A Physiological and Molecular Analysis of the GenusNicotiana

Genetic and Evolutionaty Relationship Among Nicotiana Secies as Elucidated by AFLP

“…When compared with the low level of genetic variation observed among the cultivated N. tabacum accessions (Siva Raju et al, 2008), interspecific variations were significantly higher. The high degree of genetic polymorphism observed among the species in the present AFLP analysis was consistent with the results from the analysis of interspecific variations using RAPD (Bogani et al, 1997) and AFLP analysis in 8 Nicotiana species (Ren and Timko, 2001). In addition to detection of similar trends in interspecific variation, this approach gave very similar phylogenitic groupings which were consistent with the proposed clustering of species based on cytological and morphological characteristics (Narayan, 1987).…”

The Horizontal Transfer of Agrobacterium rhizogenes Genes and the Evolution of the Genus Nicotiana

“…1). More recently a further characterization of the genus has been carried out with physiological and molecular tools like the analysis of the morphophysiological in vitro behavior of explants from several species (Bogani et al, 1985(Bogani et al, , 1997 and studies of DNA multiplicity changes (Narayan, 1987) and of interspecific sequence variation through the use of a series of molecular markers like chloroplast RFLP, repeated sequences, and RAPD polymorphisms (Kung et al, 1982;Tassopulu and Kung, 1984;Koukalova et al, 1993;Komarinski et al, 1995;Hua et al, 1993;Borisjuk et al, 1994;Bogani et al, 1997;Yu and Lin, 1997).…”

Genetic Polymorphism between Tobacco Cultivar-groups Revealed by Amplified Fragment Length Polymorphism Analysis

“…(del Piano et al, 2004). Likewise, AFLP has been successfully used in genetic analysis of tobacco cultivars (Ren & Timko, 2001;Rossi et al, 2001;Zhang et al, 2006, Siva Raju et al, 2008and Zhang et al, 2008 and it is better than RAPD or ISSR for a narrow-genetic basis of cultivated crops because of its higher number of polymorphic loci per primer (Bogani et al, 1997). Furthermore, AFLP appears to be an appropriate method for processed tobacco leaves (Rossi et al, 2001).…”