A polyaniline–zeolite nanocomposite material based acetylcholinesterase biosensor for the sensitive detection of acetylcholine and organophosphates

Abstract

In this work, a nanoporous polyaniline-nanocrystalline zeolite organic-inorganic hybrid material was synthesized. An acetylcholinesterase electrochemical biosensor based on a polyaniline-zeolite nanocomposite material was fabricated for the sensitive detection of neurotransmitter acetylcholine and the resultant biosensor was further employed for the detection of toxic organophosphate pesticides. The results demonstrate that the polyaniline-nanocrystalline zeolite based acetylcholinesterase biosensor exhibited much higher activity when compared to conventional polyaniline. The nanoporous polyanilinenanocrystalline zeolite based biosensor exhibited good stability, sensitivity, linear range, and limit of detection. The analytical performance of the developed biosensor was demonstrated in the determination of these pesticides in different real samples (apple, cabbage, tap water, and river water) with satisfactory recovery.

Electrochemical Sensor Platforms Based on Nanostructured Metal Oxides, and Zeolite‐Based Materials

“…[130] The nanoporous polyaniline-nanocrystalline zeolite-based biosensor exhibited excellent stability, sensitivity, linear range (1 ppb-1000 ppb for monocrotophos and 3 ppb-1000 ppb for dichlorvos), and limit of detection (0.1 ppb for monocrotophos and 0.2 ppb for dichlorvos). [130] The analytical efficiency of the created biosensor was demonstrated in the determination of these pesticides in different real samples (apple, cabbage, tap water, and river water) with acceptable recovery. [130] Rajendra and co-authors have developed unique electrochemical biosensor based on nanocrystalline titanosilicate (Nano-TS-1) with the covalent immobilization of the enzyme acetylcholinesterase for the ultra-trace detection of hazardous organophosphate pesticides such as monocrotophos, parathion-methyl, dichlorvos, and malathion ( Figure 9).…”

“…The enzyme AChE was immobilized on a PANI‐Nano‐ZSM‐5 matrix making use of physical entrapment approach. The PANI‐Nano‐ZSM‐5‐based acetylcholinesterase biosensor displayed higher activity when compared to standard PANI based acetylcholinesterase biosensor . The nanoporous polyaniline‐nanocrystalline zeolite‐based biosensor exhibited excellent stability, sensitivity, linear range (1 ppb–1000 ppb for monocrotophos and 3 ppb–1000 ppb for dichlorvos), and limit of detection (0.1 ppb for monocrotophos and 0.2 ppb for dichlorvos) .…”

“…The PANI‐Nano‐ZSM‐5‐based acetylcholinesterase biosensor displayed higher activity when compared to standard PANI based acetylcholinesterase biosensor . The nanoporous polyaniline‐nanocrystalline zeolite‐based biosensor exhibited excellent stability, sensitivity, linear range (1 ppb–1000 ppb for monocrotophos and 3 ppb–1000 ppb for dichlorvos), and limit of detection (0.1 ppb for monocrotophos and 0.2 ppb for dichlorvos) . The analytical efficiency of the created biosensor was demonstrated in the determination of these pesticides in different real samples (apple, cabbage, tap water, and river water) with acceptable recovery …”

“…A linear range for paraoxon (OP) was obtained in the range of 1–26 nM with a LOD of 1 pM . Rajendra and co‐authors have established an acetylcholinesterase electrochemical biosensor based on a polyaniline‐zeolite nanocomposite material for the sensitive detection of poisonous organophosphate pesticides (monocrotophos and dichlorvos) (Figure ) . The enzyme AChE was immobilized on a PANI‐Nano‐ZSM‐5 matrix making use of physical entrapment approach.…”

“…[129] Rajendra and co-authors have established an acetylcholinesterase electrochemical biosensor based on a polyaniline-zeolite nanocomposite material for the sensitive detection of poisonous organophosphate pesticides (monocrotophos and dichlorvos) ( Figure 8). [130] The enzyme AChE was immobilized on a PANI-Nano-ZSM-5 matrix making use of physical entrapment approach. The PANI-Nano-ZSM-5-based acetylcholinesterase biosensor displayed higher activity when compared to standard PANI based acetylcholinesterase biosensor.…”