A polyA DNA probe-based ultra-sensitive and structure-distinguishable electrochemical biosensor for the analysis of RNAi transgenic maize

Abstract

Since the application of RNA interference (RNAi) is rapidly developing in GMO technology, accurate and sensitive detection of functional RNA molecules was urgently needed, for the safety and function assessment...

Triblock probe-polyA-probe electrochemical interfacial engineering for the sensitive analysis of RNAi plants

“…The design of the positions of RPs and spacers was mainly based on a reported paper of our group. 12…”

“…The design of the positions of RPs and spacers was mainly based on a reported paper of our group. 12 Subsequently, biotin-HRP combined onto the reporter probes and catalyzed a detectable current signal. As shown in Fig.…”

“…Reported non-PCR methods usually need a very long hybridization time 11 or the assistance of amplification strategies to improve sensitivity. 12 Thus, the development of novel RNA detection methods with improved sensitivity and fast speed is now an urgent need.…”

Empowering Electrochemical Biosensing through Nanostructured or Multifunctional Nucleic Acid or Peptide Biomaterials

“…PolyA probes-based electrochemical bioplatforms have been reported to analyze bacterial 16S rRNA, [30] asymmetric PCR amplicons from bacterial genomic DNA, [31] and transgenederived long RNA. [32] These three bioplatforms involved sandwich hybridization formats assisted by the use of one or multiple reporter probes, enzymatic labeling with HRP and chronoamperometric detection with the 3,3′,5,5′-tetramethylbenzidine (TMB)/H 2 O 2 system. [30][31][32]…”

“…[32] These three bioplatforms involved sandwich hybridization formats assisted by the use of one or multiple reporter probes, enzymatic labeling with HRP and chronoamperometric detection with the 3,3′,5,5′-tetramethylbenzidine (TMB)/H 2 O 2 system. [30][31][32]…”