A Portable Multi-Channel Turbidity System for Rapid Detection of Pathogens by Loop-Mediated Isothermal Amplification

Chen, Zhu; Yang, Tao; Yang, Haowen; Li, Taotao; Nie, Libo; Mou, Xianbo; Deng, Yan; He, Nongyue; Li, Zhiyang; Wang, Lijun; Li, Song

doi:10.1166/jbn.2018.2524

Cited by 32 publications

(16 citation statements)

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“…At present, constant temperature amplification techniques applied to foodborne pathogenic microorganisms mainly include loop-mediated isothermal amplification (LAMP), recombinant enzyme-mediated nucleic acid amplification (RAA), RPA, and nucleic acid sequence-dependent amplification (NASBA) ( Chen et al, 2018 ; Khan et al, 2019 ; Safenkova et al, 2020 ; He et al, 2021a ; Hoang et al, 2021 ; Tian et al, 2022 ).…”

Section: Molecular Biology Detection Technologymentioning

confidence: 99%

Research progress on detection techniques for point-of-care testing of foodborne pathogens

Zhao

Huang

et al. 2022

Front. Bioeng. Biotechnol.

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The global burden of foodborne disease is enormous and foodborne pathogens are the leading cause of human illnesses. The detection of foodborne pathogenic bacteria has become a research hotspot in recent years. Rapid detection methods based on immunoassay, molecular biology, microfluidic chip, metabolism, biosensor, and mass spectrometry have developed rapidly and become the main methods for the detection of foodborne pathogens. This study reviewed a variety of rapid detection methods in recent years. The research advances are introduced based on the above technical methods for the rapid detection of foodborne pathogenic bacteria. The study also discusses the limitations of existing methods and their advantages and future development direction, to form an overall understanding of the detection methods, and for point-of-care testing (POCT) applications to accurately and rapidly diagnose and control diseases.

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Section: Molecular Biology Detection Technologymentioning

confidence: 99%

Research progress on detection techniques for point-of-care testing of foodborne pathogens

Zhao

Huang

et al. 2022

Front. Bioeng. Biotechnol.

Self Cite

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“…The targets of biosensors also include the pathogens that cause diseases in humans, such as cancerous cells, harmful bacteria, and viruses [ 127 , 128 , 129 , 130 , 131 ]. The main method of detecting cells and bacteria was achieved by the interaction between cell membranes and compounds that have a strong affinity for cells and bacteria [ 132 , 133 ].…”

Section: Overview Of Lc Droplet-based Biosensors For Poc Diagnosis Of...mentioning

confidence: 99%

Liquid Crystal Droplet-Based Biosensors: Promising for Point-of-Care Testing

Xie

et al. 2022

Biosensors

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The development of biosensing platforms has been impressively accelerated by advancements in liquid crystal (LC) technology. High response rate, easy operation, and good stability of the LC droplet-based biosensors are all benefits of the long-range order of LC molecules. Bioprobes emerged when LC droplets were combined with biotechnology, and these bioprobes are used extensively for disease diagnosis, food safety, and environmental monitoring. The LC droplet biosensors have high sensitivity and excellent selectivity, making them an attractive tool for the label-free, economical, and real-time detection of different targets. Portable devices work well as the accessory kits for LC droplet-based biosensors to make them easier to use by anyone for on-site monitoring of targets. Herein, we offer a review of the latest developments in the design of LC droplet-based biosensors for qualitative target monitoring and quantitative target analysis.

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“…These devices provide a wide range of qualitative or quantitative measurements for analysis [ 32 , 33 ]. Creating a chip-based [ 34 ], miniature [ 35 ], transportable [ 36 ], and self-contained device [ 37 ] that analyzes various analytes in intricate samples is one of the objectives of POCT [ 38 , 39 , 40 ]. POCT represents a paradigm change from traditional diagnostic tests in the clinical laboratory environment to near-patient settings, enabling doctors to make more informed decisions about diagnosis and treatment by giving them access to rapid diagnostic information.…”

Section: Introductionmentioning

confidence: 99%

Application of Janus Particles in Point-of-Care Testing

et al. 2022

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Janus particles (JPs), named after the two-faced Roman god, are asymmetric particles with different chemical properties or polarities. JPs have been widely used in the biomedical field in recent years, including as drug carriers for targeted controlled drug release and as biosensors for biological imaging and biomarker detection, which is crucial in the early detection and treatment of diseases. In this review, we highlight the most recent advancements made with regard to Janus particles in point-of-care testing (POCT). Firstly, we introduce several commonly used methods for preparing Janus particles. Secondly, we present biomarker detection using JPs based on various detection methods to achieve the goal of POCT. Finally, we discuss the challenges and opportunities for developing Janus particles in POCT. This review will facilitate the development of POCT biosensing devices based on the unique properties of Janus particles.

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A Portable Multi-Channel Turbidity System for Rapid Detection of Pathogens by Loop-Mediated Isothermal Amplification

Cited by 32 publications

References 0 publications

Research progress on detection techniques for point-of-care testing of foodborne pathogens

Research progress on detection techniques for point-of-care testing of foodborne pathogens

Liquid Crystal Droplet-Based Biosensors: Promising for Point-of-Care Testing

Application of Janus Particles in Point-of-Care Testing

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