A powerful Kjeldahl nitrogen method using peroxymonosulfuric acid

Hach, Clifford C.; Brayton, Scott V.; Kopelove, Alan B.

doi:10.1021/jf00066a025

Cited by 152 publications

(71 citation statements)

References 8 publications

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“…Urine (1 mL) was diluted 1:100 and analyzed by using an assay based on the Kjeldahl method (38). The CV for 8 blinded duplicate pairs was 2.2% for urinary nitrogen.…”

Section: Recovery Biomarkers: Dlw and Urinary Nitrogenmentioning

confidence: 99%

Dietary biomarker evaluation in a controlled feeding study in women from the Women’s Health Initiative cohort ,

Lampe

Huang

Neuhouser

et al. 2017

The American Journal of Clinical Nutrition

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Background: Controlled human feeding studies are necessary for robust nutritional biomarker development and validation. Previous feeding studies have typically evaluated single nutrients and tested relatively few diets. Objectives: The objectives were 1) to simultaneously associate dietary intake with a range of potential nutritional biomarkers in postmenopausal women by using a controlled feeding study whereby each participant was provided a diet similar to her usual diet and 2) to evaluate serum concentrations of select nutrients as potential biomarkers with the use of established urinary recovery biomarkers of energy and protein as benchmarks for evaluation. Design: Postmenopausal women from the Women's Health Initiative (n = 153) were provided with a 2-wk controlled diet in which each individual's menu approximated her habitual food intake as estimated from her 4-d food record and adjusted for estimated energy requirements. Serum biomarkers, including carotenoids, tocopherols, folate, vitamin B-12, and phospholipid fatty acids, were collected at the beginning and end of the feeding period. Doubly labeled water and urinary nitrogen biomarkers were used to derive estimates of energy and protein consumption, respectively. Results: Linear regression of (ln-transformed) consumed nutrients on (ln-transformed) potential biomarkers and participant characteristics led to the following regression (R 2 ) values for serum concentration biomarkers: folate, 0.49; vitamin B-12, 0.51; a-carotene, 0.53; b-carotene, 0.39; lutein + zeaxanthin, 0.46; lycopene, 0.32; and a-tocopherol, 0.47. R 2 values for percentage of energy from polyunsaturated fatty acids and urinary recovery biomarkers of energy and protein intakes were 0.27, 0.53, and 0.43, respectively. Phospholipid saturated fatty acids and monounsaturated fatty acids and serum g-tocopherol were weakly associated with intake (R 2 , 0.25). Conclusions: Serum concentration biomarkers of several vitamins and carotenoids performed similarly to established energy and protein urinary recovery biomarkers in representing nutrient intake variation in a feeding study, and thus are likely suitable for application in this population of postmenopausal women. Further work is needed to identify objective measures of categories of fatty acid intake. This trial was registered at clinicaltrials.gov as NCT00000611.Am J Clin Nutr 2017;105:466-75.

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“…Urine (1 mL) was diluted 1:100 and analyzed by using an assay based on the Kjeldahl method (38). The CV for 8 blinded duplicate pairs was 2.2% for urinary nitrogen.…”

Section: Recovery Biomarkers: Dlw and Urinary Nitrogenmentioning

confidence: 99%

Dietary biomarker evaluation in a controlled feeding study in women from the Women’s Health Initiative cohort ,

Lampe

Huang

Neuhouser

et al. 2017

The American Journal of Clinical Nutrition

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“…• Método rápido de determinación de proteínas de Hach [6]: consiste en una digestión de la muestra con ácido sulfúrico y peróxido de hidrógeno a 434°C. Luego, se realiza una determinación colorimétrica con el reactivo de Nessler y se lee a 460nm.…”

Section: -Determinación De Constituyentes Básicosunclassified

Caracterización de acelga fresca de Santiago del Estero (Argentina). Comparación del contenido de nutrientes en hoja y tallo. Evaluación de los carotenoides presentes

Costa¹,

Montenegro²,

Arregui³

et al. 2003

Ciênc. Tecnol. Aliment.

