In this experiment, we tried to measure personality by reaction time (RT) to stimuli of personality trait words. There were interindividual and intraindividual differences in the factors that caused the reaction time to fluctuate. The intraindividual differences for personality trait words were caused by changes due to circumstances for the same participant. The increased stimulus reaction time (sRT) model for simple reaction time was used as an index to indicate personality traits. As a result, participants could be classified into two major hierarchical clusters. The participants in Cluster 1 showed innovative dominance. The participants in Cluster 2 were obedient and conservative. The independent variable was measured by the physiological index using sRT for classify the participants. Participants in Cluster 2 had a reduced stress response to the experiment and showed a tendency to be compliant. Moreover, immediately after the RT measurement session with a laptop computer started, participants in Cluster 1 showed decreased HEG and increased amylase values and had a somewhat negative attitude. The physiological dependent variable were measured by using salivary amylase and hemoencephalography (HEG). And, the psychological dependent variable was the Big Five personality inventory. All of them ware using to verify the participant’s classification. Participants in Cluster 2 had significantly higher conscientiousness than those in Cluster 1. Therefore, we suggest that it is possible to classify personality traits from RT by using sRT based on intraindividual differences.