A Phage Display Screening Derived Peptide with Affinity for the Adeninyl Moiety

Elmlund, Louise; Söderberg, Pernilla; Suriyanarayanan, Subramanian; Nicholls, Ian A.

doi:10.3390/bios4020137

Cited by 11 publications

(6 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Here, we notice that this peak increases in intensity and undergoes a red shift (from 1575 to 1570 cm

) when DNA is absorbed on HNs. A red shift of this mode was also observed by Elmlund et al [ 41 ] for Adenine absorbed onto quartz surface. Therefore, the shift of

asymmetric stretching from 1242 cm

(observed in dehydrated DNA with both far-field and nano-FTIR spectroscopy) to 1253 cm

(in DNA–HNs), together with the strong intensity reduction of the 1698 cm

peak (associated to thymine C2=O2 moiety) and the intensity increase of the peak at 1570 cm

(linked to Adenine N-H) in DNA–HNs spectra, point towards the occurrence of both base-specific and backbone structural variations of DNA when adsorbed on HNs.…”

Section: Resultssupporting

confidence: 80%

Infrared Nanospectroscopy Reveals DNA Structural Modifications upon Immobilization onto Clay Nanotubes

et al. 2021

View full text Add to dashboard Cite

The growing demand for innovative means in biomedical, therapeutic and diagnostic sciences has led to the development of nanomedicine. In this context, naturally occurring tubular nanostructures composed of rolled sheets of alumino-silicates, known as halloysite nanotubes, have found wide application. Halloysite nanotubes indeed have surface properties that favor the selective loading of biomolecules. Here, we present the first, to our knowledge, structural study of DNA-decorated halloysite nanotubes, carried out with nanometric spatially-resolved infrared spectroscopy. Single nanotube absorption measurements indicate a partial covering of halloysite by DNA molecules, which show significant structural modifications taking place upon loading. The present study highlights the constraints for the use of nanostructured clays as DNA carriers and demonstrates the power of super-resolved infrared spectroscopy as an effective and versatile tool for the evaluation of immobilization processes in the context of drug delivery and gene transfer.

show abstract

“…Here, we notice that this peak increases in intensity and undergoes a red shift (from 1575 to 1570 cm

) when DNA is absorbed on HNs. A red shift of this mode was also observed by Elmlund et al [ 41 ] for Adenine absorbed onto quartz surface. Therefore, the shift of

asymmetric stretching from 1242 cm

(observed in dehydrated DNA with both far-field and nano-FTIR spectroscopy) to 1253 cm

(in DNA–HNs), together with the strong intensity reduction of the 1698 cm

peak (associated to thymine C2=O2 moiety) and the intensity increase of the peak at 1570 cm

(linked to Adenine N-H) in DNA–HNs spectra, point towards the occurrence of both base-specific and backbone structural variations of DNA when adsorbed on HNs.…”

Section: Resultssupporting

confidence: 80%

Infrared Nanospectroscopy Reveals DNA Structural Modifications upon Immobilization onto Clay Nanotubes

et al. 2021

View full text Add to dashboard Cite

show abstract

“…A comparison of this study with previously reported works is presented in Table S1 (Supporting Information). Furthermore, the values of the association constant ( K a ) and disassociation constant ( K d ) for the BSA/Ab/PANnf/ITO immunoelectrode were estimated with the Hanes–Wolf plot [graph between the antigen concentration and the antigen concentration divided by the current, Figure A(c)]. The inverse value of the slope observed by the linear fitting of the Hanes–Wolf plot gave a value of K a of 45.2 ng/mL, whereas the product of the K a and intercept gave a value of K d of 8 ng/mL.…”

Section: Resultsmentioning

confidence: 99%

Functionalized polyacrylonitrile‐nanofiber based immunosensor for Vibrio cholerae detection

