A portable and integrated nucleic acid amplification microfluidic chip for identifying bacteria

Abstract

In this work, we developed a portable integrated microchip of loop-mediated isothermal nucleic acid amplification (LAMP). This chip, with sample-to-answer capability, could perform rapid DNA release, exponential signal amplification and naked-eye result read-out in single or multiplex format. We call it iμLAMP, namely integrated micro-LAMP, which was successfully used for point-of-care identification of bacteria.

Smartphone-based, sensitive µPAD detection of urinary tract infection and gonorrhea

“…There are a few methods that do report low detection limits within 100 CFU/mL (lines 3, 6, 12 and 13; Safavieh et al, 2012;Wang et al, 2012;Fang et al, 2012;Safavieh et al, 2014). Most of these "low detection limit" methods utilized loop-mediated isothermal amplification (LAMP), which amplified DNA targets to increase the concentration for their assays (lines 3, 12 and 13; Safavieh et al, 2012;Fang et al, 2012;Safavieh et al, 2014), as well as the use of relatively clean sample matrix (e.g. buffer; lines 12 and 13; Fang et al, 2012;Safavieh et al, 2014).…”

“…Most of these "low detection limit" methods utilized loop-mediated isothermal amplification (LAMP), which amplified DNA targets to increase the concentration for their assays (lines 3, 12 and 13; Safavieh et al, 2012;Fang et al, 2012;Safavieh et al, 2014), as well as the use of relatively clean sample matrix (e.g. buffer; lines 12 and 13; Fang et al, 2012;Safavieh et al, 2014). In contrast to our near-real-time assay, none of the referenced methods are close to real-time and may take hours for results.…”

Emerging technologies for monitoring drug-resistant tuberculosis at the point-of-care

“…Another partially integrated portable microfluidic chip has been developed to enable sample-in to TB detection-out [65]. In this device, the microfluidic chip contains separate chambers for DNA extraction and amplification/detection.…”

High-throughput sample-to-answer detection of DNA/RNA in crude samples within functionalized micro-pipette tips

“…In fact, the prevalence of infectious diseases in developing countries is much higher than that in developed world (Lee et al 2010;Peeling and Mabey 2010). Therefore, it is urgent to develop a sample-to-answer, easy-to-operate, rapid, low-cost, and robust NAT with minimal instrumentation (Bissonnette and Bergeron 2010;Dou et al 2014;Fang et al 2012;Kurosaki et al 2007;Leng et al 2010;Liu et al 2013;Urdea et al 2006;Zhu et al 2012). …”

“…Nucleic acid (deoxyribonucleic acid, DNA and ribonucleic acid, RNA) analysis is emerging as one of the most effective methods in biomedical applications Fang et al 2012;Fang et al 2010a;Fang et al 2010b;Liu et al 2011;Sun et al 2014;Wu et al 2015;Xie et al 2015;Zhang et al 2014a;Zhang et al 2014b), especially for diagnosis of infectious diseases.…”