2021
DOI: 10.1101/2021.11.22.469235
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A portable and high-integrated 3D microfluidic chip for bacterial quantification and antibiotic susceptibility testing

Abstract: On-site single-cell antibiotic susceptibility testing (sc-AST) provides unprecedented technical potential to improve the treatment of bacterial infections and study heterogeneous resistance to antibiotics. Herein, we developed a portable and high-integrated 3D polydimethylsiloxane (PDMS) chip to perform fast and on-site bacteria quantification and sc-AST. The 3D arrangement of the chambers significantly improved the integration of reaction units (∼10000/cm2) and widened the dynamic range to 5 orders of magnitu… Show more

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