A Pilot Study of Third-Generation Dual-Source Computed Tomography for the Assessment of Global Dynamic Changes in Left Ventricular Structure and Function in a Porcine Model of Acute Myocardial Infarction

Abstract

BackgroundFirst-generation and second-generation dual-source computed tomography (DSCT) are useful for analyzing left ventricle (LV) structure and function. This pilot study aimed to investigate the feasibility and role of third-generation DSCT for the evaluation of dynamic changes in LV structural and functional characteristics in a Diannan small-ear pig model of acute myocardial infarction (AMI).Material/MethodsThe model of AMI was established by balloon occlusion of the distal third of the left anterior descending (LAD) coronary artery in 14 Diannan small-eared pigs. Third-generation DSCT was performed to observe dynamic changes in LV structure and function before and after AMI was induced, with a follow-up period of 30 days.ResultsThe mean structural measurements at baseline included interventricular septum thickness (8.50±0.90 mm), LV anterior wall thickness (8.40±1.30 mm), LV posterior wall thickness (7.80±1.20 mm), LV end-diastolic dimension (LVEDD) (45.00±4.90 mm), and LV end-systolic dimension (LVESD) (25.90±4.10 mm). The mean functional measurements at baseline included the LV end-diastolic volume (LVEDV) (74.62±13.54 ml), LV end-systolic volume (LVESV) (23.06±7.46 ml), LV ejection fraction (LVEF) (69.29±6.83%), LV mass (86.35±14.02 g), stroke volume (SV) (51.56±9.77 ml), and cardiac output (CO) (4.22±2.14 l/min). Trends of time-dependent changes were observed for LVESV, LVEF, SV, and CO, but not for LVEDV or LV mass.ConclusionsThird-generation DSCT was validated as a tool for assessing dynamic changes in LV global function in a porcine model of AMI.

Copeptin Reflect Left Ventricular Systolic Function at Early Stage of Acute Myocardial Infarction in a Pig Model

“…As shown in Figure 2 , 25 there were significant differences in the 4 parameters of LVEF, LVESV, SV and CO between the control group and the AMI group ( P ⩽ .05). Specifically, 30 days after AMI, LVEF decreased from the baseline value of 69.83% ± 6.09% to 50.33% ± 5.66%, SV decreased from 53.07 ± 6.51 to 40.85 ± 4.97 ml, and LVESV increased from 22.97 ± 4.55 to 40.71 ± 6.42 ml, CO decreased from 5.06 ± 2.42 to 3.82 ± 1.69 l/minutes.…”

Brain structure analysis of different age groups of Diannan small‐ear pigs

“…Digital imaging and communications in medicine (DICOM) images were converted to NIFI format using the Micron software package, and all voxels were expanded 10 times for subsequent processing by SPM12 using Data Processing & Analysis for Brain Imaging (DPABI) software for all structural image data (http://www.fil.ion.ucl.ac.uk/spm/software/spm12/). Using the display function of SPM12, the structural images were converted to the same spatial orientation as the structural image template of the pigs 2 . Whole‐brain images were delineated using ITK‐SNAP (http://www.itksnap.org/pmwiki/pmwiki.php), and regions of interest (ROI) were delineated and their volumes and signal values were measured based on stereograms and histological maps of the pigs 3 .…”

“…At present, mice are still the most widely used model animal, but there are great differences between mice and humans, and there are great limitations in the study of some specific diseases. Nonhuman primates, such as monkeys and gorillas, are the ideal model animals that are closest to human beings 2,3 . However, these animals are not widely used in clinical practice because of their high cost and ethical issues.…”

“…Nonhuman primates, such as monkeys and gorillas, are the ideal model animals that are closest to human beings. 2 , 3 However, these animals are not widely used in clinical practice because of their high cost and ethical issues. Due to the advantages of minimal differences from humans, manageable costs, and no major ethical issues, 4 some researchers have suggested that miniature pigs are the most valuable experimental model animals, except for nonhuman primates.…”

CT imaging character of different brain regions in different ages of Diannan small‐ear pigs

“…Such results not only clarify the heart size of pigs, but also increase the success rate of preclinical research and achieve higher cost-efectiveness (Lipiski M, et al, 2020). Besides this , pigs have also developed rapidly in the establishment of disease models assisted by CT. Thirdgeneration dual-source CT was validated as a tool for assessing dynamic changes in left ventricle global function in a porcine model of acute myocardial infarction (Li W, et al, 2019). Utilize quantitative CT to detect and evaluate the severity of lung injury after successful cardiopulmonary resuscitation in a porcine cardiac arrest model with diferent downtimes.…”