A polyA aptamer-based label-free colorimetric biosensor for the detection of kanamycin in human serum

Misuse of antibiotics has recently been considered a global issue because of its harmful effects on human health. Since conventional methods have numerous limitations, it is necessary to develop fast, simple, sensitive, and reproducible methods for the detection of antibiotics. Among numerous recently developed methods, aptasensors are fascinating because of their good specificity, sensitivity and selectivity. These kinds of biosensors combining aptamer with colorimetric applications of gold nanoparticles to recognize small molecules are becoming more popular owing to their advantageous features, for example, low cost, ease of use, on-site analysis ability using naked eye and no prerequisite for modern equipment. In this review, we have highlighted the recent advances and working principle of gold nanoparticles based colorimetric aptasensors as promising methods for antibiotics detection in different food and environmental samples (2011–2020). Furthermore, possible advantages and disadvantages have also been summarized for these methods. Finally, the recent challenges, outlook, and promising future perspectives for developing novel aptasensors are also considered.

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“…On KAN addition, aptamer dissociated from AuNPs surface to bind KAN. The method is used to detect KAN concentration in serum clinically [ 68 ].…”

Section: Gold Nanoparticles (Aunps) Based Colorimetric Aptasensorsmentioning

confidence: 99%

“…Does not require expensive instruments or a skilled user. Accurate and reliable clinical applications for KAN detection in serum [ 68 ].…”

Section: Figurementioning

confidence: 99%

Advances in Gold Nanoparticles-Based Colorimetric Aptasensors for the Detection of Antibiotics: An Overview of the Past Decade

Zahra

Ali

et al. 2021

Nanomaterials

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“…The binding of kanamycin to the aptamer liberated the polyA-tailed aptamer from the Au NPs surface, causing the aggregation of Au NPs by the PDDA. The colorimetric assay based on PDDA-induced aggregation lowered the LOD to 0.05 nM [53].…”

Section: Based On Target-induced Aggregation Of Metal Nanoparticlesmentioning

confidence: 99%

Recent Advances in Optical Detection of Aminoglycosides

Zhou

Cao

2020

Applied Sciences

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Aminoglycosides (AGs) are broad-spectrum antibiotics used in both human infection and animal medicine. The overuse of AGs causes undesirable residues in food, leading to serious health problems due to food chain accumulation. In recent years, various methods have been developed to determine AGs in food. Among these methods, fluorescent (FL), colorimetric and chemiluminescent (CL) optical methods possess advantages such as their simple instrumentation, low cost, simple operation, feasibility of realizing visualization, and smartphone imaging. This mini-review summarizes optical assays for the detection of AGs in food developed in recent years. The detection principles for different categories are discussed. Then, the amplification techniques for the ultrasensitive detection of AGs are introduced. We also discuss multiplex methods for the simultaneous detection of AGs. Finally, the challenges and future prospects are discussed in the Conclusions and Perspectives section.

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“…The color change is then observed. By coupling with polyA aptamer, and poly diallyl dimethyl ammonium chloride (an D r a f t aggregating agent), AuNPs have more sensitive response to the trace level of kanamycin (Shayesteh and Khosroshahi, 2020).…”

Section: Metal Nanoparticlesmentioning

confidence: 99%

Progress of optical biosensors for analyzing pathogens and organic pollutants in water since 2015

et al. 2022

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Water contamination by pathogens and organic pollutants is one of the major environmental problems that risk human health. Climate change with extreme weather can promote their prevalence in waters. Environmental monitoring of these pollutants in a fast, continuous, and accurate manner is of increasing demand, especially under the climate change context, but is challenged by their ubiquity and trace concentrations. Optical biosensing is one of the desired solutions owing to its rapid and accurate detection with high sensitivity. Principally, an optical biosensor recognizes these bioactive toxins and contaminants by tailored bioreceptors (e.g., aptamer, enzyme, and cells) and transduces the biological response to optical signals. Research efforts have been made on tailoring bioreceptors and enhancing signal transducing by nanoparticles. This study comprehensively reviewed the mechanisms of optical biosensing and the recent development of bioreceptors and nanomaterials on the enhancement for the rapid, easy, and accurate analysis of emerging contaminants in water. The advantages and challenges on sensitivity, selectivity, and durability of biosensors were discussed along with the opportunities and development strategies.

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A polyA aptamer-based label-free colorimetric biosensor for the detection of kanamycin in human serum

Abstract: A novel colorimetric assay for the sensitive detection of kanamycin (KAN) is described.

Cited by 28 publications

References 40 publications

Advances in Gold Nanoparticles-Based Colorimetric Aptasensors for the Detection of Antibiotics: An Overview of the Past Decade

Advances in Gold Nanoparticles-Based Colorimetric Aptasensors for the Detection of Antibiotics: An Overview of the Past Decade

Recent Advances in Optical Detection of Aminoglycosides

Progress of optical biosensors for analyzing pathogens and organic pollutants in water since 2015

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