A Photonic crystal slab-based Ultrasonic HydrophoneE. Y. Zhu

Charles, Maria C.; Rewcastle, Cory; Gad, Raanan; Qian, Li; Levi, Ofer

doi:10.1109/pn50013.2020.9167023

Cited by 10 publications

(17 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…1,2 Unfortunately, low-abundance proteins are often not detectable in mass spectrometry (MS)-based proteomic analysis due to insufficient detection sensitivity and limited MS sequencing speed. While one-dimensional liquid chromatography (LC)-MS/MS analyses exhibit a high degree of reproducibility, short analysis times, and low sample requirements, [3][4][5] the proteome coverage is usually below 3000-4000 proteins due to limited peak capacities even with increased column lengths and gradient times. [6][7][8][9] Using the latestgeneration MS instrumentation, such as Bruker timsTOF Pro and Thermo-Fisher FAIMS-Lumos, proteome coverage could increase to 6000-7000 proteins 10,11 by injecting microgram-scale protein digest.…”

Section: Graphical Abstractmentioning

confidence: 99%

Automated Nanoflow Two-Dimensional Reversed-Phase Liquid Chromatography System Enables In-Depth Proteome and Phosphoproteome Profiling of Nanoscale Samples

et al. 2019

View full text Add to dashboard Cite

Two-dimensional reversed-phase capillary liquid chromatography (2D RPLC) separations have enabled comprehensive proteome profiling of biological systems. However, milligram sample quantities of proteins are typically required due to significant losses during offline fractionation. Such a large sample requirement generally precludes the application samples in the nanogram to low-microgram range. To achieve in-depth proteomic analysis of such small-sized samples, we have developed the nanoFAC (nanoflow Fractionation and Automated Concatenation) 2D RPLC platform, in which the first dimension high-pH fractionation was performed on a 75-μm i.d. capillary column at a 300 nL/min flow rate with automated fraction concatenation, instead of on a typically used 2.1 mm column at a 200 μL/min flow rate with manual concatenation. Each fraction was then fully transferred to the second-dimension low-pH nanoLC separation using an autosampler equipped with a custom-machined syringe. We have found that using a polypropylene 96-well plate as collection device as well as the addition of n-Dodecyl β-D-maltoside (0.01%) in the collection buffer can significantly improve sample recovery. We have demonstrated the nanoFAC 2D RPLC platform can achieve confident identifications of ~49,000–94,000 unique peptides, corresponding to ~6,700–8,300 protein groups using only 100–1000 ng of HeLa tryptic digest (equivalent to ~500–5,000 cells). Furthermore, by integrating with phosphopeptide enrichment, the nanoFAC 2D RPLC platform can identify ~20,000 phosphopeptides from 100 μg of MCF-7 cell lysate.

show abstract

Section: Graphical Abstractmentioning

confidence: 99%

Automated Nanoflow Two-Dimensional Reversed-Phase Liquid Chromatography System Enables In-Depth Proteome and Phosphoproteome Profiling of Nanoscale Samples

et al. 2019

View full text Add to dashboard Cite

show abstract

“…17,[19][20][21] We recently developed a microfluidic nanodroplet sample preparation approach, termed nanoPOTS (Nanodroplet Processing in One-pot for Trace Samples), that enables single cell proteomic sample processing and analysis using ultrasensitive nanoLC-MS/MS. [22][23][24] NanoPOTS reduces the sample processing volume to less than 200 nanoliters (nL) and the total exposure surfaces to ~1 mm 2 , thus significantly limiting surface losses during sample processing, which is critical for single cell proteomics. 22,25 Using the nanoPOTS platform, an average of 670 protein groups were confidently identified from single HeLa cells 23 and 160 proteins from a single spiked circulating tumor cells isolated from whole blood 26 .…”

Section: Introductionmentioning

confidence: 99%

“…[22][23][24] NanoPOTS reduces the sample processing volume to less than 200 nanoliters (nL) and the total exposure surfaces to ~1 mm 2 , thus significantly limiting surface losses during sample processing, which is critical for single cell proteomics. 22,25 Using the nanoPOTS platform, an average of 670 protein groups were confidently identified from single HeLa cells 23 and 160 proteins from a single spiked circulating tumor cells isolated from whole blood 26 . While the current nanoPOTS workflow yielded good single cell proteome coverage, the analytical throughput was relatively low at ~8 single cells per day.…”

