A polyherbal formulation attenuates hyperoxaluria-induced oxidative stress and prevents subsequent deposition of calcium oxalate crystals and renal cell injury in rat kidneys

Bodakhe, Kiran S.; Namdeo, Kamta Prasad; Patra, Kartik Chandra; Machwal, Lalit; Pareta, Surendra K.

doi:10.1016/s1875-5364(13)60085-0

Cited by 11 publications

(9 citation statements)

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“…Chronic EG exposure leads to hyperoxaluria, renal crystal deposition, oxidative stress and impaired kidney function [ 18 – 22 ]. Given the increasing use of STS in humans [ 23 – 25 ] and its reported beneficial effects in calcifying/crystallizing conditions [ 11 ], we aimed to investigate the effect of STS on EG-induced crystal burden and renal dysfunction.…”

Section: Discussionmentioning

confidence: 99%

Sodium Thiosulfate Ameliorates Oxidative Stress and Preserves Renal Function in Hyperoxaluric Rats

et al. 2015

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BackgroundHyperoxaluria causes crystal deposition in the kidney, which leads to oxidative stress and to injury and damage of the renal epithelium. Sodium thiosulfate (STS, Na2S2O3) is an anti-oxidant, which has been used in human medicine for decades. The effect of STS on hyperoxaluria-induced renal damage is not known.MethodsHyperoxaluria and renal injury were induced in healthy male Wistar rats by chronic exposure to ethylene glycol (EG, 0.75%) in the drinking water for 4 weeks. The treatment effects of STS, NaCl or Na2SO4 were compared. Furthermore, the effects of STS on oxalate-induced oxidative stress were investigated in vitro in renal LLC-PK1 cells.ResultsChronic EG exposure led to hyperoxaluria, oxidative stress, calcium oxalate crystalluria and crystal deposition in the kidneys. Whereas all tested compounds significantly reduced crystal load, only STS-treatment maintained tissue superoxide dismutase activity and urine 8-isoprostaglandin levels in vivo and preserved renal function. In in vitro studies, STS showed the ability to scavenge oxalate-induced ROS accumulation dose dependently, reduced cell-released hydrogen peroxide and preserved superoxide dismutase activity. As a mechanism explaining this finding, STS was able to directly inactivate hydrogen peroxide in cell-free experiments.ConclusionsSTS is an antioxidant, which preserves renal function in a chronic EG rat model. Its therapeutic use in oxidative-stress induced renal-failure should be considered.

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Section: Discussionmentioning

confidence: 99%

Sodium Thiosulfate Ameliorates Oxidative Stress and Preserves Renal Function in Hyperoxaluric Rats

et al. 2015

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“…Published data show that among the 8 different ingredients of this herbal agent, alkaloids, rosmarinic acid, flavonoids [27], apigenin, luteolin [28], ekvizetonin saponin [29], and essential oils [32] are well known for their potent antioxidant as well as anti-inflammatory effects. Regarding the mechanism of action, studies have clearly shown that the above-mentioned active ingredients may reduce the permeability of kidney capillaries, dilate kidney blood vessels and ureters, restore the tubular epithelial function, induce an osmotic effect, inhibit synthesis and activation of inflammatory mediators, and thus prevent inflammatory alterations as well as crystal formation and growth [22,[24][25][26]33].…”

Section: Discussionmentioning

confidence: 99%

Limitation of apoptotic changes and crystal deposition by Tutukon following hyperoxaluria-induced tubular cell injury in rat model

et al. 2015

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This study aimed at evaluating the protective effects of a herbal medication (Tutukon) on the hyperoxaluria induced apoptotic changes and crystal deposition in renal tubular epithelium in rat model. 60 male wistar rats were divided into three different groups (each group n: 20). In Group I severe hyperoxaluria was induced by ethylene glycol (EG) (0.75%) administration for 28 days. In Group II, in addition to hyperoxaluria induction, animals were treated with Tutukon for 28 days. Group III animals constituted the controls without any specific medication and/or intervention. While the presence and degree of crystal deposition in the tubular lumen were examined histopathologically under light microscopy, tubular apoptotic changes were evaluated using immunohistochemical staining for cysteine-aspartic acid protease-3 (Caspase-3) and tumor necrosis factor alpha (TNF-α) positivity on days 14 and 28, respectively. Evaluation of apoptotic changes by Caspase-3 positivity showed that while the majority of animals undergoing EG only showed evident apoptotic changes (n: 9), Tutukon application demonstrated a significant limitation with limited or no apoptosis (n: 7) in these animals. Similar data were noted for TNF alpha expression; while apoptotic changes were evident in 8 (80%) in Group I animals, limited changes were noted in Tutukon Group (n: 2). Regarding crystal deposition despite evident changes in Group I (9 animals), like apoptotic alterations, it was again significantly limited in animals receiving Tutukon (4 animals). Renal tubular crystal deposition and apoptotic changes induced by hyperoxaluria play a role in the pathogenesis of urolithiasis and the limitation of these changes might be instituted by Tutukon as a result of its antioxidant and antiinflammatory effects.

