A Portable Device for LAMP Based Detection of SARS-CoV-2

Sreejith, Kamalalayam Rajan; Umer, Muhammad; Dirr, Larissa; Bailly, Benjamin; Guillon, Patrice; Itzstein, Mark von; Soda, Narshone; Kasetsirikul, Surasak; Shiddiky, Muhammad J. A.; Nguyen, Nam‐Trung

doi:10.3390/mi12101151

Cited by 14 publications

(20 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Isothermal amplification techniques range in terms of total quantity of primers and enzymes employed, the temperature at which the amplification takes place, and the sorts of templates used. Table 2 [75][76][77][78][79][80][81][82][83][84][85][86][87] summarizes the several isothermal amplification platforms established for detecting SARS-CoV-2 in both laboratory and clinical samples.…”

Section: Isothermal Amplificationmentioning

confidence: 99%

See 1 more Smart Citation

Diagnostic assay and technology advancement for detecting SARS-CoV-2 infections causing the COVID-19 pandemic

Dhar

2022

Anal Bioanal Chem

View full text Add to dashboard Cite

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-caused COVID-19 pandemic has transmitted to humans in practically all parts of the world, producing socio-economic turmoil. There is an urgent need for precise, fast, and affordable diagnostic testing to be widely available for detecting SARS-CoV-2 and its mutations in various phases of the disease. Early diagnosis with great precision has been achieved using real-time polymerase chain reaction (RT-PCR) and similar other molecular methods, but theseapproaches are costly and involve rigorous processes that are not easily obtainable. Conversely, immunoassays that detect a small number of antibodies have been employed for quick, low-cost tests, but their efficiency in diagnosing infected people has been restricted. The use of biosensors in the detection of SARS-CoV-2 is vital for the COVID-19 pandemic's control. This review gives an overview of COVID-19 diagnostic approaches that are currently being developed as well as nanomaterial-based biosensor technologies, to aid future technological advancement and innovation. These approaches can be integrated into point-of-care (POC) devices to quickly identify a large number of infected patients and asymptomatic carriers. The ongoing research endeavors and developments in complementary technologies will play a significant role in curbing the spread of the COVID-19 pandemic and fill the knowledge gaps in current diagnostic accuracy and capacity.

show abstract

Section: Isothermal Amplificationmentioning

confidence: 99%

“…Summary of the several isothermal amplification platforms established for SARS-CoV-2 diagnostics in both laboratory and clinical samples [Refs [75][76][77][78][79][80][81][82][83][84][85][86][87]. …”

mentioning

confidence: 99%

Diagnostic assay and technology advancement for detecting SARS-CoV-2 infections causing the COVID-19 pandemic

Dhar

2022

Anal Bioanal Chem

View full text Add to dashboard Cite

show abstract

“…The results showed that 35 cycles of PCR were conducted within 10 min, and three types of human coronavirus (OC43, 229E, and NL63) could be simultaneously detected by distinct fluorescence signals. The fluorescence-based detection showed the potential of our thermal cycler to be utilized for quantitative analysis by coupling with a real-time monitoring setup [ 30 ]. These practical applications can pave the way for their application in various biomedical applications.…”

Section: Discussionmentioning

confidence: 99%

Conductive Silver/Carbon Fiber Films for Rapid Detection of Human Coronavirus

et al. 2022

View full text Add to dashboard Cite

Polymerase chain reaction has gained attention since the outbreak of novel coronavirus in 2019. Due to its high specificity and capability for early detection, it is considered a standard method for the diagnosis of infectious diseases. However, the conventional thermocyclers used for nucleic acid amplification are not suitable for point-of-care testing applications, as they require expensive instruments, high-power consumption, and a long turnaround time. To suppress the widespread of the pandemic, there is an urgent need for the development of a rapid, inexpensive, and portable thermal cycler. Therefore, in this paper, we present a conductive silver/carbon fiber film-based thermal cycler with low power consumption (<5 W), efficient heating (~4.5 °C/s), low cost (<USD 200), and handheld size (11.5 × 7.1 × 7.5 mm). The conductive film, which was used as a heating source of the thermal cycler, was fabricated by the electrochemical deposition method. The successful coating of Ag was characterized by a scanning electron microscope and confirmed by energy-dispersive X-ray spectroscopy. The film showed excellent electrical/thermal conductivity and durability. Using our thermal cycler, 35 cycles of amplification were accomplished within 10 min. We also successfully demonstrated the multiplexed detection of various human coronaviruses (e.g., OC43, 229E, and NL63) using our thermal cycler.

show abstract

“…Additionally, in 2021, a research group from Griffith University fabricated a portable LAMP device targeting SARS-CoV-2 based on fluorescence emission ( Figure 6 C) [ 107 ]. According to Sreejith et al, the system (costing USD 100) consists of an “aluminum block embedded with a cartridge heater,” a “single-board computer and camera,” and an LED.…”

Section: A Look Into the Point-of-care Lamp Market: Research And Comm...mentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Progression of LAMP as a Result of the COVID-19 Pandemic: Is PCR Finally Rivaled?

Mannier

Yoon

2022

Biosensors

View full text Add to dashboard Cite

Reflecting on the past three years and the coronavirus disease 19 (COVID-19) pandemic, varying global tactics offer insights into the most effective public-health responses. In the US, specifically, rapid and widespread testing was quickly prioritized to lower restrictions sooner. Essentially, only two types of COVID-19 diagnostic tests were publicly employed during the peak pandemic: the rapid antigen test and reverse transcription polymerase chain reaction (RT-PCR). However, neither test ideally suited the situation, as rapid antigen tests are far too inaccurate, and RT-PCR tests require skilled personnel and sophisticated equipment, leading to long wait times. Loop-mediated isothermal amplification (LAMP) is another exceptionally accurate nucleic acid amplification test (NAAT) that offers far quicker time to results. However, RT-LAMP COVID-19 tests have not been embraced as extensively as rapid antigen tests or RT-PCR. This review will investigate the performance of current RT-LAMP-based COVID-19 tests and summarize the reasons behind the hesitancy to embrace RT-LAMP instead of RT-PCR. We will also look at other LAMP platforms to explore possible improvements in the accuracy and portability of LAMP, which could be applied to COVID-19 diagnostics and future public-health outbreaks.

show abstract

A Portable Device for LAMP Based Detection of SARS-CoV-2

Cited by 14 publications

References 25 publications

Diagnostic assay and technology advancement for detecting SARS-CoV-2 infections causing the COVID-19 pandemic

Diagnostic assay and technology advancement for detecting SARS-CoV-2 infections causing the COVID-19 pandemic

Conductive Silver/Carbon Fiber Films for Rapid Detection of Human Coronavirus

Progression of LAMP as a Result of the COVID-19 Pandemic: Is PCR Finally Rivaled?

Contact Info

Product

Resources

About