A piezoelectric immunosensor for specific capture and enrichment of viable pathogens by quartz crystal microbalance sensor, followed by detection with antibody-functionalized gold nanoparticles

Guo, Xiao; Lin, Chih‐Sheng; Chen, Sz Hau; Ye, Rebecca; Wu, Vivian C.H.

doi:10.1016/j.bios.2012.05.024

Cited by 108 publications

(32 citation statements)

References 33 publications

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“…In surface acoustic wave sensors, the sensor's impedance changes when bacteria accumulate on its surface; this assists in detecting early bacterial attachment [18]. Another type of acoustic wave sensor uses a quartz crystal microbalance coupled with media enrichment for rapid detection of viable E. coli O157:H7 bacteria within 24 hours [19].…”

Section: Methods For Biofilm Detectionmentioning

confidence: 99%

Emerging frontiers in detection and control of bacterial biofilms

Tan

Chew

Tan

et al. 2014

Current Opinion in Biotechnology

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Section: Methods For Biofilm Detectionmentioning

confidence: 99%

Emerging frontiers in detection and control of bacterial biofilms

Tan

Chew

Tan

et al. 2014

Current Opinion in Biotechnology

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“…QCM sensors have been developed for the detection of whole bacterial cells, including Escherichia coli (56,57), Salmonella enterica serovar Typhimurium (58), Campylobacter jejuni (59) and Bacillus anthracis (60). The development of sandwich-type assays which employ nanoparticles for signal amplification has allowed for the detection of very few bacterial cells, down to 10 CFU/ml in some cases (58).…”

Section: Mechanical Biosensorsmentioning

confidence: 99%

Biosensors for Whole-Cell Bacterial Detection

et al. 2014

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SUMMARY Bacterial pathogens are important targets for detection and identification in medicine, food safety, public health, and security. Bacterial infection is a common cause of morbidity and mortality worldwide. In spite of the availability of antibiotics, these infections are often misdiagnosed or there is an unacceptable delay in diagnosis. Current methods of bacterial detection rely upon laboratory-based techniques such as cell culture, microscopic analysis, and biochemical assays. These procedures are time-consuming and costly and require specialist equipment and trained users. Portable stand-alone biosensors can facilitate rapid detection and diagnosis at the point of care. Biosensors will be particularly useful where a clear diagnosis informs treatment, in critical illness (e.g., meningitis) or to prevent further disease spread (e.g., in case of food-borne pathogens or sexually transmitted diseases). Detection of bacteria is also becoming increasingly important in antibioterrorism measures (e.g., anthrax detection). In this review, we discuss recent progress in the use of biosensors for the detection of whole bacterial cells for sensitive and earlier identification of bacteria without the need for sample processing. There is a particular focus on electrochemical biosensors, especially impedance-based systems, as these present key advantages in terms of ease of miniaturization, lack of reagents, sensitivity, and low cost.

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“…A novel QCM-based immunosensor with noble MNP enhancement was investigated for monitoring Escherichia coli O157 : H7 in food samples by employing antibody-NP conjugates as the detection complex. Conjugating antibodies with AuNPs as signal amplifier and employing brain heart infusion broth as growth medium to enrich the bacteria significantly improved the sensitivity of the sensor, and a detection limit of 0–10 log CFU/ml was obtained [210]. …”

Section: The Functions Of Mnps In Various Biosensor Typesmentioning

confidence: 99%

Noble metal nanoparticles in biosensors: recent studies and applications

Malekzad

Zangabad

Mirshekari

et al. 2017

Nanotechnology Reviews

305

119

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The aim of this review is to cover advances in noble metal nanoparticle (MNP)-based biosensors and to outline the principles and main functions of MNPs in different classes of biosensors according to the transduction methods employed. The important biorecognition elements are enzymes, antibodies, aptamers, DNA sequences, and whole cells. The main readouts are electrochemical (amperometric and voltametric), optical (surface plasmon resonance, colorimetric, chemiluminescence, photoelectrochemical, etc.) and piezoelectric. MNPs have received attention for applications in biosensing due to their fascinating properties. These properties include a large surface area that enhances biorecognizers and receptor immobilization, good ability for reaction catalysis and electron transfer, and good biocompatibility. MNPs can be used alone and in combination with other classes of nanostructures. MNP-based sensors can lead to significant signal amplification, higher sensitivity, and great improvements in the detection and quantification of biomolecules and different ions. Some recent examples of biomolecular sensors using MNPs are given, and the effects of structure, shape, and other physical properties of noble MNPs and nanohybrids in biosensor performance are discussed.

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A piezoelectric immunosensor for specific capture and enrichment of viable pathogens by quartz crystal microbalance sensor, followed by detection with antibody-functionalized gold nanoparticles

Cited by 108 publications

References 33 publications

Emerging frontiers in detection and control of bacterial biofilms

Emerging frontiers in detection and control of bacterial biofilms

Biosensors for Whole-Cell Bacterial Detection

Noble metal nanoparticles in biosensors: recent studies and applications

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