A pilot study to assess oral colonization and pH buffering by the probiotic Streptococcus dentisani under different dosing regimes

Ferrer, Milagros; López‐López, Arantxa; Nicolescu, Teodora; Salavert, Ariana; Méndez, Iago; Cuñé, Jordi; Llena, Carmen; Mira, Álex

doi:10.1007/s10266-019-00458-y

Cited by 27 publications

(28 citation statements)

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“…In the current work, mean values of CFUs per ml of saliva were significantly higher in the probiotic group than those of placebo, both at the end of treatment and at the end of the study. In dental plaque, however, significant differences were only observed at the end of the application period, suggesting that colonization is partially lost after those 2 weeks, which is consistent with a previous pilot study where the probiotic was administered only for a week (Ferrer et al, 2020 ). Our data show that at the end of the treatment period, only 58% of participants in the probiotic group showed colonization, reaching 71% by the end of the study.…”

Section: Discussionsupporting

confidence: 89%

“…To measure total lactic acid and pH of saliva and plaque samples, reflectometry with the RQflex20 Reflectoquant System (Merk) was used, according to manufacturer's instructions and following Helmke et al ( 2009 ) and Ferrer et al ( 2020 ). The equipment was calibrated by the use of standards with known concentrations.…”

Section: Methodsmentioning

confidence: 99%

“…Streptococcus dentisani in saliva and dental plaque samples was quantified by real-time PCR at each study visit. S. dentisani primers targeted the genes for the carbamate kinase ( arcC ), as detailed in Ferrer et al ( 2020 ). Real-time PCR was performed in a LightCycler 480 System with the LightCycler 480 SYBR Green I Master Mix (Roche).…”

Section: Methodsmentioning

confidence: 99%

“…In vitro studies show that S. dentisani is able to inhibit and displace pre-existing cultures of oral pathogens on human gingival cells (Esteban-Fernández et al, 2018 , 2019 ). Recently, a pilot clinical study showed that S. dentisani strain 7746 was able to colonize human dental plaque for at least 2 weeks after topic application with a dental ferule (Ferrer et al, 2020 ). In addition, salivary pH and lactate production improved after 1-week treatment, and a reduction in the counts of the cariogenic organism S. mutans was also observed, but the small sample size in this pilot test (only 12 individuals were studied) prevented a full evaluation of this strain as a probiotic.…”

Section: Introductionmentioning

confidence: 99%

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Topic Application of the Probiotic Streptococcus dentisani Improves Clinical and Microbiological Parameters Associated With Oral Health

Ferrer

López-López

Nicolescu

et al. 2020

Front. Cell. Infect. Microbiol.

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Streptococcus dentisani 7746, isolated from dental plaque of caries-free individuals, has been shown to have several beneficial effects in vitro which could contribute to promote oral health, including an antimicrobial activity against oral pathogens by the production of bacteriocins and a pH buffering capacity through ammonia production. Previous work has shown that S. dentisani was able to colonize the oral cavity for 2–4 weeks after application. The aim of the present work was to evaluate its clinical efficacy by a randomized, double-blind, placebo-controlled parallel group study. Fifty nine volunteers were enrolled in the study and randomly assigned to a treatment or placebo group. The treatment consisted of a bucco-adhesive gel application (2.5 10 9 cfu/dose) with a dental splint for 5 min every 48 h, for a period of 1 month (i.e., 14 doses). Dental plaque and saliva samples were collected at baseline, 15 and 30 days after first application, and 15 days after the end of treatment. At baseline, there was a significant correlation between S. dentisani levels and frequency of toothbrushing. Salivary flow, a major factor influencing oral health, was significantly higher in the probiotic group at day 15 compared with the placebo (4.4 and 3.4 ml/5 min, respectively). In the probiotic group, there was a decrease in the amount of dental plaque and in gingival inflammation, but no differences were observed in the placebo group. The probiotic group showed a significant increase in the levels of salivary ammonia and calcium. Finally, Illumina sequencing of plaque samples showed a beneficial shift in bacterial composition at day 30 relative to baseline, with a reduction of several cariogenic organisms and the key players in plaque formation, probably as a result of bacteriocins production. Only 58% of the participants in the probiotic group showed increased plaque levels of S. dentisani at day 30 and 71% by day 45, indicating that the benefits of S. dentisani application could be augmented by improving colonization efficiency. In conclusion, the application of S. dentisani 7746 improved several clinical and microbiological parameters associated with oral health, supporting its use as a probiotic to prevent tooth decay.

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Section: Discussionsupporting

confidence: 89%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Topic Application of the Probiotic Streptococcus dentisani Improves Clinical and Microbiological Parameters Associated With Oral Health

Ferrer

López-López

Nicolescu

et al. 2020

Front. Cell. Infect. Microbiol.

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“…A method was used based on Hemke et al (2009) and Ferrer et al (2020) 30,31 . In short, undiluted supernatant was used for pH measurements and, for the rest of the measurements, 10 × or higher dilutions were made to obtain a concentration within the detection threshold of the test strips.…”

Section: Methodsmentioning

confidence: 99%

Nitrate as a potential prebiotic for the oral microbiome

Rosier

Buetas

Moya-Gonzálvez

et al. 2020

Sci Rep

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100

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The salivary glands actively concentrate plasma nitrate, leading to high salivary nitrate concentrations (5-8 mM) after a nitrate-rich vegetable meal. Nitrate is an ecological factor that can induce rapid changes in structure and function of polymicrobial communities, but the effects on the oral microbiota have not been clarified. To test this, saliva of 12 healthy donors was collected to grow in vitro biofilms with and without 6.5 mM nitrate. Samples were taken at 5 h (most nitrate reduced) and 9 h (all nitrate reduced) of biofilm formation for ammonium, lactate and pH measurements, as well as 16S rRNA gene Illumina sequencing. Nitrate did not affect biofilm growth significantly, but reduced lactate production, while increasing the observed ammonium production and pH (all p < 0.01). Significantly higher levels of the oral health-associated nitrate-reducing genera Neisseria (3.1 ×) and Rothia (2.9 ×) were detected in the nitrate condition already after 5 h (both p < 0.01), while several caries-associated genera (Streptococcus, Veillonella and Oribacterium) and halitosis-and periodontitis-associated genera (Porphyromonas, Fusobacterium, Leptotrichia, Prevotella, and Alloprevotella) were significantly reduced (p < 0.05 at 5 h and/or 9 h). In conclusion, the addition of nitrate to oral communities led to rapid modulation of microbiome composition and activity that could be beneficial for the host (i.e., increasing eubiosis or decreasing dysbiosis). Nitrate should thus be investigated as a potential prebiotic for oral health.

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Probiotic Interventions for Oral Health

Twetman

Jørgensen

2021

Probiotic Research in Therapeutics

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A pilot study to assess oral colonization and pH buffering by the probiotic Streptococcus dentisani under different dosing regimes

Cited by 27 publications

References 27 publications

Topic Application of the Probiotic Streptococcus dentisani Improves Clinical and Microbiological Parameters Associated With Oral Health

Topic Application of the Probiotic Streptococcus dentisani Improves Clinical and Microbiological Parameters Associated With Oral Health

Nitrate as a potential prebiotic for the oral microbiome

Probiotic Interventions for Oral Health

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