A polysaccharide isolated from Pueraria lobata enhances maturation of murine dendritic cells

Kim, Hyungsook; Shin, Bo Ram; Lee, Hong Kyung; Kim, Yeon Jin; Park, Mi Jung; Kim, Sung Yeon; Lee, Mi Kyeong; Hong, Jin Tae; Kim, Youngsoo; Han, Sang‐Bae

doi:10.1016/j.ijbiomac.2012.09.011

Cited by 27 publications

(15 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been confirmed that TLR-4 could mediated biological activities of many polysaccharides. For instance, polysaccharide from Pueraria lobata (2013), Angelica gigas (2007) and Mori fructus (2013) was found to induce the maturation of DCs derived from normal mice, but not from TLR-4-mutated mice. We found that DCs treated with anti-TLR-4 monoclonal antibody was hyporesponsive to PLP-2 incubation.…”

Section: Discussionmentioning

confidence: 99%

Polysaccharide isolated from seeds of Plantago asiatica L. induces maturation of dendritic cells through MAPK and NF-κB pathway

Jiang

Huang

Nie

et al. 2018

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

species are used as traditional medicine in Asian and Europe. Polysaccharide isolated from the seeds of L. could stimulate maturation transformation of bone-marrow derived dendritic cells (DCs). We found that blocking p38, ERK1/2 and JNK MAPK signal transduction could significantly decreased the PLP-2 induced expression of MHC II, CD86 surface molecules on DCs. Blocking p38 and JNK signal also significantly inhibited the cytokine secretion of TNF-α and IL-12p70 as well, while blocking ERK1/2 signal only decreased the secretion of TNF-α. Meanwhile, DCs in the three MAPK signal-blocking groups showed dramatically attenuated effects on stimulating proliferation of T lymphocytes. Similarly, blocking signal transduction of NF-κB pathway also significantly impaired the phenotypic and functional maturation development of DCs induced by PLP-2. These data suggest that MAPK and NF-κB pathway mediates the PLP-induced maturation on DCs. Especially, among the three MAPK pathways, activation of JNK signal transduction is the most important for DCs development after PLP-2 incubation. And PLP-2 may activate the MAPK and NF-κB pathway by triggering toll-like receptor 4 on DCs.

show abstract

Section: Discussionmentioning

confidence: 99%

Polysaccharide isolated from seeds of Plantago asiatica L. induces maturation of dendritic cells through MAPK and NF-κB pathway

Jiang

Huang

Nie

et al. 2018

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

show abstract

“…As expected, several of the PDPs tested mediated maturation via these pathways. Findings from several studies consistently reported that these polysaccharides increased the phosphorylation of MAPKs, specifically ERK, p38, and JNK and also induced the degradation of IκBα/β and nuclear translocation of p65 and p50 (Kim et al 2013;Park et al 2014;Shin et al 2013). On further analysis, some of these downstream molecules play a bigger role in DC maturation.…”

Section: Tlr Downstream Signaling Pathwaysmentioning

confidence: 83%

“…Indeed, of the eleven PDPs tested for mechanism in this review, Li et al (2010) ten reported involvement of TLR4 signalling pathways in phenotypic and functional maturation of DCs. Most tested PDPs efficiently increased the maturation of TLR4 +/+ DCs but C3H/HeJ mice with mutated TLR4 were hyporesponsive to angelan (a pure polysaccharide extract of Angelica gigas Nakai), Mori fructus polysaccharide, Pueraria lobata polysaccharide, Platycodon grandiflorum polysaccharide and LPS (Kim et al 2007(Kim et al , 2013Park et al 2014;Shin et al 2013). Nevertheless, introduction of TLR3 ligand poly(I:C) still enhanced DC maturation and function (Kim et al 2013) indicating that although TLR4 appears to be the main pathway, other complementary pathways may be involved.…”

Section: Membrane Receptorsmentioning

confidence: 99%

“…Most tested PDPs efficiently increased the maturation of TLR4 +/+ DCs but C3H/HeJ mice with mutated TLR4 were hyporesponsive to angelan (a pure polysaccharide extract of Angelica gigas Nakai), Mori fructus polysaccharide, Pueraria lobata polysaccharide, Platycodon grandiflorum polysaccharide and LPS (Kim et al 2007(Kim et al , 2013Park et al 2014;Shin et al 2013). Nevertheless, introduction of TLR3 ligand poly(I:C) still enhanced DC maturation and function (Kim et al 2013) indicating that although TLR4 appears to be the main pathway, other complementary pathways may be involved. In another study to investigate the relative importance of the various TLRs, Huang and colleagues reported that P. asiatica polysaccharides significantly upregulated TLR2 and TLR4 mRNA expression and to a lesser extent TLR7 and TLR9.…”

