A potential tool for diagnosis of male infertility: Plasma metabolomics based on GC–MS

Zhou, Xinyi; Wang, Yan; Yun, Yong‐Huan; Xia, Zian; Lü, Hongmei; Luo, Jie-Kun; Liang, Yi‐Zeng

doi:10.1016/j.talanta.2015.09.040

Cited by 40 publications

(27 citation statements)

References 44 publications

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“…The process of plasma sample preparation was performed as previously described [ 47 ]. Briefly, each 100 μl aliquot of the plasma samples from all rats were protein precipitated with 300 μl ice-cold methanol, followed by the addition of 30 μl C19:1/methanol (1 mg/ml) as the internal standard.…”

Section: Methodsmentioning

confidence: 99%

Metabolomics reveals the effect of Xuefu Zhuyu Decoction on plasma metabolism in rats with acute traumatic brain injury

et al. 2017

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Xuefu Zhuyu Decoction (XFZY), an important traditional Chinese herbal formula, has been reported effective on traumatic brain injury (TBI) in rats. However, its cerebral protection mechanism has not been clarified at the metabolic level. This work aims to explore the global metabolic characteristics of XFZY in rats during the acute phase of TBI on days 1 and 3. A plasma metabolomics method based on gas chromatography-mass spectrometry coupled with univariate analysis and multivariate statistical analysis was performed in three groups (Sham, Vehicle, XFZY). Then, a pathway analysis using MetaboAnalyst 3.0 was performed to illustrate the pathways of therapeutic action of XFZY in TBI. XFZY treatment attenuates neurological dysfunction and cortical lesion volume post-injury on day 3, and reverses the plasma metabolite abnormalities (glutamic acid, lactic acid, 3-hydroxybutyric acid, and ribitol, etc.). These differential metabolites are mainly involved in D-glutamine and D-glutamate metabolism, alanine, aspartate and glutamate metabolism, and inositol phosphate metabolism. Our study reveals potential biomarkers and metabolic networks of acute TBI and neuroprotection effects of XFZY, and shows this metabolomics approach with MetaboAnalyst would be a feasible way to systematically study therapeutic effects of XFZY on TBI.

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Section: Methodsmentioning

confidence: 99%

Metabolomics reveals the effect of Xuefu Zhuyu Decoction on plasma metabolism in rats with acute traumatic brain injury

et al. 2017

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“…To ensure data quality for metabolic profiling, quality control samples were prepared by pooling and mixing 35 μ L aliquots from each plasma sample. Quality control samples were used to assess and ensure that the analysis processes being performed are proper and the data acquired meet predefined acceptance criteria [ 17 ]. We tested the stability and repeatability of metabolomic method by the relative standard deviations (RSDs).…”

Section: Methodsmentioning

confidence: 99%

Plasma Metabolomics Analysis Based on GC‐MS in Infertile Males with Kidney‐Yang Deficiency Syndrome

Zheng

Wang

Lü

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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Introduction Chinese medicine syndrome diagnosis is the key requisite in the treatment of male infertility with traditional Chinese medicine (TCM). Kidney-Yang deficiency syndrome (KYDS) is the critical Chinese medicine syndrome of male infertility. To explore the modernized mechanisms of KYDS in male infertility, this study aims to investigate the metabolomics of males with KYDS. Methods The gas chromatography-mass spectrometry method was applied to analyze the plasma samples of 67 infertile males with KYDS compared with 55 age-matched healthy controls. The chemometric methods including principal component and partial least squares-discriminate analyses were employed to identify the potential biochemical patterns. With the help of the variable importance for the projection and receiver operating characteristic curve analyses, the potential biomarkers were extracted to define the clinical utility. Simultaneously the high-quality KEGG metabolic pathways database was used to identify the related metabolic pathways. Results The metabolomics profiles of infertile males with KYDS including 10 potential biomarkers and six metabolic pathways were identified. They precisely distinguished infertile males with KYDS from healthy controls. Conclusions These potential biomarkers and pathways suggest the substantial basis of infertile males with KYDS. The metabolomics profiles highlight the modernized mechanisms of infertile males with KYDS.

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“…Metabolite levels can then be compared between different phenotypic states and potentially be used as health indicators. Mass spectrometry-based metabolite analysis has been applied in the development of many informative biomarkers to help identify hard to diagnose disorders 10–13 . Recent studies have utilized metabolomic analysis in assessing embryo and oocyte quality 14–16 .…”

Section: Introductionmentioning

confidence: 99%

Plasma metabolomic profiles differ at the time of artificial insemination based on pregnancy outcome, in Bos taurus beef heifers

Phillips

Read

Kriese-Anderson

et al. 2018

Sci Rep

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Infertility remains the most prevalent reason for cattle being removed from production environments. We utilized metabolomic profiling to identify metabolites in the blood plasma that may be useful in identifying infertile heifers at the time of artificial insemination (AI). Prior to AI, phenotypic parameters including body condition, weight, and reproductive organ measurements were collected. These were determined not effective at differentiating between fertile and infertile heifers. Analysis of the resulting metabolomic profiles revealed 15 metabolites at significantly different levels (T-test P ≤ 0.05), with seven metabolites having a greater than 2-fold difference (T-test P ≤ 0.05, fold change ≥2, ROC-AUC ≥ 0.80) between infertile and fertile heifers. We further characterized the utility of using the levels of these metabolites in the blood plasma to discriminate between fertile and infertile heifers. Finally, we investigated the potential role inflammation may play by comparing the expression of inflammatory cytokines in the white blood cells of infertile heifers to that of fertile heifers. We found significantly higher expression in infertile heifers of the proinflammatory markers tumor necrosis factor alpha (TNFα), interleukin 6 (IL6), and the C-X-C motif chemokine 5 (CXCL5). Our work offers potentially valuable information regarding the diagnosis of fertility problems in heifers undergoing AI.

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A potential tool for diagnosis of male infertility: Plasma metabolomics based on GC–MS

Cited by 40 publications

References 44 publications

Metabolomics reveals the effect of Xuefu Zhuyu Decoction on plasma metabolism in rats with acute traumatic brain injury

Metabolomics reveals the effect of Xuefu Zhuyu Decoction on plasma metabolism in rats with acute traumatic brain injury

Plasma Metabolomics Analysis Based on GC‐MS in Infertile Males with Kidney‐Yang Deficiency Syndrome

Plasma metabolomic profiles differ at the time of artificial insemination based on pregnancy outcome, in Bos taurus beef heifers

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