A plasma ion bombardment process enabling reagent-free covalent binding of multiple functional molecules onto magnetic particles

Tran, Clara; Craggs, Michael; Smith, Lorraine P.; Stanley, Keith; Bilek, Marcela M.M.; McKenzie, David R.

doi:10.1016/j.msec.2018.12.106

Cited by 8 publications

(12 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The signal was still observable after overnight incubation in stripping buffer at 37 °C (not shown). This strong attachment of Abs is consistent with covalent bonds of biomolecules on PIII‐treated MPs reported in previous work …”

Section: Resultssupporting

confidence: 92%

“…It was especially prominent with emission filters for wavelengths of 500 to 550 nm (Figure A, upper panel). Third, the treated MPs became more hydrophilic and dispersed well in water even after storage for up to 18 hours, consistent with earlier observations . PIII treatment is known to increase surface energy and hydrophilicity of polystyrene.…”

Section: Resultssupporting

confidence: 90%

“…Previously, we demonstrated the attachment of an oligonucleotide to PIII-treated magnetic particles. 9 Here we extend the work to describe the immobilization of poly A-tailed siRNAs on PIII-treated MPs. An excess of siRNA molecules was incubated with UT or PIII-treated MPs overnight in a pH4 acetate buffer.…”

Section: Covalent Attachment Of a Tailed Sirna To Piii-treated Mpsmentioning

confidence: 83%

“…However, it is expected that the Abs remain native, as previous work showed that immobilized horseradish peroxidase remains functional, indicating that the radicals tether the protein without denaturing it. The more hydrophilic surface of the PIII‐treated MPs also helps to retain native protein confirmation . Radical binding is also not specific and may occur at different sites of the Ab leaving the antigen binding site exposed.…”

Section: Discussionmentioning

confidence: 99%

“…Future studies will investigate target binding abilities of the immobilized Abs. Additional advantages of the low‐temperature PIII process are the inherent particle sterilization and the ability to attach multiple biomolecules simultaneously …”

Section: Discussionmentioning

confidence: 99%

See 4 more Smart Citations

Covalent binding of molecules to plasma immersion ion implantation‐activated microparticles for delivery into cells

Kruse

Tran

Zandwijk

et al. 2020

Engineering Reports

Self Cite

View full text Add to dashboard Cite

Delivery of biomolecules to target cells is an essential step in gene therapy, targeted drug delivery, and cell imaging, and can be achieved by encapsulation or conjugation to micro‐ or nanoparticles. For successful systemic delivery, these complexes must be stable under physiological conditions and prevent leakage of the cargo. Covalent binding of the active agent to a carrier is one‐way to facilitate this stability but frequently requires several steps. Here we show that plasma immersion ion implantation (PIII) treatment can activate the surface of paramagnetic polystyrene microparticles (MPs), increasing autofluorescence and allowing conjugation of biological molecules in a single‐step. Using PIII‐activated MPs, we demonstrate covalent binding of therapeutically relevant payloads including antibodies (Abs) and small‐interfering RNAs (siRNAs). The PIII‐activated magnetic particles were actively taken up by the cells, were nontoxic, and could be visualized due to increased autofluorescence induced by the PIII treatment. Ab and siRNA were effectively conjugated simultaneously to the active surface, and these functionalized MPs were also taken up by cells. This simple PIII‐based activation system has the potential to be applied to many different therapeutic approaches.

show abstract

Section: Resultssupporting

confidence: 92%

Section: Resultssupporting

confidence: 90%

Section: Covalent Attachment Of a Tailed Sirna To Piii-treated Mpsmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Covalent binding of molecules to plasma immersion ion implantation‐activated microparticles for delivery into cells

Kruse

Tran

Zandwijk

et al. 2020

Engineering Reports

Self Cite

View full text Add to dashboard Cite

show abstract

Plasma immersion ion‐implanted 3D‐printed PEEK bone implants: In vivo sheep study shows strong osseointegration

Kruse

Lewin

Suchowerska

et al. 2022

Plasma Processes & Polymers

Self Cite

View full text Add to dashboard Cite

Adhesion of regenerating bone tissue to orthopaedic implants is critical to their long‐term success. Titanium has excess rigidity, interferes with imaging, and complicates radiation treatment. Poly(ether‐ether‐ketone) (PEEK) is a preferred material, but is limited by biological inertness. We show that plasma immersion ion implantation (PIII) imparts osteoadhesion on 3D printed PEEK using in vitro pull‐off tests and in vivo torque tests in a sheep scapula model. PIII‐PEEK prints incubated with bone cells in vitro show 9–14 times higher pull‐off strength, compared to untreated implants. Treated implants in vivo require seven times higher torque for release from the sheep scapula. We propose PIII‐PEEK as a scaffold material with tensile properties analogous to collagen, the fibrillar component of bone, to produce fracture‐tough fully integrating scaffolds.

show abstract

An Ultrasensitive Fluorescence Immunoassay Based on Magnetic Separation and Upconversion Nanoparticles as Labels for the Detection of Chloramphenicol in Animal-Derived Foods

et al. 2020

View full text Add to dashboard Cite

A plasma ion bombardment process enabling reagent-free covalent binding of multiple functional molecules onto magnetic particles

Cited by 8 publications

References 24 publications

Covalent binding of molecules to plasma immersion ion implantation‐activated microparticles for delivery into cells

Covalent binding of molecules to plasma immersion ion implantation‐activated microparticles for delivery into cells

Plasma immersion ion‐implanted 3D‐printed PEEK bone implants: In vivo sheep study shows strong osseointegration

An Ultrasensitive Fluorescence Immunoassay Based on Magnetic Separation and Upconversion Nanoparticles as Labels for the Detection of Chloramphenicol in Animal-Derived Foods

Contact Info

Product

Resources

About