A Portable Surface Plasmon Resonance Biosensor for Rapid Detection of Salmonella typhimurium

Nguyen, Hoang Hiep; Yi, So Yeon; Abdela, Woubit; Kim, Moonil

doi:10.5757/asct.2016.25.3.61

Cited by 20 publications

(13 citation statements)

References 16 publications

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“…Figure 1 B shows a schematic illustration of the portable SPR system based on the modulation of laser light by a rotating mirror. Conventional SPR systems adopt the prism-based Kretschmann configuration, in which a laser is used as the light source and the SPR angle is measured [ 24 ]. Oscillation mirror-based instruments, in which a mirror adjusts the incident angle at which surface plasmons are excited, have also been widely used [ 24 ].…”

Section: Resultsmentioning

confidence: 99%

On-Site Detection of Aflatoxin B1 in Grains by a Palm-Sized Surface Plasmon Resonance Sensor

Moon

Byun

Kim

et al. 2018

Sensors

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Aflatoxins (AFs) are highly toxic compounds that can cause both acute and chronic toxicity in humans. Aflatoxin B1 (AFB1) is considered the most toxic of AFs. Therefore, the rapid and on-site detection of AFB1 is critical for food safety management. Here, we report the on-site detection of AFB1 in grains by a portable surface plasmon resonance (SPR) sensor. For the detection of AFB1, the surface of an SPR Au chip was sequentially modified by cysteine-protein G, AFB1 antibody, and bovine serum albumin (BSA). Then, the sample solution and AFB1-BSA conjugate were flowed onto the Au chip in serial order. In the absence of AFB1, the SPR response greatly increased due to the binding of AFB1-BSA on the Au chip. In the presence of AFB1, the SPR response showed little change because the small AFB1 molecule binds on the Au chip instead of the large AFB1-BSA molecule. By using this portable SPR-based competitive immunoassay, the sensor showed low limits of detection (2.51 ppb) and quantification (16.32 ppb). Furthermore, we successfully detected AFB1 in rice, peanut, and almond samples, which suggests that the proposed sensing method can potentially be applied to the on-site monitoring of mycotoxins in food.

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Section: Resultsmentioning

confidence: 99%

On-Site Detection of Aflatoxin B1 in Grains by a Palm-Sized Surface Plasmon Resonance Sensor

Moon

Byun

Kim

et al. 2018

Sensors

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“…In the past decades, tremendous efforts have been made to develop various biosensors for detection of foodborne pathogenic bacteria, such as electrochemical biosensors 5-7, quartz crystal microbalance (QCM) biosensors 8, 9, surface plasmon resonance (SPR) biosensors 10, 11, optical biosensors 12, 13, etc. These biosensors have shown the advantages of high sensitivity, good specificity, short detection time, simple operation and low cost.…”

Section: Introductionmentioning

confidence: 99%

An enzyme-free biosensor for sensitive detection of Salmonella using curcumin as signal reporter and click chemistry for signal amplification

Huang

Zhang

et al. 2018

Theranostics

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In this study, an enzyme-free biosensor was developed for sensitive and specific detection of Salmonella typhimurium (S. typhimurium) using curcumin (CUR) as signal reporter and 1,2,4,5-tetrazine (Tz)-trans-cyclooctene (TCO) click chemistry for signal amplification.Methods: Nanoparticles composed of CUR and bovine serum albumin (BSA) were formulated and reacted with Tz and TCO to form Tz-TCO-CUR conjugates through Tz-TCO click chemistry. Then, the Tz-TCO-CUR conjugates were functionalized with polyclonal antibodies (pAbs) against S. typhimurium to form CUR-TCO-Tz-pAb conjugates. Magnetic nanoparticles (MNPs) conjugated with monoclonal antibodies (mAbs) against S. typhimurium through streptavidin-biotin binding were used to specifically and efficiently separate S. typhimurium from the background by magnetic separation. CUR-TCO-Tz-pAb conjugates were reacted with the magnetic bacteria to form CUR-Tz-TCO bacteria. Finally, CUR was released quickly from the CUR-Tz-TCO bacteria in the presence of NaOH, and the color change was measured at the characteristic wavelength of 468 nm for bacteria quantification.Results: A linear relationship between absorbance at 468 nm and concentration of S. typhimurium from 102 to 106 CFU/mL was found. The lower detection limit was calculated to be as low as 50 CFU/mL and the mean recovery was 107.47% for S. typhimurium in spiked chicken samples.Conclusion: This biosensor has the potential for practical applications in the detection of foodborne pathogens.

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“…The potential of the SPR for Salmonella (live, dead, protein or DNA) detection, including S. Typhi and S. Typhimurium, has been widely studied [ 126 , 145 , 146 ], but there are no studies on SPR to detect the DNA of S. Paratyphi using specific DNA probes. Figure 4 illustrates a proposed SPR-based genoassay to detect S. Paratyphi, which can be explored in future studies.…”

Section: Discussionmentioning

confidence: 99%

Laboratory Diagnosis of Paratyphoid Fever: Opportunity of Surface Plasmon Resonance

Alhaj-Qasem¹,

Al-Hatamleh

Irekeola

et al. 2020

Diagnostics

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Paratyphoid fever is caused by the bacterium Salmonella enterica serovar Paratyphi (A, B and C), and contributes significantly to global disease burden. One of the major challenges in the diagnosis of paratyphoid fever is the lack of a proper gold standard. Given the absence of a licensed vaccine against S. Paratyphi, this diagnostic gap leads to inappropriate antibiotics use, thus, enhancing antimicrobial resistance. In addition, the symptoms of paratyphoid overlap with other infections, including the closely related typhoid fever. Since the development and utilization of a standard, sensitive, and accurate diagnostic method is essential in controlling any disease, this review discusses a new promising approach to aid the diagnosis of paratyphoid fever. This advocated approach is based on the use of surface plasmon resonance (SPR) biosensor and DNA probes to detect specific nucleic acid sequences of S. Paratyphi. We believe that this SPR-based genoassay can be a potent alternative to the current conventional diagnostic methods, and could become a rapid diagnostic tool for paratyphoid fever.

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A Portable Surface Plasmon Resonance Biosensor for Rapid Detection of Salmonella typhimurium

Cited by 20 publications

References 16 publications

On-Site Detection of Aflatoxin B1 in Grains by a Palm-Sized Surface Plasmon Resonance Sensor

On-Site Detection of Aflatoxin B1 in Grains by a Palm-Sized Surface Plasmon Resonance Sensor

An enzyme-free biosensor for sensitive detection of Salmonella using curcumin as signal reporter and click chemistry for signal amplification

Laboratory Diagnosis of Paratyphoid Fever: Opportunity of Surface Plasmon Resonance

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