A plastic container for rearing insects on artificial diets

Singh, Pritam; Surrey, Michael

doi:10.1080/03014223.1980.10423799

Cited by 5 publications

(1 citation statement)

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“…If a phagostimulant is needed, dried and ground food plants and/or sucrose may be mixed into the diet (Morton 1979). When rearing lepidopteran larvae on artificial diets, specific containers have to be used (Singh and Surrey 1980;Hansen and Zethner 1979;Skovmand and Christoffersen 1994;Davis et al 1990).…”

Section: Breeding On Artificial Dietsmentioning

confidence: 99%

Selection of non-target Lepidoptera species to test Bt maize effects in the laboratory: which species and how to breed them?

Lang¹,

Dolek²,

Lee³

et al. 2020

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Bt maize targeting Lepidopteran pests poses potential risks for non-target (NT) butterflies and moths which are addressed in the environmental risk assessment of genetically modified crop plants. For this purpose, eco-toxicological tests are often conducted with specific NT species in the laboratory in order to assess possible adverse effects. As only a limited number of surrogate species can be addressed, the choice of focal species to be tested is an important decision. However, practical and standardised selection procedures have hardly been developed and applied for NTLepidoptera, so far. Here, we present a transparent and systematic selection process of suitable test species for Germany, involving selection criteria such as exposure to Bt maize, habitat range and laboratory maintenance of the species. As a result, we compiled a list of 15 lepidopteran species particularly appropriate for testing the adverse effects of Bt maize in the laboratory. In addition, we collected and reviewed published reports for breeding methods of Lepidoptera, which provides essential information on maintaining lab stocks of NTLepidoptera. The presented selection procedure allows focusing on the relevant test species in a transparent and reproducible way, and supplies the breeding knowledge required to breed and maintain them, which will be of great utility for the future assessment on possible risks of Bt maize cultivation to non-target Lepidoptera.

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Section: Breeding On Artificial Dietsmentioning

confidence: 99%

Selection of non-target Lepidoptera species to test Bt maize effects in the laboratory: which species and how to breed them?

Lang¹,

Dolek²,

Lee³

et al. 2020

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A general purpose laboratory diet mixture for rearing insects

Singh¹

1983

Int. J. Trop. Insect Sci.

170

119

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A rearing method forHeliothis armiger confertus, and its use for production of the parasitoidApanteles kazakand two insect viruses

Singh¹,

Surrey²,

Burgess³

et al. 1982

New Zealand Journal of Zoology

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Heliothis armiger confertus (Walker) (Lepidoptera: Noctuidae) was reared on an artificial diet in the laboratory. Larvae and pupae were reared at 25±1°C and 18:6 (LID) photoperiod, and adults were mated at an average of 20°C under natural light conditions. The average generation time was 38 days, with larval period 17.3 days, prepupal period 3.6 days, and pupal period 14.5 days. Other data are: average pupal weight -'( 401.5 mg, o 392.0 mg; average survival to pupa 84.9%; average survival to adult 65.4%; pre-oviposition period 2.7 days; fecundity 973 ± 121 eggs per female; adult lifespan approximately 12 days. The parasitoid Apanteles kazak (Telenga) (Hymenoptera: Braconidae) was reared for several generations using H. a. confertus larvae as hosts. It took, on average, 19.3 days from oviposition to cocoon formation, and 26.8 and 29.0 days for adult male and female emergence respectively. Only 25.9% of the host larvae produced parasite cocoons, and 74.1 % of these produced adult parasitoids. Two Heliothis viruses were produced, with maximum yields for granulosis virus of 40 mg per larva and for nuclear polyhedrosis virus of 11.5 mg per larva.

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A plastic container for rearing insects on artificial diets

Cited by 5 publications

References 0 publications

Selection of non-target Lepidoptera species to test Bt maize effects in the laboratory: which species and how to breed them?

Selection of non-target Lepidoptera species to test Bt maize effects in the laboratory: which species and how to breed them?

A general purpose laboratory diet mixture for rearing insects

A rearing method forHeliothis armiger confertus, and its use for production of the parasitoidApanteles kazakand two insect viruses

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