A polymorphism in myostatin influences puberty but not fertility in beef heifers, whereas µ-calpain affects first calf birth weight1

Cushman, Robert A.; Tait, Richard G.; McNeel, Anthony K.; Forbes, EE; Amundson, O. L.; Lents, C. A.; Lindholm‐Perry, A. K.; Perry, George A.; Wood, Jennifer R.; Cupp, Andrea S.; Smith, Ted; Freetly, H. C.; Bennett, G. L.

doi:10.2527/jas.2014-8505

Cited by 17 publications

(13 citation statements)

References 29 publications

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“…the homozygotes and dividing by phenotypic SD. Although genotype effect (8 df) was not significant in this study (P = 0.38; Table 5), birth weight is also shown because it could affect use of F94LaL in mating systems, approached significance (P = 0.06) in heifers that were sibs to these steers (Cushman et al, 2015), and has shown significant increases in other homozygous MSTN mutations (e.g., Casas et al, 2004).…”

Section: Resultscontrasting

confidence: 54%

Enhanced estimates of carcass and meat quality effects for polymorphisms in myostatin and µ-calpain genes1,2,3

Bennett

Tait

Shackelford

et al. 2018

Journal of Animal Science

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The objective of this study was to enhance estimates of additive, dominance, and epistatic effects of marker polymorphisms on beef carcass and quality traits. Myostatin (MSTN) F94L SNP and the µ-calpain (CAPN1) 316 and 4751 SNP haplotype have previously been associated with fat and muscle traits in beef cattle. Multiyear selection in a composite population segregating these polymorphisms increased minor allele (F94L L) and chosen haplotype (CAPN1 CC and GT) frequencies to intermediate levels resulting in more precise estimates of additive and nonadditive genetic effects. During the 3 yr after selection, 176 steers were evaluated for growth, carcass, meat quality, tenderness (n = 103), and meat color traits. The statistical model included year, age of dam, age of the steer, and genotype in a random animal model. The 9 genotypes (3 CAPN1 diplotypes × 3 F94L genotypes) affected marbling score, ribeye area, adjusted fat thickness, vision yield grade (all P < 0.001), slice shear force (P = 0.03), and CIE L* reflectance (P = 0.01). Linear contrasts of the 9 genotypes estimated additive, recessive, and epistatic genetic effects. Significant additive effects of the F94L L allele decreased marbling score, adjusted fat thickness, vision yield grade, and slice shear force; and increased ribeye area and CIE L* reflectance. The homozygous F94L FF and LL genotypes differed by 1.3 to 1.9 phenotypic SD for most carcass traits and by 0.8 to 0.9 SD for slice shear force and CIE L* reflectance but carcass weight differed by only 3 kg (0.1 SD). The L allele was partially recessive to F for ribeye area (P = 0.02) and the heterozygous FL means tended to be closer to the FF genotype than the LL genotype for other carcass traits but differences from additive were not significant. The CAPN1 additive × F94L additive effect on slice shear force was the only significant epistatic estimate. The F94L L allele is prevalent in Limousin but nearly absent in other U.S. purebreds. This allele had about half of the effects on birth weight, muscle, and fat traits reported for severe MSTN mutations in Belgian Blue and Piedmontese breeds. The interaction between MSTN and CAPN1 genotypes may reflect the strong additive effects of MSTN F94L L allele on fat and muscle traits interfering with the phenotypic effect of CAPN1 genotype on meat tenderness.

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Section: Resultscontrasting

confidence: 54%

Enhanced estimates of carcass and meat quality effects for polymorphisms in myostatin and µ-calpain genes1,2,3

Bennett

Tait

Shackelford

et al. 2018

Journal of Animal Science

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“…No significant association was obtained between the c.*194A>G SNP and reproductive traits, including age of puberty by day (AP), kindling interval (KI) and number of services per conception (NSC) as shown in Table 5. Cushman et al (2015) reported an MSTN polymorphism to be significantly associated with a delay in the onset of puberty in cattle while no significant effects were observed in pregnancy rates among the breeds of heifers that were evaluated (Cundiff et al, 2007 andCushman et al, 2015). The cause of this is not clear because the mechanism of action of the MSTN polymorphism is unknown.…”

Section: Resultsmentioning

confidence: 85%

“…However, in many cases, the allele associated with favourable production traits is not always favourable for reproductive traits. For example, some MSTN alleles are associated with longer postpartum anoestrous intervals in cows (Collis et al, 2012), although this same allele had no influence on puberty age in heifers (Cushman et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Correlating single nucleotide polymorphisms in the myostatin gene with performance traits in rabbit

