A Phox2b+ Pontine Nucleus Essential for Ingestion

Abstract

The first phase of feeding consists in acquiring solid foods from the environment by biting, and their preparation for swallowing by chewing. These actions require the precise coordination of tens of orofacial muscles for the jaw and tongue. The siege for this motor patterning is known to be in the reticular formation, a complex and poorly mapped region of the hindbrain, but the neuron groups involved are still elusive. Here, we characterize a group of reticular interneurons located in the supratrigeminal area that express the homeodomain transcription factorPhox2b. This nucleus — Sup5Phox2b— is premotor to both jaw-closing and jaw-opener motoneurons and receives direct input from cranial sensory afferents, motor cortex and satiation related nuclei. Its activity differentially tracks lapping, biting and chewing movements, suggesting its involvement in the elaboration of distinct orofacial motor patterns in vivo. Acute global activation or inhibition of Sup5Phox2bby optogenetics both interrupt volitional feeding sequences. Thus, Sup5Phox2bis an obligatory subcortical node, topologically and genetically defined, in the neural circuits that control the oral phase of feeding.TeaserA genetically defined cluster of neurons in the hindbrain is an essential relay for biting and chewing food.