A Phenotypic Analysis of Regulatory T Cells and Uterine <scp>NK</scp> Cells from First Trimester Pregnancies and Associations with <scp>HLA</scp>‐G

Djurisic, Snezana; Skibsted, Lillian; Hviid, Thomas Vauvert F.

doi:10.1111/aji.12421

Cited by 30 publications

(20 citation statements)

References 58 publications

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“…Interestingly the CD4 C ILT2 C , CD8 C ILT2 C and CD8 low T cells found in tumor areas are known in the literature as suppressor cells. 23,24,25 One can hypothesize that in such tumor regions where these suppressor cells predominate, the anti-tumor response will be particularly inhibited. Thus, our results show that each tumor area will develop specific escape mechanisms from immunological attack.…”

Section: Discussionmentioning

confidence: 99%

Intratumor heterogeneity of immune checkpoints in primary renal cell cancer: Focus on HLA-G/ILT2/ILT4

et al. 2017

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The establishment and maintenance of anti-tumor immune responses are the objectives of cancer immunotherapy. Despite recent promising advances, the effectiveness of these approaches has been limited by the multiple immunosuppressive mechanisms developed by tumors (checkpoint). The aim of the present study was to demonstrate intratumor heterogeneity at the levels of immune escape strategies and tumor-host relationships. We focused on well-known checkpoints such as PD1/PDL1 and on a new checkpoint involving HLA-G and its receptors ILT2/ILT4. A prospective study was performed on 19 renal-cell carcinoma patients that were included during hospitalization for surgical tumor resection. Different areas of the tumor were collected for each patient and subjected to both immunohistochemical and flow cytometry analysis. Immune cells from peripheral blood were concomitantly analyzed for each patient. Our results show the heterogeneous expression of PD1/PDL1 and HLA-G/ILT in the various areas of the same tumor. Intratumor heterogeneity was found both at tumor cell and infiltrating immune cell levels. From a clinical point of view, this work highlights the functional redundancies of checkpoints and the need to adapt personalized poly-immunotherapy.

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Section: Discussionmentioning

confidence: 99%

Intratumor heterogeneity of immune checkpoints in primary renal cell cancer: Focus on HLA-G/ILT2/ILT4

et al. 2017

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“…Finally, a correlation was found between uterine sHLA-G and the fraction of KIR2DL4+ uterine NK cells. The authors hypothesize that the phenotype of uterine NK cells may be influenced by HLA-G on trophoblast cells and by sHLA-G in the uterus [ 47 ].…”

Section: Hla-g and Pregnancymentioning

confidence: 99%

Recent Advances in Our Understanding of HLA-G Biology: Lessons from a Wide Spectrum of Human Diseases

Morandi

Rizzo

Fainardi

et al. 2016

Journal of Immunology Research

110

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HLA-G is a HLA-class Ib molecule with potent immunomodulatory activities, which is expressed in physiological conditions, where modulation of the immune response is required to avoid allograft recognition (i.e., maternal-fetal interface or transplanted patients). However, HLA-G can be expressed de novo at high levels in several pathological conditions, including solid and hematological tumors and during microbial or viral infections, leading to the impairment of the immune response against tumor cells or pathogens, respectively. On the other hand, the loss of HLA-G mediated control of the immune responses may lead to the onset of autoimmune/inflammatory diseases, caused by an uncontrolled activation of the immune effector cells. Here, we have reviewed novel findings on HLA-G functions in different physiological and pathological settings, which have been published in the last two years. These studies further confirmed the important role of this molecule in the modulation of the immune system.

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“…Interestingly, there were no differences in CD8 + T cells among the sample origins or the obstetrical study groups. The RPB taken immediately after delivery may reflect maternal-fetal interface blood with the potential mixture of maternal blood [39]. We named it as "retro placental blood" in this study.…”

Section: Discussionmentioning

confidence: 99%

Maternal and Fetal CD4+CD25+CD127low/- Regulatory T Cells in Pregnancies with Gestational Diabetes, Preeclampsia, and Premature Rupture of Membranes

Zhao

Zhang

Sun

et al. 2020

Preprint

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Background Regulatory T cells (Tregs) play a crucial role in maternal-fetal tolerance, but little is known about the characteristic of Tregs in peripheral blood (PB), maternal-fetal interface and cord blood (CB) in normal and complicated pregnancies with preeclampsia (PE), gestational diabetes mellitus (GDM) and premature rupture of membranes (PROM). Methods PB, retro-placental blood (RPB), and CB were collected immediately after delivery in women with normal full-term pregnancy (NP), PE, GDM, and PROM. The proportion of CD4 + CD25 + CD127 low/- T cells (Tregs) and the expression of PD-1, GITR, HLA-G and CTLA-4 on T cell subsets were investigated by flow cytometric analysis. The data were analyzed based on sample origins (PB, RPB, and CB) and the obstetrical study groups (NP, PE, GDM, and PROM). Results The proportions of Tregs in PB, RPB, and CB from NP were significantly higher than the other obstetrical groups (P < 0.01, respectively). However no significant differences were present among the PE, GDM and PROM groups (P = NS). The proportion of PD-1 + and GITR + Tregs in RPB and PB as well as PD-1 + Tregs in CB from NP were significantly higher than those of the same origin in each obstetrical study group (P < 0.01, respectively). There were no differences in HLA-G + and CTLA-4 + Tregs between different origins in each obstetrical study group. In NP, the proportion of PD-1 + Tregs was significantly decreased in CB as compared to those of PB and RPB (P < 0.05, respectively). Among the four groups, the proportion of GITR + Tregs were significantly higher in PB as compared to those of CB and RPB (P < 0.01, respectively). The proportion of HLA-G + Tregs in PB was significantly lower than that of CB and RPB (P < 0.01, respectively). The proportion of CTLA-4 + Tregs had no significant differences (P = NS). Conclusions In conclusion, the proportion and characteristics of Tregs vary in the maternal, fetal and maternal-fetal junction at the time of delivery. The different feature and density of Tregs in maternal and fetal tissue may suggest the multiple and complicated roles of Tregs during pregnancy.

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A Phenotypic Analysis of Regulatory T Cells and Uterine NK Cells from First Trimester Pregnancies and Associations with HLA‐G

Abstract: The NK subset in the placental bed displays a unique phenotype that may be influenced by mHLA-G on trophoblast cells and locally accumulated sHLA-G in the uterus.

Cited by 30 publications

References 58 publications

Intratumor heterogeneity of immune checkpoints in primary renal cell cancer: Focus on HLA-G/ILT2/ILT4

Intratumor heterogeneity of immune checkpoints in primary renal cell cancer: Focus on HLA-G/ILT2/ILT4

Recent Advances in Our Understanding of HLA-G Biology: Lessons from a Wide Spectrum of Human Diseases

Maternal and Fetal CD4+CD25+CD127low/- Regulatory T Cells in Pregnancies with Gestational Diabetes, Preeclampsia, and Premature Rupture of Membranes

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