2016

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The Myostatin (MSTN), or Growth and Differentiation Factor 8 (GDF8), gene has been implicated in the double muscling phenomenon, in which a series of mutations render the gene inactive and unable to properly regulate muscle fibre deposition. Single nucleotide polymorphisms (SNPs) in the MSTN gene have been correlated to production traits, making it a candidate target gene to enhance livestock and fowl productivity. This study aimed to assess any association of three SNPs in the rabbit MSTN gene (c.713T>A in exon 2, c.747+34C>T in intron 2, and c.*194A>G in 3'-untranslated region) and their combinations, with carcass, production and reproductive traits. The investigated traits included individual body weight, daily body weight gain, carcass traits and reproductive traits. The 3 SNPs were screened using PCR-restriction fragment length polymorphism (RFLP)-based analysis and the effects of the different SNP genotypes and their combinations were estimated in a rabbit population. Additionally, additive and dominance effects were estimated for significant traits. The results found no significant association between the c.713 T>A SNP and all the examined traits. Allele T at the c.747+34C>T SNP was only significantly associated (P<0.05) with increased body weight at 12 wk of age. However, for the SNP residing in the 3' untranslated region (c.*194A>G), allele G was significantly associated (P<0.05) with increased body weight and high growth rate. Genotype GG at the c.*194A>G SNP also had positive effects on most carcass traits. The estimated additive genetic effect for the c.*194A>G SNP was significant (P<0.05) with most body weight, daily gain and carcass traits. No significant association was obtained between any MSTN SNPs and reproductive traits. In the combinations analysis, regardless of the genotypes of SNPs at c.713T>A and c.747+34C>T, GG at the c.*194A>G SNP correlated with highest values in body weight and daily weight gain. In conclusion, the 'G' allele at the c.*194A>G SNP had positive effects on growth and carcass traits and so could be used as a favourable allele in planning rabbit selection. Further population-wide studies are necessary to test the association of the c.*194A>G SNP with carcass traits. We also recommend evaluation of the potential effects of the c.*194A>G SNP on MSTN gene expression.

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“…In addition, circulating concentrations of both IGF-I (Istasse et al, 1990;ToscanoPagano et al, 2001) and leptin (Daix et al, 2008) are lower in double-muscle cattle compared with non-double-muscle cattle. Recently, the leucine allele in the MSTN F94L SNP, that causes muscle hypertrophy, was linked to a lower proportion of heifers cycling at 11, 12 and 13 months of age (Cushman et al, 2015). Thus, the β-AA treated cattle and the myostatin deficiency cattle are comparable in that both have a reduced fat to muscle ratio, and both have a decrease in serum concentrations of leptin and IGF-I.…”

Section: Discussionmentioning

confidence: 99%

Increased body condition score through increased lean muscle, but not fat deposition, is associated with reduced reproductive response to oestrus induction in beef cows

Guzmán

González-Padilla

Garces-Yepez

et al. 2016

Animal

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Energy reserve, estimated as body condition score (BCS), is the major determinant of the re-initiation of ovarian activity in postpartum cows. Leptin, IGF-I and insulin are positively related to BCS and are putative mediators between BCS and reproductive function. However, when BCS and body composition dissociates, concentrations of these metabolic hormones are altered. We hypothesized that increasing lean muscle tissue, but not fat tissue, would diminish the reproductive response to oestrus induction treatments. Thirty lactating beef cows with BCS of 3.10 ± 1.21 and 75.94 ± 12 days postpartum were divided in two groups. Control cows (n = 15) were supplemented with 10.20 kg of concentrate daily for 60 days. Treated cows (n = 15) were supplemented equally, and received a β-adrenergic receptor agonist (β-AA; 0.15 mg/kg BW) to achieve accretion of lean tissue mass and not fat tissue mass. Twelve days after ending concentrate supplementation/β-AA treatment, cows received a progestin implant to induce oestrus. Cows displaying oestrus were inseminated during the following 60 days, and maintained with a fertile bull for a further 21 days. Cows in both groups gained weight during the supplementation period (Daily weight gain: Control = 0.75 kg v. β-AA = 0.89 kg). Cows treated with β-AA had a larger increase in BCS (i.e. change in BCS: control = 1 point (score 4.13) v. β-AA = 2 points (score 5.06; P < 0.05), as a result of muscle accretion (i.e. change in muscle depth: control 0.21 cm v. β-AA 0.97 cm; P < 0.05) but not adipose tissue (i.e. change in back fat depth; control 0.13 cm v. β-AA −0.06 cm; P < 0.05). The changes in body composition in β-AA cows were associated with a reduction in serum concentrations of IGF-I (25.4%) and leptin (27.9%), without observed changes in insulin. Ovulation and pregnancy to 1st service (P > 0.05) did not differ between groups. However, the number of cows displaying oestrus (control 13/15 v. β-AA 8/15; P < 0.05) and the percentage cycling (control 6/8 v. β-AA 3/10; P = 0.07) after progestin treatment and the pregnancy percentage at the end of the breeding period (control 13/15 v. β-AA 8/15; P < 0.05) were lower in β-AA than control cows. In summary, the increase BCS through muscle tissue accretion, but not through fat tissue accretion, resulted in a lower response to oestrus induction, lower percentage of cycling animals and lower pregnancy percentage after progestin treatment; which was associated with a decrease in serum concentrations of leptin and IGF-I.Keywords: β-AA, energy status, IGF-I, insulin, leptin ImplicationsReproductive function in postpartum beef cows is regulated by energy status and positively associated with serum concentrations of leptin, insulin and IGF-I. β-adrenergic receptor agonist (β-AA) treatment results in muscle accretion, whilst reducing fat deposition. The reduction in body fat, despite the overall BW gain, results in lower leptin and IGF-I concentrations and a suboptimal response to an oestrus induction programme and pregnancy percentage after breeding. He...

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A polymorphism in myostatin influences puberty but not fertility in beef heifers, whereas µ-calpain affects first calf birth weight1

Cited by 17 publications

References 29 publications

Enhanced estimates of carcass and meat quality effects for polymorphisms in myostatin and µ-calpain genes1,2,3

Enhanced estimates of carcass and meat quality effects for polymorphisms in myostatin and µ-calpain genes1,2,3

Correlating single nucleotide polymorphisms in the myostatin gene with performance traits in rabbit

Increased body condition score through increased lean muscle, but not fat deposition, is associated with reduced reproductive response to oestrus induction in beef cows

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