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“…All feed, ort, and fecal composite samples were analyzed for laboratory dry matter (DM) (105 °C for 8 h), OM [1], CP [5], ADF, NDF, and ADL [14].…”

Section: Resultsmentioning

confidence: 99%

“…pH was taken immediately. Ammonia nitrogen was determined spectrophotometrically using Nessler's reagent [5]. Volatile fatty acids were determined by gas chromatography.…”

Section: Resultsmentioning

confidence: 99%

Nutritional metabolism of hydrogen peroxide/anhydrous ammonia-treated barley straw in ewe lambs

Diouri

Wiedmeier

2000

Ann. Zootech.

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-A control and three treatments of barley straw were compared: NH 3 (3% of dry matter (DM)), 3% NH 3 after rehydration of the straw to 15% moisture with water, and 3% NH 3 after rehydration to 15% moisture with a H 2 O 2 solution (0.32% of DM). Forages were fed to fistulated ewe lambs, with a supplement, at two levels of intake (ad libitum and 75% ad libitum). At the ad libitum intake, the treatments mainly improved the intake; and digestible DM intake was 336, 455, 501, and 552 grams per head per day for untreated, NH 3 , NH 3 + H 2 O, and NH 3 + H 2 O 2 -treated straws, respectively. The first, second, and fourth values were different from each other (P < 0.01), but the third one was not different from the fourth (P = 0.09). A similar trend was observed in most other nutrient utilization parameters and fermentation characteristics. Intake restriction raised DM digestibility from 56.9% to 58.2% (P = 0.04). Anhydrous ammonia cannot provide alkalinity for the optimal action of hydrogen peroxide. straw / treatment / ammonia / hydrogen peroxide Résumé -Métabolisme alimentaire de la paille d'orge traitée à l'ammoniac anhydre et/ou à l'eau oxygénée chez les agnelles. Un témoin et trois traitements de paille d'orge : NH 3 (3 % MS), 3 % NH 3 après réhydratation de la paille à 15 % d'humidité avec de l'eau, et 3 % NH 3 après réhy-dratation à 15 % d'humidité avec une solution de H 2 O 2 (0,32 % MS) ont été comparés. Ces pailles ont été distribuées, à 8 agnelles fistulées, à deux niveaux d'ingestion (ad libitum et 75 % ad libitum), selon un dispositif en carré latin. Lorsque l'ingestion était à volonté, les traitements ont agi principalement sur l'ingestibilité, et la MS digestible a été de 336, 455, 501, et 552 grammes par tête par jour pour le témoin, les pailles traitées à NH 3 , NH 3 + H 2 O, et NH 3 + H 2 O 2 respectivement. Les première, deuxième, et quatrième valeurs ont été significativement différentes l'une de l'autre (P < 0,01) ; alors que la troisième ne l'a pas été de la quatrième (P = 0,09). Une tendance semblable a été observée pour la plupart des autres paramètres de digestion. La digestibilité de la MS est passée, en moyenne, de 56,9 % à 58,2 % avec la restriction de l'ingestion (P = 0,04). L'ammoniac ne peut pas fournir une alcalinité suffisante pour une action optimale de l'eau oxygénée.paille / traitement / ammoniac / eau oxygénée Ann. Zootech. 49 (2000) 329-333 329 © INRA, EDP Sciences

show abstract

A powerful Kjeldahl nitrogen method using peroxymonosulfuric acid

Cited by 152 publications

References 8 publications

Dietary biomarker evaluation in a controlled feeding study in women from the Women’s Health Initiative cohort ,

Dietary biomarker evaluation in a controlled feeding study in women from the Women’s Health Initiative cohort ,

Caracterización de acelga fresca de Santiago del Estero (Argentina). Comparación del contenido de nutrientes en hoja y tallo. Evaluación de los carotenoides presentes

Nutritional metabolism of hydrogen peroxide/anhydrous ammonia-treated barley straw in ewe lambs

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