A photo-regulated aptamer sensor for spatiotemporally controlled monitoring of ATP in the mitochondria of living cells

Hong, Shanni; Zhang, Xiaoting; Lake, Ryan J.; Pawel, Gregory T.; Guo, Zijian; Pei, Renjun; Lu, Yi

doi:10.1039/c9sc04773e

Cited by 69 publications

(59 citation statements)

References 58 publications

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“…The well-matched intensity peaks confirm the excellent mitochondria-targeting ability of SiR 8 in live cells. [22] In addition, CLSM images of live HepG2 cells stained with 10 μg/mL SiR 8 at different sections through the zaxis were performed in ESI, Figure S10. On the contrary, the fluorescence from SiR 8 didn't overlap with that from Lyso Tracker Green ( Figure S11) and Hoechst 33342 ( Figure S12).…”

Section: Resultsmentioning

confidence: 99%

“…An intensity profile of the mitochondria marked with a line in Figure c was used to investigate the degree of the colocation as demon‐strated in Figure d. The well‐matched intensity peaks confirm the excellent mitochondria‐targeting ability of SiR 8 in live cells . In addition, CLSM images of live HepG2 cells stained with 10 μg/mL SiR 8 at different sections through the zaxis were performed in ESI, Figure S10.…”

Section: Resultsmentioning

confidence: 99%

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A General Strategy for the Construction of NIR‐emitting Si‐rhodamines and Their Application for Mitochondrial Temperature Visualization

Tang

Gao

et al. 2020

Chemistry An Asian Journal

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Si‐rhodamine (SiR) is an ideal fluorophore because it possesses bright emission in the NIR region and can be implemented flexibly in living cells. Currently, several promising approaches for synthesizing SiR are being developed. However, challenges remain in the construction of SiR containing functional groups for bioimaging application. Herein, we introduce a general and simple approach by a condensation reaction of diarylsilylether and arylaldehyde in o‐dichlorobenzene to synthesize a series of SiRs bearing various functional substituents. These SiRs have moderate to high quantum efficiency, tolerance to photobleaching, and high water solubility as well as NIR emitting, and their NIR fluorescence properties can be controlled through the photoinduced electron transfer (PET) mechanism. Fluorescence OFF‐ON switching effect is observed for SiR 9 in the presence of acid, which is rationalized by DFT/TDDFT calculations. Moreover, reversible stimuli response toward temperature is achieved. Since positive charge enables mitochondrial targeting ability and chloromethyl unit can covalently immobilize the dyes onto the mitochondrial via click reaction between the benzyl choride and protein sulfhydryls, SiR 8 is identified as a valuable fluorescent marker to visualize the morphology and monitor the temperature change of mitochondria with high photostability.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

A General Strategy for the Construction of NIR‐emitting Si‐rhodamines and Their Application for Mitochondrial Temperature Visualization

Tang

Gao

et al. 2020

Chemistry An Asian Journal

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“…Fluorescence was only observed after light activation when the aptamer was freed to bind ATP and adopt a new conformation that displaced the quencher. The sensor successfully detected ATP concentration fluctuations caused by Ca 2+ or oligomycin treatment [ 42 ].…”

Section: Methods For Blocking the Function Of Oligosmentioning

confidence: 99%

Conditionally Activated (“Caged”) Oligonucleotides

Yang

Dmochowski

2021

Molecules

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Conditionally activated (“caged”) oligonucleotides provide useful spatiotemporal control for studying dynamic biological processes, e.g., regulating in vivo gene expression or probing specific oligonucleotide targets. This review summarizes recent advances in caging strategies, which involve different stimuli in the activation step. Oligo cyclization is a particularly attractive caging strategy, which simplifies the probe design and affords oligo stabilization. Our laboratory developed an efficient synthesis for circular caged oligos, and a circular caged antisense DNA oligo was successfully applied in gene regulation. A second technology is Transcriptome In Vivo Analysis (TIVA), where caged oligos enable mRNA isolation from single cells in living tissue. We highlight our development of TIVA probes with improved caging stability. Finally, we illustrate the first protease-activated oligo probe, which was designed for caspase-3. This expands the toolkit for investigating the transcriptome under a specific physiologic condition (e.g., apoptosis), particularly in specimens where light activation is impractical.

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“…For specific targeting of fluorescent probes, polymer-based delivery systems have been also used. Hong et al developed a photocaged aptamer-based ATP sensor ( 28 ) that is delivered to the mitochondria using liposome-based polymeric transporters ( Figure 6 ) [ 65 ]. Because of their high selectivity and sensitivity achieved by performing multiple selection cycles, aptamer-based probes and reagents were in demand with respect to various applications in recent years [ 66 , 67 , 68 ].…”

Section: Design Strategies and Recent Examplesmentioning

confidence: 99%

Recent Advances in Organelle-Targeted Fluorescent Probes

et al. 2021

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Recent advances in fluorescence imaging techniques and super-resolution microscopy have extended the applications of fluorescent probes in studying various cellular processes at the molecular level. Specifically, organelle-targeted probes have been commonly used to detect cellular metabolites and transient chemical messengers with high precision and have become invaluable tools to study biochemical pathways. Moreover, several recent studies reported various labeling strategies and novel chemical scaffolds to enhance target specificity and responsiveness. In this review, we will survey the most recent reports of organelle-targeted fluorescent probes and assess their general strategies and structural features on the basis of their target organelles. We will discuss the advantages of the currently used probes and the potential challenges in their application as well as future directions.

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A photo-regulated aptamer sensor for spatiotemporally controlled monitoring of ATP in the mitochondria of living cells

Abstract: A photo-regulated ATP sensor coupled with cationic DQAsomes is developed for spatiotemporally controlled imaging of ATP in the mitochondria of living cells.

Cited by 69 publications

References 58 publications

A General Strategy for the Construction of NIR‐emitting Si‐rhodamines and Their Application for Mitochondrial Temperature Visualization

A General Strategy for the Construction of NIR‐emitting Si‐rhodamines and Their Application for Mitochondrial Temperature Visualization

Conditionally Activated (“Caged”) Oligonucleotides

Recent Advances in Organelle-Targeted Fluorescent Probes

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