Section: Introductionmentioning

confidence: 99%

High-Throughput Single Cell Proteomics Enabled by Multiplex Isobaric Labeling in a Nanodroplet Sample Preparation Platform

et al. 2019

View full text Add to dashboard Cite

Effective extension of mass spectrometry-based proteomics to single cells remains challenging. Herein we combined microfluidic nanodroplet technology with tandem mass tag (TMT) isobaric labeling to significantly improve analysis throughput and proteome coverage for single mammalian cells. Isobaric labeling facilitated multiplex analysis of single cell-sized protein quantities to a depth of ~1,600 proteins with median CV of 10.9% and correlation coefficient of 0.98. To demonstrate in-depth high throughput single cell analysis, the platform was applied to measure protein expression in 72 single cells from three murine cell populations (epithelial, immune, and endothelial cells) in <2 days instrument time with over 2,300 proteins identified. Principal component analysis grouped the single cells into three distinct populations based on protein expression with each population characterized by well-known cell-type specific markers. Our platform enables high throughput and unbiased characterization of single cell heterogeneity at the proteome level.

show abstract

“…Anions from lithium salts bind to the metal ions in MOFs and form ionic channels for lithium ions, resulting in ionic conductivities as high as 10 −4 S cm −1 . [9][10][11] While these values are only roughly equal to those of commercial separators, 12 the higher lithium transference numbers observed in these pseudo-solid-state electrolytes due to the reduced anionic contribution portend to increased rate performance in electrochemical energy storage devices. 10,11 The MOF-based pseudo-solid-state electrolytes are extremely promising for microscale electrochemical energy storage devices (e.g., thin-film batteries).…”

Section: Graphical Abstract Introductionmentioning

confidence: 99%

Conformal Ultrathin Film Metal–Organic Framework Analogues: Characterization of Growth, Porosity, and Electronic Transport

et al. 2019

View full text Add to dashboard Cite

Thin-film formation and transport properties of two copper-paddlewheel metal-organic framework (MOF) -based systems (MOF-14 and MOF-399) are investigated for their potential integration into electrochemical device architectures. Thin-film analogs of these two systems are fabricated by the sequential, alternating, solution-phase deposition of the inorganic and organic ligand precursors that result in conformal films via van der Merwe-like growth. Atomic force microscopy reveals smooth film morphologies with surface roughnesses determined by the underlying substrates and linear film growth of 1.4 and 2.2 nm per layer for the MOF-14 and MOF-399 systems, respectively. Electrochemical impedance spectroscopy is used to evaluate the electronic transport properties of the thin films, finding that the MOF-14 analog films demonstrate low electronic conductivity, while MOF-399 analog films are electronically insulating. The intrinsic porosities of these ultrathin MOF analog films are confirmed by cyclic voltammetry redox probe characterization using ferrocene. Larger peak currents are observed for MOF-399 analog films compared to MOF-14 analog films, which is consistent with the larger pores of MOF-399. The layer-by-layer deposition of these systems provides a promising route to incorporate MOFs as thin films with nanoscale thickness control and low surface roughness for electrochemical devices.

show abstract

A Photonic crystal slab-based Ultrasonic HydrophoneE. Y. Zhu

Cited by 10 publications

References 3 publications

Automated Nanoflow Two-Dimensional Reversed-Phase Liquid Chromatography System Enables In-Depth Proteome and Phosphoproteome Profiling of Nanoscale Samples

Automated Nanoflow Two-Dimensional Reversed-Phase Liquid Chromatography System Enables In-Depth Proteome and Phosphoproteome Profiling of Nanoscale Samples

High-Throughput Single Cell Proteomics Enabled by Multiplex Isobaric Labeling in a Nanodroplet Sample Preparation Platform

Conformal Ultrathin Film Metal–Organic Framework Analogues: Characterization of Growth, Porosity, and Electronic Transport

Contact Info

Product

Resources

About