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“…Já nos humanos, embora o aumento na excreção urinária de cálcio também seja importante, a hiperoxalúria é um dos principais fatores de risco de ocorrência de urólitos por oxalato de cálcio (BODAKHE et al, 2013). Pequenos aumentos na concentração urinária de oxalato já exercem efeito litogênico importante.…”

Section: Alterações Metabólicasunclassified

“…O oxalato tem ação citotóxica oxidativa nas células dos túbulos renais e do urotélio de outras regiões do trato urinário que facilitam a adesão de cristais (BODAKHE et al, 2013). Lesões celulares oxidativas em células do trato urinário, podem portanto, induzir à deposição de cristais de oxalato de cálcio.…”

Section: Alterações Metabólicasunclassified

“…Em humanos, as lesões oxidativas podem ocorrer mesmo sob concentrações fisiológicas de oxalato, se as quantidades de antioxidantes endógenos estiverem reduzidas, ou se houver a depleção dos mesmos, o que também pode ocorrer devido à ação do próprio oxalato (THAMILSEVAN et al, 2014). À histologia podem ser identificados depósitos cristalinos nos túbulos renais e necrose difusa do epitélio tubular, o que confirma a lesão renal (BODAKHE et al, 2013).…”

Section: Alterações Metabólicasunclassified

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Etiopatogenia Da Urolitíase Em Cães

Ariza¹

2015

Enciclopédia Biosfera

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RESUMOA urolitíase é comum na clínica de cães e os casos de recidivas são frequentes. Decorre do desenvolvimento de urólitos no trato urinário e pode levar a alterações graves, como obstruções do fluxo urinário. Diversos fatores, sejam hereditários, congênitos ou adquiridos, podem estar envolvidos no desenvolvimento dos cálculos urinários. Os urólitos que mais frequentemente acometem animais da espécie canina são aqueles formados de estruvita, oxalato de cálcio, urato, cistina, fosfato de cálcio, xantina e sílica. No entanto, cálculos urinários com outras composições como de 2,8-diidroxiadenina podem acometer menos frequentemente animais dessa espécie. O conhecimento dos mecanismos que ocasionam a formação e o desenvolvimento dos diferentes tipos de urólito permite a instauração de protocolos de tratamento específicos mais eficientes e que reduzam a possibilidade de ocorrência de recidivas. PALAVRAS-CHAVE: cálculo urinário, canino, patogenia ETIOPATHOGENESIS OF UROLITHIASIS IN DOGS ABSTRACTUrolithiasis are common in dogs and recurrences are frequent. This disease results from the development of uroliths in the urinary tract and may lead to important conditions, such as urinary flow obstructions. Many factors, hereditary, congenital or acquired may be involved in the development of urinary calculi. In dogs, the most common urolith compositions are struvite, calcium oxalate, urate, cystine, calcium phosphate, xanthine and silica. However, urinary calculi with different compositions, such as 2,8-dihydroxyadenine may less frequently affect these animals. Knowledge of the mechanisms involved in the formation and growth of each urolith type allow the establishment of specific treatment protocols that are more efficient and reduce the possibility of recurrences.

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A polyherbal formulation attenuates hyperoxaluria-induced oxidative stress and prevents subsequent deposition of calcium oxalate crystals and renal cell injury in rat kidneys

Cited by 11 publications

References 25 publications

Sodium Thiosulfate Ameliorates Oxidative Stress and Preserves Renal Function in Hyperoxaluric Rats

Sodium Thiosulfate Ameliorates Oxidative Stress and Preserves Renal Function in Hyperoxaluric Rats

Limitation of apoptotic changes and crystal deposition by Tutukon following hyperoxaluria-induced tubular cell injury in rat model

Etiopatogenia Da Urolitíase Em Cães

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