A portable and affordable aligner for the assembly of microfluidic devices

Guglielmotti, Victoria; Saffioti, Nicolás A.; Tohmé, Ana Laura; Gambarotta, Martín; Corthey, Gastón; Pallarola, Diego

doi:10.1016/j.ohx.2022.e00348

Cited by 3 publications

(1 citation statement)

References 19 publications

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“…4. Align and bond the two parts together using either a mask aligner [19] or a custom tabletop aligner [20], [21]. 5.…”

Section: Preparation Of the Microfluidic Chipsmentioning

confidence: 99%

Magnetic Microtweezers for High-Throughput Bioseparation in Sub-Nanoliter Droplets

Dumas,

Alexandre,

Richerd

et al. 2024

Preprint

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Multiomics studies at single-cell level require small volume manipulation, high throughput analysis and multiplexed detection, characteristics that droplet microfluidics can tackle. However, the initial step of molecules bioseparation remains challenging. Here, we describe a unique magnetic device to trap and extract magnetic particles in sub-nanoliter droplets, for compartmentalisation of detection steps. Relying on electrodeposition of NiFe structures and microfluidic manipulation, this technology has allowed the purification of genetic material at single-cell scale and was able to reach an extraction rate of 72% for a sample of purified oligonucleotides.

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“…4. Align and bond the two parts together using either a mask aligner [19] or a custom tabletop aligner [20], [21]. 5.…”

Section: Preparation Of the Microfluidic Chipsmentioning

confidence: 99%

Magnetic Microtweezers for High-Throughput Bioseparation in Sub-Nanoliter Droplets

Dumas,

Alexandre,

Richerd

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

Magnetic Microtweezers for High-Throughput Bioseparation in Sub-Nanoliter Droplets

Dumas,

Alexandre,

Richerd

et al. 2024

Methods in Molecular Biology

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Microfluidics based bioimaging with cost-efficient fabrication of multi-level micrometer-sized trenches

et al. 2023

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Microfluidic devices, through their vast applicability as tools for miniaturized experimental setups, have become indispensable for cutting edge research and diagnostics. However, the high operational cost and the requirement of sophisticated equipment and clean room facility for the fabrication of these devices make their use unfeasible for many research laboratories in resource limited settings. Therefore, with the aim of increasing accessibility, in this article, we report a novel, cost-effective micro-fabrication technique for fabricating multi-layer microfluidic devices using only common wet-lab facilities, thereby significantly lowering the cost. Our proposed process-flow-design eliminates the need for a mastermold, does not require any sophisticated lithography tools, and can be executed successfully outside a clean room. In this work, we also optimized the critical steps (such as spin coating and wet etching) of our fabrication process and validated the process flow and the device by trapping and imaging Caenorhabditis elegans. The fabricated devices are effective in conducting lifetime assays and flushing out larvae, which are, in general, manually picked from Petri dishes or separated using sieves. Our technique is not only cost effective but also scalable, as it can be used to fabricate devices with multiple layers of confinements ranging from 0.6 to more than 50 μm, thus enabling the study of unicellular and multicellular organisms. This technique, therefore, has the potential to be adopted widely by many research laboratories for a variety of applications.

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A portable and affordable aligner for the assembly of microfluidic devices

Cited by 3 publications

References 19 publications

Magnetic Microtweezers for High-Throughput Bioseparation in Sub-Nanoliter Droplets

Magnetic Microtweezers for High-Throughput Bioseparation in Sub-Nanoliter Droplets

Magnetic Microtweezers for High-Throughput Bioseparation in Sub-Nanoliter Droplets

Microfluidics based bioimaging with cost-efficient fabrication of multi-level micrometer-sized trenches

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