A porcine acellular dermal matrix induces human fibroblasts to secrete hyaluronic acid by activating JAK2/STAT3 signalling

Liu, Chao; Sun, Jiao

doi:10.1039/c9ra03736e

Cited by 7 publications

(9 citation statements)

References 41 publications

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“…Additionally, skin barrier function and hydration are affected by Src/AKT/NF-κB and MAPK signaling [34,35]. The proximal promoter of HAS2 has transcription factor-binding sites for the JAK2/STAT3 pathway [36]. Rauhala et al found that the active purinergic P2Y2 receptor largely promotes HAS2 expression and several signaling pathways are associated with HAS2 regulation, such as Ca 2+ /calmodulin-dependent protein kinase II (CaMKII), calcium-activated PKC, calcium response element-binding protein (CREB), and MAP kinase cascades [28].…”

Section: Discussionmentioning

confidence: 99%

Kahweol Exerts Skin Moisturizing Activities by Upregulating STAT1 Activity

Chen

Hossain

Kim

et al. 2021

IJMS

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Kahweol is a diterpene present in coffee. Until now, several studies have shown that kahweol has anti-inflammatory and anti-angiogenic functions. Due to the limited research available about skin protection, this study aims to discern the potential abilities of kahweol and the possible regulation targets. First, the cytotoxicity of kahweol was checked by 3-4-5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide assay, while 2,20-azino-bis (3ethylbenzothiazoline-6-sulphonic acid) diammonium salt and 1-diphenyl-2-picryl-hydrazyl were used to examine the radical scavenging ability. Polymerase chain reaction analysis was performed to explore the proper time points and doses affecting skin hydration and barrier-related genes. Luciferase assay and Western blotting were used to explore the possible transcription factors. Finally, fludarabine (a STAT1 inhibitor) was chosen to discern the relationship between skin-moisturizing factors and STAT1. We found that HaCaT cells experienced no toxicity from kahweol, and kahweol displayed moderate radical scavenging ability. Moreover, kahweol increased the outcome of HAS1, HAS2, occludin, and TGM-1 from six hours in a dose-dependent manner as well as the activation of STAT1 from six hours. Additionally, kahweol recovered the suppression of HAS2, STAT1-mediated luciferase activity, and HA secretion, which was all downregulated by fludarabine. In this study, we demonstrated that kahweol promotes skin-moisturizing activities by upregulating STAT1.

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Section: Discussionmentioning

confidence: 99%

Kahweol Exerts Skin Moisturizing Activities by Upregulating STAT1 Activity

Chen

Hossain

Kim

et al. 2021

IJMS

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“…ECM has been known as an essential member of myocardial healing following ischemic injury and a crucial mediator for cell regeneration and endogenous tissue reconstruction [24][25][26]. Natural acellular tissue 3D cell culture systems have attracted increasing interest in tissue engineering due to better simulating the microenvironment where in vivo cells grow [27][28][29][30][31][32]. This study investigated the growth, proliferation, and angiogenesis signaling pathway in cardiomyocytes derived from isoproterenol-induced MI and healthy mice cultured on the acellular aortic matrix (3D system) and compared them with healthy and injured cells cultured in a 2D system.…”

Section: Discussionmentioning

confidence: 99%

Cardiac aorta-derived extracellular matrix scaffold enhances critical mediators of angiogenesis in isoproterenol-induced myocardial infarction mice

Hosseinabadi

Abdolmaleki

Beheshtiha

2021

J Mater Sci: Mater Med

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An incapability to improve lost cardiac muscle caused by acute ischemic injury remains the most important deficiency of current treatments to prevent heart failure. We investigated whether cardiomyocytes culturing on cardiac aorta-derived extracellular matrix scaffold has advantageous effects on cardiomyocytes survival and angiogenesis biomarkers’ expression. Ten male NMRI mice were randomly divided into two groups: (1) control (healthy mice) and (2) myocardial infarction (MI)-induced model group (Isoproterenol/subcutaneously injection/single dose of 85 mg/kg). Two days after isoproterenol injection, all animals were sacrificed to isolate cardiomyocytes from myocardium tissues. The fresh thoracic aorta was obtained from male NMRI mice and decellularized using 4% sodium deoxycholate and 2000 kU DNase-I treatments. Control and MI-derived cardiomyocytes were seeded on decellularized cardiac aorta (DCA) considered three-dimensional (3D) cultures. To compare, the isolated cardiomyocytes from control and MI groups were also cultured as a two-dimensional (2D) culture system for 14 days. The cell viability was examined by MTT assay. The expression levels of Hif-1α and VEGF genes and VEGFR1 protein were tested by real-time PCR and western blotting, respectively. Moreover, the amount of VEGF protein was evaluated in the conditional media of the 2D and 3D systems. The oxidative stress was assessed via MDA assay. Hif-1α and VEGF genes were downregulated in MI groups compared to controls. However, the resulting data showed that decellularized cardiac aorta matrices positively affect the expression of Hif-1α and VEGF genes. The expression level of VEGFR1 protein was significantly (p ≤ 0.01) upregulated in both MI and healthy cell groups cultured on decellularized cardiac aorta matrices as a 3D system compared to the MI cell group cultured in the 2D systems. Furthermore, MDA concentration significantly decreased in 3D-cultured cells (MI and healthy cell groups) rather than the 2D-cultured MI group (p ≤ 0.015). The findings suggest that cardiac aorta-derived extracellular scaffold by preserving VEGF, improving the cell viability, and stimulating angiogenesis via upregulating Hif-1α, VEGF, and VEGFR1 in cardiomyocytes could be considered as a potential approach along with another therapeutic method to reduce the complications of myocardial infarction and control the progressive pathological conditions related to MI.

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“…Hst1 was dissolved in 1 ml 0.9% NaCl and supplemented with Hst1 formulations at final concentrations of 1, 10, and 50 μM. The ADM (Jiangsu Unitrump Biomedical Technology Co., Ltd., Nanjing, China) is an enzymatically digested product in a form of ultrafine powder with an average particle size at 33.4 μm ( Liu and Sun, 2020 ). Before application, ADM powder was mixed with different concentrations of Hst1 at a volume ratio of 1:1 (v/v) through three-way pipes for 1 h, forming into pastes.…”

Section: Methodsmentioning

confidence: 99%

Human Salivary Histatin-1 Is More Efficacious in Promoting Acute Skin Wound Healing Than Acellular Dermal Matrix Paste

Lei

Cheng

Lin

et al. 2020

Front. Bioeng. Biotechnol.

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A porcine acellular dermal matrix induces human fibroblasts to secrete hyaluronic acid by activating JAK2/STAT3 signalling

Abstract: By activating the JAK2/STAT3 pathway, 3D-GF-PADM induces the production of hyaluronic acid in human fibroblasts.

Cited by 7 publications

References 41 publications

Kahweol Exerts Skin Moisturizing Activities by Upregulating STAT1 Activity

Kahweol Exerts Skin Moisturizing Activities by Upregulating STAT1 Activity

Cardiac aorta-derived extracellular matrix scaffold enhances critical mediators of angiogenesis in isoproterenol-induced myocardial infarction mice

Human Salivary Histatin-1 Is More Efficacious in Promoting Acute Skin Wound Healing Than Acellular Dermal Matrix Paste

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