Gupta

Dhakate

et al. 2016

J of Applied Polymer Sci

View full text Add to dashboard Cite

Polyacrylonitrile nanofibers (PANnf's) were electrospun directly onto an indium tin oxide (ITO)‐coated glass substrate. The PANnf/ITO electrode was partially hydrolyzed with an NaOH aqueous solution at ambient temperature to convert the nitrile groups of the PANnf's into carboxyl groups was confirmed by Fourier transform infrared spectroscopy. Furthermore, 1‐ethyl‐3‐(3‐dimethylaminopropyl) carbodiimide–N‐hydroxy succinimide chemistry was used to activate the COOH groups of PANnf's for the covalent co‐immobilization of monoclonal antibodies against Vibrio cholerae and bovine serum albumin for V. cholerae toxin detection. Structural, functional, and electrochemical studies of the PANnf/ITO electrode and BSA/Ab/PANnf/ITO immunoelectrode were performed, and found a uniform distribution of nanofibers with diameter of 325 ± 7.7% nm. The electrochemical response studies showed an improved sensing performance of the immunoelectrode with a detection of 6.25–500 ng/mL, a low limit of detection of 0.22 ng/mL, a sensitivity of 90 nA ng−1 mL cm−2, an association constant of 45.2 ng/mL, and a dissociation constant of 8 ng/mL. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 44170.

show abstract

“…The value of low detection limit calculated using formula 3σ b /m criteria, where m is the slope of the calibration graph and σ b is the standard deviation of the blank signal. Furthermore, the value of association (K a ) for the BSA/Ab/ZnO/ITO immunoelectrode is estimated using the Hanes-Wolf plot 37,41 (graph between [antigen concentrations] and [antigen concentration/current]) as shown in Fig. 5C.…”

Section: Electrochemical Studies-optimization Of Electrochemicalmentioning

confidence: 99%

One-Step Electrodeposited Porous ZnO Thin Film Based Immunosensor for Detection ofVibrio choleraeToxin

Gupta

Khan

Solanki

2016

J. Electrochem. Soc.

View full text Add to dashboard Cite

Here, we have reported one step fabrication of a porous thin film of the zinc oxide nanoparticles (ZnO NPs) directly onto the indium tin oxide (ITO) glass substrate by cathodic electrodeposition from an aqueous solution containing zinc nitrate. The morphological studies of a thin film of ZnO NPs suggest that the NPs formed a porous structure with irregular pore size distribution over the surface of ITO glass substrate. The average pore size and wall thickness of pores obtained as 620 nm and 50-120 nm, respectively. Transmission electron microscopic investigations reveal the growth of spherical ZnO NPs of size ranging from 20-30 nm. The surface charge on the electrodeposited ZnO NPs has shown a positive value of 18 mV as confirmed by zeta potential. This electrodeposited porous thin film of ZnO NPs has been employed for immobilization of monoclonal antibodies (Ab) specific to Vibrio cholerae for detection of Vibrio cholerae toxin B. Electrochemical studies reveal that these ZnO/ITO electrodes provide favorable conditions for immobilization of Ab with better orientation, resulting in enhanced electron transfer at the interface. This immunoelectrode exhibits improved biosensing characteristics as detection range varies from 12.5-500 ng mL −1 , the sensitivity of 71 nA ng −1 mL cm −2 , the low detection limit of 0.16 ng mL −1 and an association constant of 14.12 ng mL −1 . Significantly, the electrodeposited ZnO thin films are uniform, cost-effective, reproducible and stable. This can be processed for commercialization.

show abstract

A Phage Display Screening Derived Peptide with Affinity for the Adeninyl Moiety

Cited by 11 publications

References 28 publications

Infrared Nanospectroscopy Reveals DNA Structural Modifications upon Immobilization onto Clay Nanotubes

Infrared Nanospectroscopy Reveals DNA Structural Modifications upon Immobilization onto Clay Nanotubes

Functionalized polyacrylonitrile‐nanofiber based immunosensor for Vibrio cholerae detection

One-Step Electrodeposited Porous ZnO Thin Film Based Immunosensor for Detection ofVibrio choleraeToxin

Contact Info

Product

Resources

About