A Potential Strategy for Simultaneous Determination of Deferoxamine and Vitamin C Using MCR-ALS with Nanostructured Electrochemical Sensor in Serum and Urine of Thalassemia and Diabetic Patients

Abstract

Simultaneous determination of deferoxamine and vitamin C at the surface of a nanostructured -modified sensor is reported at this study. The electrode is designed based on a carbon paste electrode (CPE) modified with 1-ethyl-3-methylimidazolium chloride as an ionic liquid (IL) and CdO-nanoparticle/rGO (IL/CdO/rGO/CPE). The nanostructures are studied by various techniques containing X-ray diffraction (XRD), energy dispersive X-ray (EDX), field emission scanning electron microscopes (FESEM), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). The high degree of overlapping voltammograms is studied by multivariate curve resolution alternating least (MCR-ALS) algorithm. At first, a multivariate optimization is studied using response surface methodology (RSM) and central composite rotatable design (CCRD) model based on a five-level, three factor to optimize various parameters on DPV signal of the drugs. Then, second order data are built by changing in pulse heights of DPV. Results of MCR-ALS are used to data analysis that is obtained by electro-oxidation of deferoxamine and vitamin C. The strategy shows the current method is a potential way for analysis of the two important drugs in the presence of each other with high voltammetric overlapping in human serum and urine samples of patients with thalassemia major and minor. The voltammetric analyses of deferoxamine and vitamin C are performed in the concentration range of 5.0 × 10−8−900.0 × 10−2 and 6.0 × 10−8−900.0 × 10−2 M, and the detection limits are calculated 0.030 × 10−6 and 0.025 × 10−6 M, respectively.

Bimetallic Biogenic Pt-Ag Nanoparticle and Their Application for Electrochemical Dopamine Sensor

“…Electrochemical methods are very promising in studies with sensors [34][35][36]. Electrical changes occur due to the reduction/oxidation reactions of the analyte, and different methods are used to analyze these electrical changes [37].…”

Thalassemia and Nanotheragnostics: Advanced Approaches for Diagnosis and Treatment

“…Another unique approach was adopted by Darabi et al to determine the vitamin C level and deferoxamine in thalassemic patients using a carbon paste-based electrode that was coupled to 1-ethyl-3-methylimidazolium chloride, as an ionic liquid (IL), and CdO-nanoparticle/rGO (IL/CdO/rGO/CPE) and was studied using a multivariate curve resolution alternating least squares (MCR-ALS) algorithm. This efficient analysis of the samples of both thalassemia major and minor patients with high accuracy demonstrated the efficacious use of carbon-based biosensors for further clinical investigations [ 76 ].…”

Electrochemical Behavior of Janus Kinase Inhibitor Ruxolitinib at a Taurine-Electropolymerized Carbon Paste Electrode: Insights into Sensing Mechanisms

“…In contrast, electrochemical methods offer a more environmentally friendly approach and are preferred for analyzing electroactive compounds in various fields, such as agriculture, food, pharmaceuticals, and healthcare. − Among electroanalytical techniques, voltammetry stands out as one of the most commonly used methods, particularly in drug analysis. These electrochemical methods offer several advantages, including shorter analysis times, lower costs, reduced use of organic solvents, high sensitivity, precise analytical capabilities, and the ability to analyze small sample volumes. − …”

“…These electrochemical methods offer several advantages, including shorter analysis times, lower costs, reduced use of organic solvents, high sensitivity, precise analytical capabilities, and the ability to analyze small sample volumes. 33 − 36 …”