molecules composed of nucleic acids (6, 7, 10), peptides (11-14), or small organic compounds (15-17). The nucleic acid-based systems are the most straightforward and rely on simple Watson-Crick pairing interactions between a short oligonucleotide template and two complementary oligonucleotide substrates (6, 7, 10). The substrates are bound at adjacent positions along the template and are joined through a reaction involving chemical groups at their opposed ends. Peptide-based self-replicating systems are similar, except that the components are oligopeptides that have the capacity to form ␣-helices. The template is the hydrophob...

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A pH-Modulated, Self-Replicating Peptide

Cited by 119 publications

References 30 publications

Asymmetric Autocatalysis and Its Implications for the Origin of Homochirality

Asymmetric Autocatalysis and Its Implications for the Origin of Homochirality

RETRACTED: Replication of an RNA ligase ribozyme under alternating temperature condition

A self-replicating ligase ribozyme

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