A Pipette-Based Calibration System for Fast-Scan Cyclic Voltammetry with Fast Response Times

Ramsson, Eric S.

doi:10.2144/000114476

Cited by 9 publications

(12 citation statements)

References 36 publications

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“…Animals were killed by cervical dislocation and brains were sectioned in oxygenated, 4°C artificial cerebrospinal fluid (aCSF) at 300 µm thick using a vibratome (Campden Instruments 5100mz-Plus) ( Ferris et al , 2014 ). FSCV was carried out in the lateral, dorsal striatal sections as previously described ( Everett et al , 2022 ; Ferris et al , 2014 ; Lohr et al , 2014 ; Ramsson, 2016 ; Ramsson et al , 2015 ; Yorgason et al , 2011 ).…”

Section: Methodsmentioning

confidence: 99%

“…Carbon fiber glass microelectrodes were constructed using a vacuum to pull carbon fiber through a glass capillary tube, pulled using a horizontal electrode puller, broken, and sealed with paraffin ( Ramsson et al , 2015 ). Microelectrodes were cycled for at least 15 min prior to recording at a frequency of 60 Hz, then cycled at 10 Hz until stable ( Davis et al , 2020 ; Ramsson, 2016 ; Ramsson et al , 2015 ; Takmakov et al , 2010 ). Carbon fiber microelectrodes were calibrated using a pipette-based calibration system by adding a dilute stock DA solution to a buffer and measuring the oxidation and/or reduction ( Ramsson, 2016 ).…”

Section: Methodsmentioning

confidence: 99%

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Developmental exposure to the Parkinson’s disease-associated organochlorine pesticide dieldrin alters dopamine neurotransmission in α-synuclein pre-formed fibril (PFF)-injected mice

Boyd,

Kuhn,

Patterson

et al. 2023

Toxicological Sciences

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Parkinson’s disease (PD) is the fastest-growing neurological disease worldwide, with increases outpacing aging and occurring most rapidly in recently industrialized areas, suggesting a role of environmental factors. Epidemiological, post-mortem, and mechanistic studies suggest that persistent organic pollutants, including the organochlorine pesticide dieldrin, increase PD risk. In mice, developmental dieldrin exposure causes male-specific exacerbation of neuronal susceptibility to 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and synucleinopathy. Specifically, in the α-synuclein (α-syn) pre-formed fibril (PFF) model, exposure leads to increased deficits in striatal dopamine (DA) turnover and motor deficits on the challenging beam. Here, we hypothesized that alterations in DA handling contribute to the observed changes and assessed vesicular monoamine transporter 2 (VMAT2) function and DA release in this dieldrin/PFF 2-hit model. Female C57BL/6 mice were exposed to 0.3 mg/kg dieldrin or vehicle every 3 days by feeding, starting at 8 weeks of age and continuing throughout breeding, gestation, and lactation. Male offspring from independent litters underwent unilateral, intrastriatal injections of α-syn PFFs at 12 weeks of age, and vesicular 3H-DA uptake assays and fast-scan cyclic voltammetry were performed 4 months post-PFF injection. Dieldrin-induced an increase in DA release in striatal slices in PFF-injected animals, but no change in VMAT2 activity. These results suggest that developmental dieldrin exposure increases a compensatory response to synucleinopathy-triggered striatal DA loss. These findings are consistent with silent neurotoxicity, where developmental exposure to dieldrin primes the nigrostriatal striatal system to have an exacerbated response to synucleinopathy in the absence of observable changes in typical markers of nigrostriatal dysfunction and degeneration.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Developmental exposure to the Parkinson’s disease-associated organochlorine pesticide dieldrin alters dopamine neurotransmission in α-synuclein pre-formed fibril (PFF)-injected mice

Boyd,

Kuhn,

Patterson

et al. 2023

Toxicological Sciences

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show abstract

“…To improve the seal around the carbon fibre, CFMs tips were immersed in melted paraffin, and extra coating was removed with xylene 107 . CFMs were calibrated after each recording by quantifying DA at known concentrations (1.25, 2.5, 5 μM) as previously described 108 .…”

Section: Fast-scan Cyclic Voltammetry (Fscv)mentioning

confidence: 99%

Dysregulated acetylcholine-mediated dopamine neurotransmission in the eIF4E Tg mouse model of autism spectrum disorders

Carbonell-Roig,

Aaltonen,

Cartocci

et al. 2024

Preprint

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Autism Spectrum Disorders (ASD) consist of diverse neurodevelopmental conditions where core behavioral symptoms are critical for diagnosis. Altered dopamine neurotransmission in the striatum has been suggested to contribute to the behavioral features of ASD. Here, we examine dopamine neurotransmission in a mouse model of ASD characterized by elevated expression of the eukaryotic initiation factor 4E (eIF4E), a key regulator of cap-dependent translation, using a comprehensive approach that encompasses genetics, behavior, synaptic physiology, and imaging. The results indicate that increased eIF4E expression leads to behavioral inflexibility and impaired striatal dopamine release. The loss of normal dopamine neurotransmission is due to a defective nicotinic receptor signaling that regulates calcium dynamics in dopaminergic axons. These findings reveal an intricate interplay between eIF4E, DA neurotransmission, and behavioral flexibility, provide a mechanistic understanding of ASD symptoms and offer a foundation for targeted therapeutic interventions.

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“…DA experiments were performed in vitro using a pipette-based calibration configuration as previously described [18]. Concentrations of 50 nM, 100 nM, 200 nM, 500 nM, and 1 μM DA were used for the standard curve.…”

Section: Da and Aa Detectionmentioning

confidence: 99%

Enhancement of fast scan cyclic voltammetry detection of dopamine with tryptophan-modified electrodes

2020

Self Cite

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Fast scan cyclic voltammetry (FSCV) allows for real-time analysis of phasic neurotransmitter levels. Tryptophan (TRP) is an aromatic amino acid responsible for facilitating electron transfer kinetics in oxidoreductase enzymes. Previous work with TRP-modified electrodes showed increased sensitivity for cyclic voltammetry detection of dopamine (DA) when used with slower scan rates (0.05 V/s). Here, we outline an in vitro proof of concept for TRP-modified electrodes in FSCV detection of DA, and decreased sensitivity for ascorbic acid (AA). TRP-modified electrodes had a limit of detection (LOD) for DA of 2.480 ± 0.343 nM compared to 8.348 ± 0.405 nM for an uncoated electrode. Selectivity for DA/ascorbic acid (AA) was 1.107 ± 0.3643 for uncoated and 15.57 ± 4.184 for TRP-modified electrodes. Additionally, these TRP-modified electrodes demonstrated reproducibility when exposed to extended cycling. TRP-modified electrodes will provide an effective modification to increase sensitivity for DA.

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A Pipette-Based Calibration System for Fast-Scan Cyclic Voltammetry with Fast Response Times

Cited by 9 publications

References 36 publications

Developmental exposure to the Parkinson’s disease-associated organochlorine pesticide dieldrin alters dopamine neurotransmission in α-synuclein pre-formed fibril (PFF)-injected mice

Developmental exposure to the Parkinson’s disease-associated organochlorine pesticide dieldrin alters dopamine neurotransmission in α-synuclein pre-formed fibril (PFF)-injected mice

Dysregulated acetylcholine-mediated dopamine neurotransmission in the eIF4E Tg mouse model of autism spectrum disorders

Enhancement of fast scan cyclic voltammetry detection of dopamine with tryptophan-modified electrodes

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