A photoelectrochemical immunosensor based on magnetic all-solid-state Z-scheme heterojunction for SARS-CoV-2 nucleocapsid protein detection

Guo, Aijiao; Pei, Fubin; Feng, Shasha; Hu, Wei; Zhang, Pengjie; Xia, Mingzhu; Mu, Xiyan; Tong, Zhaoyang; Wang, Fengyun; Liu, Bing

doi:10.1016/j.snb.2022.132800

Cited by 15 publications

(4 citation statements)

References 34 publications

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“…Then, the authors developed a photoelectrochemical immunosensor to detect NP with a wide linear range of 10 pg mL −1 to 100 ng mL −1 and low detection limit down to 2.9 pg mL −1 . 342 The Si-FITC NPs prepared by Mao et al not only solved the problem of reduced quantum yield during the coupling reaction but also achieved the sensitive detection of NP (LOD of 3 pg mL −1 ) ( Fig. 12C ).…”

Section: Sars-cov-2 Antigen Detectionmentioning

confidence: 99%

Diagnostics and analysis of SARS-CoV-2: current status, recent advances, challenges and perspectives

et al. 2023

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Section: Sars-cov-2 Antigen Detectionmentioning

confidence: 99%

Diagnostics and analysis of SARS-CoV-2: current status, recent advances, challenges and perspectives

et al. 2023

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“…Table 1 [46][47][48][49][50][51][52][53][54][55][56][57][58] shows different techniques and analytical parameters reported in the literature for the detection of SARS-CoV-2. As can be seen, the proposed immunosensors reported in this manuscript presents a sensitivity comparable to or better than the analytical sensitivity reported in some works.…”

Section: Electrochemical Characterization Of the Pec Sensormentioning

confidence: 99%

“…Colorimetric S protein 10-100 ng mL À 1 11 ng mL À 1 -- [51] Field-effect transistor S protein 1 fg mL À 1 -10 pg mL À 1 1 fg mL Photoelectrochemical N protein 0.001-50 ng mL À 1 0.38 pg mL À 1 0.0 100 W LED [55] Photoelectrochemical N protein 10 pg mL À 1 -100 ng mL À 1 2.9 pg mL À 1 0.0 300 W Xe [56] Photoelectrochemical S protein 0.1-1000 ng mL À 1 0.53 ng mL À 1 0.1 300 W Xe [57] Photoelectrochemical S protein 0.5-32 nM 0.12 nM 0.05 150 W Xe [58] S protein 0.1 pg mL À 1 -1000 ng mL À 1 0.1 pg mL À 1 0.0 36 W LED T.w Photoelectrochemical N protein 0.01 pg mL À 1 -1000 ng mL À 1 0.01 pg mL À 1 *PLpro-Papain-like cysteine protease. T.w.…”

Section: Light Source Referencementioning

confidence: 99%

Photoelectrochemical biosensing platform for the SARS‐CoV‐2 spike and nucleocapsid proteins

et al. 2023

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The COVID‐19 pandemic is still a continuing worldwide challenge for public health systems. Early and ultrasensitive identification of the infection is essential for preventing the spread of COVID‐19 by pre‐symptomatic or asymptomatic individuals, particularly in the community and in‐home settings. This work presents a versatile photoelectrochemical (PEC) immunosensor for SARS‐CoV‐2 detection based on a composite material formed by bismuth vanadate (BiVO4) and strontium titanate (SrTiO3). The PEC platform was denoted as BiVO4/SrTiO3/FTO, and it can be tuned for the detection of either Spike (S) or Nucleocapsid (N) protein by simply altering the antibody immobilized on the platform's surface. Chemical, morphological, and electrochemical characterizations were performed by X‐Ray Diffraction, Scanning Electron microscopy, Energy‐dispersive X‐ray spectroscopy, Electrochemical Impedance Spectroscopy, and Amperometry. With a simple sensing architecture of the PEC platform, it was possible to achieve a linear response range of 0.1 pg mL−1 to 1000 ng mL−1 for S protein and 0.01 pg mL−1 to 1000 ng mL−1 for N protein. The PEC immunosensors presented recovery values for the two SARS‐CoV‐2 proteins in artificial saliva samples between 97 % and 107.20 % suggesting a good accuracy for the proposed immunosensors.

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“…Additionally, the nucleocapsid (N) protein is one of the most abundant SARS-CoV-2 proteins, with vigorous immunogenicity and is highly expressed during infection [3]. Furthermore, SARS-CoV-2 N protein is an extremely important candidate protein for the development of various diagnostic methods for novel coronavirus diseases [4]. At present, there are some reliable analytical methods for SARS-CoV-2 N protein such as chemiluminescence immunoassays [5], enzyme-linked immunosorbent assays (ELISAs) [6], lateral flow immunoassays (LFAs) [7] and biosensing immunoassays [8].…”

Section: Introductionmentioning

confidence: 99%

A Novel SPR Immunosensor Based on Dual Signal Amplification Strategy for Detection of SARS-CoV-2 Nucleocapsid Protein

Fan,

Du,

Pei

et al. 2023

Biosensors

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Since the global outbreak of coronavirus disease 2019 (COVID-19), it has spread rapidly around the world. The nucleocapsid (N) protein is one of the most abundant SARS-CoV-2 proteins. Therefore, a sensitive and effective detection method for SARS-CoV-2 N protein is the focus of research. Here, we developed a surface plasmon resonance (SPR) biosensor based on the dual signal-amplification strategy of Au@Ag@Au nanoparticles (NPs) and graphene oxide (GO). Additionally, a sandwich immunoassay was utilized to sensitively and efficiently detect SARS-CoV-2 N protein. On the one hand, Au@Ag@Au NPs have a high refractive index and the capability to electromagnetically couple with the plasma waves propagating on the surface of gold film, which are harnessed for amplifying the SPR response signal. On the other hand, GO, which has the large specific surface area and the abundant oxygen-containing functional groups, could provide unique light absorption bands that can enhance plasmonic coupling to further amplify the SPR response signal. The proposed biosensor could efficiently detect SARS-CoV-2 N protein for 15 min and the detection limit for SARS-CoV-2 N protein was 0.083 ng/mL, with a linear range of 0.1 ng/mL~1000 ng/mL. This novel method can meet the analytical requirements of artificial saliva simulated samples, and the developed biosensor had a good anti-interference capability.

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A photoelectrochemical immunosensor based on magnetic all-solid-state Z-scheme heterojunction for SARS-CoV-2 nucleocapsid protein detection

Cited by 15 publications

References 34 publications

Diagnostics and analysis of SARS-CoV-2: current status, recent advances, challenges and perspectives

Diagnostics and analysis of SARS-CoV-2: current status, recent advances, challenges and perspectives

Photoelectrochemical biosensing platform for the SARS‐CoV‐2 spike and nucleocapsid proteins

A Novel SPR Immunosensor Based on Dual Signal Amplification Strategy for Detection of SARS-CoV-2 Nucleocapsid Protein

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