A plasmid vector that directs hyperproduction of recombinant proteins in the thermophiles Geobacillus species

Kurashiki, Ryota; Mizuno, Tatsuki; Murata, Kurumi; Ohshiro, Takashi; Suzuki, Hirokazu

doi:10.1007/s00792-019-01142-3

Cited by 7 publications

(2 citation statements)

References 35 publications

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“…The Gram-positive bacterium Geobacillus and Parageobacillus are ideal candidates as microbial cell factories for sustainable production of industrially important chemicals due to their ability to grow at very high temperatures and can utilise a diverse range of substrates as an energy and carbon source, such as lignocellulose and crude oil(Hussein et al, 2015; Khaswal et al, 2022). Previous studies have expanded the available genetic tools for engineering different species of Geobacillus , which include vectors and libraries of basic parts(Bartosiak-Jentys et al, 2013; Koyama et al, 2022; Kurashiki et al, 2020; Madika et al, 2022; Pogrebnyakov et al, 2017; Reeve et al, 2016; Taylor et al, 2008). However, a toolkit for standardised modular and hierarchical assembly is not available.…”

Section: Resultsmentioning

confidence: 99%

SubtiToolKit - a bioengineering kit forBacillus subtilisand Gram-positive bacteria

Caro-Astorga,

Rogan,

Malci

et al. 2024

Preprint

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SummaryBuilding DNA constructs of increasing complexity is key to synthetic biology. Golden Gate methods led to the creation of cloning toolkits - collections of modular standardized DNA parts hosted on hierarchic plasmids, developed for yeast, plants, Gram-negative bacteria, and human cells. However, Gram-positive bacteria have been neglected.Bacillus subtilisis a Gram-positive model organism and a workhorse in the bioindustry. Here, we present the SubtiToolKit, a high- efficiency cloning toolkit forB. subtilisand Gram-positive bacteria. Its design permits DNA constructs for transcriptional units, operons, knock-in and knock-out applications. It contains libraries of promoters, RBSs, fluorescent proteins, protein tags, terminators, genome integration parts, a no-leakage genetic device to control the expression of toxic products duringE. coliassembly, and a toolbox for industrially relevant strains ofGeobacillusandParageobacillusas an example of SubtiToolKit versatility for other Gram-positive bacteria and its future perspective as a reference toolkit.

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Section: Resultsmentioning

confidence: 99%

SubtiToolKit - a bioengineering kit forBacillus subtilisand Gram-positive bacteria

Caro-Astorga,

Rogan,

Malci

et al. 2024

Preprint

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“…Since the amount of coloring matter or dye present in natural dye-bearing materials is relatively low, along with other plant and animal compounds like waterinsoluble fibers, carbohydrates, protein, chlorophyll, and tannins, among others, extraction is a crucial step both in the production of purified natural dyes as well as in the processing of raw dye-bearing materials [34]. Before using an extraction procedure, the type and solubility characteristics of the coloring components must be studied [35].…”

Section: Natural Dyes Production and Extraction Techniquesmentioning

confidence: 99%

Ecological Applications of Enzymes in Plants Based Textile Dyeing

Haddar,

Adeel,

Salman

et al. 2023

Dyes and Pigments - Insights and Applications

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Biotechnology has a foremost role in the textile industry by enhancing ecofriendly, cost-effective, and energy-efficient manufacturing processes. The use of enzymatic biotechnology is one of the sustainable newly developed state-of-the-art processes for textile processing. To reduce the use of toxic and hazardous chemicals, enzymes have been proposed as one of the finest promising alternatives. Many enzymes have been used widely in textile processes such as lipase, laccase, pectinase, cellulase, catalase, amylase, and protease. The enzymatic use in the textile industry is very promising because they produce top-class goods, and give way to the reduction of water, time, and energy. The increasing demand for natural dyes especially with the incorporation of enzymes makes process more sustainable and eco-friendlier to suppress the toxicity of synthetic dyes. In the first part of the chapter, particular attention has been given to the source and extraction of natural dyes. In the second part of the chapter, different enzymes and their possible roles in the textile industry have been discussed. It is expected that this chapter will provide an innovative direction to the academic researchers, the community of textile and traders as well as artisans who are working in the area of biotechnological applications for the betterment of textile processing.

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Development of a thermophilic host–vector system for the production of recombinant proteins at elevated temperatures

Kurashiki,

Koyama,

Sakaguchi

et al. 2023

Appl Microbiol Biotechnol

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A plasmid vector that directs hyperproduction of recombinant proteins in the thermophiles Geobacillus species

Cited by 7 publications

References 35 publications

SubtiToolKit - a bioengineering kit forBacillus subtilisand Gram-positive bacteria

SubtiToolKit - a bioengineering kit forBacillus subtilisand Gram-positive bacteria

Ecological Applications of Enzymes in Plants Based Textile Dyeing

Development of a thermophilic host–vector system for the production of recombinant proteins at elevated temperatures

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