A Plaque Assay for Nuclear Polyhedrosis Viruses using a Solid Overlay

Brown, Martha; Faulkner, P.

doi:10.1099/0022-1317-36-2-361

Cited by 194 publications

(93 citation statements)

References 2 publications

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“…Purified AcNPV genomic DNA was obtained from Invitrogen. Procedures for the culture of Sf9 ceils and the growth and plaque assay of AcNPV have been described previously (Knudson & Tinsley, 1974;Brown & Faulkner, 1977).…”

Section: Methodsmentioning

confidence: 99%

Recombinant baculovirus-expressed NS3 proteinase of hepatitis C virus shows activity in cell-based and in vitro assays

Overton

McMillan

Gillespie

et al. 1995

Journal of General Virology

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Recombinant baculoviruses have been constructedwhich express the hepatitis C virus (HCV) NS3 proteinase and its substrates in insect cells. The expressed proteinase has been shown to carry out transcleavage at the NS3/4A, NS4A/4B, NS4B/5A and NS5A/5B junctions in a cell-based assay. When assayed in a cell-free system using in vitro translated substrates, the proteinase could perform trans-processing of the NS4A/4B and NS5A/5B junctions, but only when coexpressed with NS4A, either as an NS3-4A precursor or by co-infection of cells with NS3-and NS4A-expressing recombinant baculoviruses. Possible reasons for the absolute requirement of the NS3 proteinase for NS4A in vitro are discussed.

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Section: Methodsmentioning

confidence: 99%

Recombinant baculovirus-expressed NS3 proteinase of hepatitis C virus shows activity in cell-based and in vitro assays

Overton

McMillan

Gillespie

et al. 1995

Journal of General Virology

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“…AcNPV and recombinant virus stocks were grown and assayed in confluent monolayers of S. frugiperda cells in medium containing 10~ foetal bovine serum according to the procedures described by Brown & Faulkner (1977). On occasion virus stocks were obtained using spinner cultures of S. frugiperda cells.…”

Section: Methodsmentioning

confidence: 99%

Baculovirus Expression Vectors: the Requirements for High Level Expression of Proteins, Including Glycoproteins

Matsuura¹,

Possee²,

Overton³

et al. 1987

Journal of General Virology

670

374

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“…The S. frugiperda cell line IPLB-Sf-21-AE (Sf21) and the B. mori cell line BraN were used and maintained in tissue culture flasks in TC-10 medium containing 10% foetal calf serum (FCS) (Brown & Faulkner, 1977 AcMNPV was kindly supplied by Professor David Bishop (NERC Institute of Virology and Environmental Microbiology, Oxford, U.K.) and BmNPV was isolated in our laboratory by plaque purification. AcMNPV and BmNPV genomic DNA was extracted and purified from viral particles according to the method of Summers & Smith (1987).…”

mentioning

confidence: 99%

Foreign gene expression by a baculovirus vector with an expanded host range

Mori

Nakazawa

Shirai

et al. 1992

Journal of General Virology

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A nuclear polyhedrosis virus (NPV) (Baculoviridae)-based gene expression system was improved by DNA recombination. The BmN cell line established from Bombyx mori and the Sf21 cell line established from Spodopterafrugiperda are non-permissive for Autographa californica multicapsid NPV (AcMNPV) and B. mori NPV (BmNPV) replication, respectively. After cotransfection of AcMNPV DNA and BamHI-digested BmNPV DNA into Sf21 cells, progeny viruses were isolated by plaque purification on BmN cell monolayers and the host specificity of one viral isolate was analysed. The virus had a wider host range, and replicated and produced polyhedra in Sf21 cells, BmN cells and larvae of the silkworm, B. morL DNA restriction endonuclease analysis showed that the isolate was a hybrid of AcMNPV and BmNPV. Using

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A Plaque Assay for Nuclear Polyhedrosis Viruses using a Solid Overlay

Cited by 194 publications

References 2 publications

Recombinant baculovirus-expressed NS3 proteinase of hepatitis C virus shows activity in cell-based and in vitro assays

Recombinant baculovirus-expressed NS3 proteinase of hepatitis C virus shows activity in cell-based and in vitro assays

Baculovirus Expression Vectors: the Requirements for High Level Expression of Proteins, Including Glycoproteins

Foreign gene expression by a baculovirus vector with an expanded host range

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