A Polyphenol-Rich Extract From Entada abyssinica Reduces Oxidative Damage in Cryopreserved Ram Semen

Khalil

et al. 2021

Animals

Self Cite

This study evaluated the efficacy of Turraea fischeri leaf extract for maintaining the viability of cryopreserved goat sperm. Ejaculated semen was collected from 5 mature Baladi bucks (50–60 kg, 2–4 years of age) and those samples with mass motility ≥ 70% and sperm concentration ≥ 2.5 × 109/mL were selected, pooled, and divided into 4 aliquots. Each aliquot was diluted in Tris-citric-soybean lecithin extender containing a different concentration of T. fischeri leaf extract (0, 125, 250, or 375 µg/mL). Treated semen samples were cooled to 5 °C, transferred to 0.25-mL French straws, and stored in liquid nitrogen (LN2) at −196 °C. After thawing, membrane integrity was examined by transmission electron microscopy, apoptotic activity by Annexin/propidium iodide staining and flow cytometry, and both enzyme activities and antioxidant capacity by spectroscopic assays. The leaf extract at 375 µg/mL significantly improved semen quality as indicated by enhanced total antioxidant capacity, reduced H2O2 concentration, a greater proportion of structurally intact motile sperm, and concomitant reductions in apoptosis and necrosis. The extract also significantly increased the proportion of sperm with a contiguous plasma membrane and intact acrosome (p < 0.05). Furthermore, LC-MS revealed numerous secondary metabolites in the extract that may contribute to sperm cryopreservation.

Section: Discussionmentioning

confidence: 99%

“…To reduce oxidative stress, exogenous antioxidants are frequently added to cryopreservative solutions to sustain semen quality prior to AI [6]. Several studies have confirmed ROS scavenging capacity of various medicinal plant extracts to improve sperm motility and increase fertility rates [7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and Antiapoptotic Effects of a Turraea fischeri Leaf Extract on Cryopreserved Goat Sperm

Khalil

et al. 2021

Animals

Self Cite

“…Among the compounds identified was dimethyl caffeoyl galloylglucose, with a retention time of 37.86 min and showed a molecular ion peak at (M–H)− m/z 521 with three daughter ions at 331, 271, and 169. The other compounds were cinnamoyl-O-galloylglucose, p-coumaroyl pyrogalloylgalloylglucose, quercetin galloylglucose, catechin gallate, kaempferol syringyl gallate, gentisic acid dipentoside among others ( Sobeh et al, 2020 ). A new peltogynoid, entadanin and monoglyceride, 1′,26′-bis-[(S)-2,3-dihydroxypropyl]hexacosanedioate along with eight known compounds, were isolated from the stem bark of E. abyssinica .…”

Section: Discussionmentioning

confidence: 99%

“…The other compounds isolated included ursolic acid, quercetin-3-O-β-D-glucosyl (1→4)-α-l-rhamnoside, quercetin-3-O-α-l-rhamnoside (quercitrin), 13,14,15,16-tetranor-3-clerodene-12,18-dioic acid, (8S)-kolavic acid 15-methyl ester, methyl gallate ( Melong et al, 2014 ). These chemical compounds have been reported to possess cytotoxicity, antioxidant and antimicrobial activities ( Sobeh et al, 2020 ; Dzoyem et al, 2017 ). Eight compounds isolated from this plant had low cytotoxicity with cytotoxic concentrations ranging between 20 and 80 μg/ml ( Dzoyem et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Sub-Acute Toxicity Effects of Methanolic Stem Bark Extract of Entada abyssinica on Biochemical, Haematological and Histopathological Parameters in Wistar Albino Rats

et al. 2021

Background: Whereas the efficacy of Entada abyssinica (fabaceae) extracts against various ailments has been scientifically validated, its safety has not been established. This study was undertaken to evaluate the toxicity effects of methanolic stem bark extract of E. abyssinica on biochemical, haematological and histological parameters of Wistar albino rats following repeated oral administration.Methods: Wistar albino rats of both sexes were randomized into groups and orally administered daily with determined doses (150, 300 and 600 mg/kg) of E. abyssinica methanolic extract using 1% tween 80 in distilled water as a control for 28 days. On the 29th day, all the animals were sacrificed and dissected to collect blood and selected organs. The serum and whole blood were assayed for biochemical and haematological parameters respectively while selected organs were examined for histopathological lesions. Numerical data was analyzed using graph pad prism and expressed as mean ± standard error of mean. The differences between the treatment and control groups were tested for statistical significance using one-way analysis of variance and/or Student’s t-test.Results: In repeated daily oral doses (150, 300 and 600 mg/kg), the methanolic stem bark extract of E. abyssinica did not cause significant alteration in majority of the biochemical and hematological indices. However, the extract significantly elevated the level of uric acid (all doses), aspartate aminotransferase (300 and 600 mg/kg), low density lipoproteins (150 mg/kg) and mean corpuscular heamoglobin concentration (all doses). On the other hand, the extracts reduced high density lipoproteins (150 and 300 mg/kg), mean corpuscular volume (all doses), haematocrit (150 and 600 mg/kg), mean platelet volume (150 and 600 mg/kg) and procalcitonin (150 mg/kg). In the vital organs, there were no significant lesions observed except at the highest dose (600 mg/kg) where there was mild evidence of lymphocyte infiltration in the liver and focal interstitial nephritis.Conclusion: The methanolic stem bark extract of E. abyssinica is relatively safe in Wistar albino rats when repetitively administered orally in small doses for a prolonged period of time. We recommend more chronic toxicity studies and clinical trials on herbal remedies containing this plant to ensure that its use is free of potential toxicity to humans.

“…This plant has been used as an extender additive for ram semen cryopreservation [ 56 ]. A wide set of concentrations was used (125, 250, 375, and 500 µg/mL).…”

Section: Herbal Pharmacognosy and Its Use In Andrologymentioning

confidence: 99%

Plant Extracts as Alternative Additives for Sperm Preservation

Ros-Santaella

Pintus

2021

Antioxidants

Sperm preservation is a crucial factor for the success of assisted reproductive technology (ART) in humans, livestock, and wildlife. Irrespective of the extender and the storage conditions used, semen handling and preservation negatively affect sperm quality. Moreover, oxidative stress, which often arises during semen storage, significantly reduces sperm function and compromises the sperm fertilizing ability by inducing oxidative damage to proteins, lipids, and nucleic acids. Plant extracts have recently emerged as a cheap and natural source of additives to preserve and enhance sperm function during semen storage. The present work provides an update on the use of these natural compounds as alternative additives for sperm preservation in 13 animal species, including humans. A detailed description of the effects of 45 plant species, belonging to 28 families, on sperm function during semen storage is presented. The plant material and extraction method employed, dosage, possible toxic effects, and antimicrobial properties are provided.