Section: Membrane Receptorsmentioning

confidence: 99%

See 1 more Smart Citation

Plant-derived polysaccharides activate dendritic cell-based anti-cancer immunity

et al. 2018

View full text Add to dashboard Cite

Today, cancers pose a major public health burden. Although a myriad of cancer treatments are available, only a few have achieved clinical efficacy. This is partly attributed to cancers capability to evade host immunity by converting dendritic cells (DCs) from potent stimulators to negative modulators of immunity. Dendritic cell-based immunotherapy attempts to resolve this problem by manipulating the functional characteristics of DCs. Plant-derived polysaccharides (PDPs) can stimulate the maturation of DCs conferring on them the capacity to present internalised tumorigenic antigens to naïve T cells and subsequently priming T cells to eliminate tumours. PDPs have been used as immune modulators and later as anti-cancer agents by Traditional Chinese Medicine practitioners for centuries. They are abundant in nature and form a large group of heterogeneous though structurally related macromolecules that exhibit diverse immunological properties. They can induce antigen pulsed DCs to acquire functional characteristics in vitro which can subsequently be re-introduced into cancer patients. They can also be used as adjuvants in DC-based vaccines or independently for their intrinsic anti-tumour activities. Clinically, some in vitro generated DCs have been shown to be both safe and immunogenic although their clinical application is limited in part by unsatisfactory functional maturation as well as impaired migration to draining lymph nodes where T cells reside. We review the relative potencies of individual PDPs to induce both phenotypic and functional maturation in DCs, their relative abilities to activate anti-cancer immunity, the possible mechanisms by which they act and also the challenges surrounding their clinical application.

show abstract

“…Polysaccharides are a kind of polymers formed by the connection of aldose or ketose through glycoside bonds and are found mainly in plants, animals, and microorganisms. Studies have shown that polysaccharides have a wide range of pharmacologic activities, such as antitumor, immune regulation, antiviral, antiaging, hypoglycemic, and blood lipid activities, especially in antitumor immunity with minimal adverse reactions 175‐178 . For example, Portulaca oleracea L .…”

Section: Molecular Mechanisms On Natural Products In Tumor Immunotherapymentioning

confidence: 99%

Natural products and their derivatives: Promising modulators of tumor immunotherapy

Deng

et al. 2020

Journal of Leukocyte Biology

145

View full text Add to dashboard Cite

A wealth of evidence supports the role of tumor immunotherapy as a vital therapeutic option in cancer. In recent decades, accumulated studies have revealed the anticancer activities of natural products and their derivatives. Increasing interest has been driven toward finding novel potential modulators of tumor immunotherapy from natural products, a hot research topic worldwide. These works of research mainly focused on natural products, including polyphenols (e.g., curcumin, resveratrol), cardiotonic steroids (e.g., bufalin and digoxin), terpenoids (e.g., paclitaxel and artemisinins), and polysaccharide extracts (e.g., lentinan). Compelling data highlight that natural products have a promising future in tumor immunotherapy. Considering the importance and significance of this topic, we initially discussed the integrated research progress of natural products and their derivatives, including target T cells, macrophages, B cells, NKs, regulatory T cells, myeloid-derived suppressor cells, inflammatory cytokines and chemokines, immunogenic cell death, and immune checkpoints. Furthermore, these natural compounds inactivate several key pathways, including NF-B, PI3K/Akt, MAPK, and JAK/STAT pathways. Here, we performed a deep generalization, analysis, and summarization of the previous achievements, recent progress, and the bottlenecks in the development of natural products as tumor immunotherapy. We expect this review to provide some insight for guiding future research.

show abstract

A polysaccharide isolated from Pueraria lobata enhances maturation of murine dendritic cells

Cited by 27 publications

References 38 publications

Polysaccharide isolated from seeds of Plantago asiatica L. induces maturation of dendritic cells through MAPK and NF-κB pathway

Polysaccharide isolated from seeds of Plantago asiatica L. induces maturation of dendritic cells through MAPK and NF-κB pathway

Plant-derived polysaccharides activate dendritic cell-based anti-cancer immunity

Natural products and their derivatives: Promising modulators of tumor immunotherapy

Contact Info

Product

Resources

About